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HSPA8 elisa kit :: Rat Heat Shock 70kDa Protein 8 (HSPA8) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2024327 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $445 +1 FREE 8GB USB
96-Strip-Wells  /  $595 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,390 +2 FREE 8GB USB
10x96-Strip-Wells  /  $4,305 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Heat Shock 70kDa Protein 8 (HSPA8), ELISA Kit

 Also Known As   

Heat Shock 70kDa Protein 8 (HSPA8) ELISA Kit

 Product Synonym Names    LAP1; HSC54; HSC70; HSC71; HSP71; HSPA10; NIP71; HSP73; Heat shock cognate 71 kDa protein; Lipopolysaccharide-associated protein 1; LPS-associated protein 1
 Product Gene Name   

HSPA8 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    600816
 3D Structure    ModBase 3D Structure for P11142
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Heat Shock 70kDa Protein 8 (HSPA8).
No significant cross-reactivity or interference between Heat Shock 70kDa Protein 8 (HSPA8) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma and Other Biological Fluids
 Detection Range    0.312-20ng/mL
 Sensitivity    < 0.103ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Heat Shock 70kDa Protein 8 (HSPA8) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Heat Shock 70kDa Protein 8 (HSPA8) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HSPA8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HSPA8 purchase    MBS2024327 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Heat Shock 70kDa Protein 8 (HSPA8) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20ng/mL in biological research samples containing HSPA8, with an estimated sensitivity of < 0.103ng/mL. The ELISA analytical biochemical technique of the MBS2024327 kit is based on HSPA8 antibody-HSPA8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HSPA8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HSPA8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for HSPA8 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 8 (HSPA8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 70kDa Protein 8 (HSPA8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 70kDa Protein 8 (HSPA8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock 70kDa Protein 8 (HSPA8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for HSPA8 elisa kit    Signal transduction
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 Typical Testing Data/Standard Curve (for reference only) of HSPA8 elisa kit    HSPA8 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2024327. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HSPA8. It may not necessarily be applicable to this product.
 NCBI GI #    18043726
 NCBI GeneID    3312
 NCBI Accession #    AAH19816.1 [Other Products]
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 UniProt Primary Accession #    P11142 [Other Products]
 UniProt Secondary Accession #    Q9H3R6 [Other Products]
 UniProt Related Accession #    P11142 [Other Products]
 Molecular Weight    53,518 Da
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 NCBI Official Full Name    Heat shock 70kDa protein 8
 NCBI Official Synonym Full Names    heat shock 70kDa protein 8
 NCBI Official Symbol    HSPA8 [Similar Products]
 NCBI Official Synonym Symbols   
LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; LAP-1; NIP71; HEL-33; HSPA10; HEL-S-72p
[Similar Products]
 NCBI Protein Information    heat shock cognate 71 kDa protein
 UniProt Protein Name    Heat shock cognate 71 kDa protein
 UniProt Synonym Protein Names   
Heat shock 70 kDa protein 8; Lipopolysaccharide-associated protein 1; LAP-1; LPS-associated protein 1
 UniProt Gene Name    HSPA8 [Similar Products]
 UniProt Synonym Gene Names    HSC70; HSP73; HSPA10; LAP-1; LPS-associated protein 1 [Similar Products]
 UniProt Entry Name    HSP7C_HUMAN
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 NCBI Summary for HSPA8    This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
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 UniProt Comments for HSPA8    HSC70: Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co- chaperones. Interacts with HSPH1/HSP105. Interacts with IRAK1BP1. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with PACRG and TSC2. Interacts with BAG1. Interacts with SV40 VP1. Interacts with DNAJC7. Interacts with HERC5. Interacts with CITED1 (via N-terminus); the interaction suppresses the association of CITED1 to p300/CBP and Smad-mediated transcription transactivation. Constitutively synthesized. Ubiquitous. Belongs to the heat shock protein 70 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; Chaperone; Heat shock protein; RNA-binding

Chromosomal Location of Human Ortholog: 11q24.1

Cellular Component: nucleoplasm; spliceosome; extracellular space; focal adhesion; membrane; plasma membrane; nucleolus; melanosome; intracellular; cytosol; ribonucleoprotein complex; nucleus; ubiquitin ligase complex

Molecular Function: protein binding; enzyme binding; G-protein-coupled receptor binding; heat shock protein binding; ubiquitin protein ligase binding; unfolded protein binding; ATPase activity; ATPase activity, coupled; ATP binding

Biological Process: axon guidance; viral reproduction; transcription, DNA-dependent; chaperone cofactor-dependent protein folding; protein folding; regulation of cell cycle; RNA splicing; neurotransmitter secretion; response to unfolded protein; positive regulation of nuclear mRNA splicing, via spliceosome; nuclear mRNA splicing, via spliceosome; synaptic transmission; gene expression; protein refolding; post-Golgi vesicle-mediated transport; negative regulation of transcription, DNA-dependent
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 Research Articles on HSPA8    1. Hsc70 interacted with Rab1A in a chaperone-dependent mannerand Hsc70 knockdown decreased the level of Rab1A and increased the level of its ubiquitination under stress conditions.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HSPA8 elisa kitPathways associated with HSPA8 elisa kit
 Reference Product  PubMed Publications
 DNAJB1 elisa kit  >22 publications with HSPA8 and DNAJB1
 STUB1 elisa kit  >19 publications with HSPA8 and STUB1
 CFTR elisa kit  >15 publications with HSPA8 and CFTR
 HSP90AA1 elisa kit  >6 publications with HSPA8 and HSP90AA1
 BAG1 elisa kit  >5 publications with HSPA8 and BAG1
 BAG2 elisa kit  >2 publications with HSPA8 and BAG2
 Products by Pathway  Pathway Diagram
 AUF1 (hnRNP D0) Destabilizes MRNA Pathway antibodies  AUF1 (hnRNP D0) Destabilizes MRNA Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Attenuation Phase Pathway antibodies  Attenuation Phase Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 CHL1 Interactions Pathway antibodies  CHL1 Interactions Pathway Diagram
 Cellular Response To Heat Stress Pathway antibodies  Cellular Response To Heat Stress Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Clathrin Derived Vesicle Budding Pathway antibodies  Clathrin Derived Vesicle Budding Pathway Diagram
Diseases associated with HSPA8 elisa kitOrgans/Tissues associated with HSPA8 elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >56 publications with HSPA8 and Nervous System Diseases
 Brain Diseases Antibodies  >42 publications with HSPA8 and Brain Diseases
 Cardiovascular Diseases Antibodies  >27 publications with HSPA8 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >24 publications with HSPA8 and Disease Models, Animal
 Fibrosis Antibodies  >17 publications with HSPA8 and Fibrosis
 Heart Diseases Antibodies  >17 publications with HSPA8 and Heart Diseases
 Necrosis Antibodies  >16 publications with HSPA8 and Necrosis
 Inflammation Antibodies  >14 publications with HSPA8 and Inflammation
 Liver Diseases Antibodies  >11 publications with HSPA8 and Liver Diseases
 Adenocarcinoma Antibodies  >9 publications with HSPA8 and Adenocarcinoma
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >97 publications with HSPA8 and Blood
 Brain Antibodies  >96 publications with HSPA8 and Brain
 Muscle Antibodies  >49 publications with HSPA8 and Muscle
 Liver Antibodies  >46 publications with HSPA8 and Liver
 Nerve Antibodies  >42 publications with HSPA8 and Nerve
 Heart Antibodies  >36 publications with HSPA8 and Heart
 Kidney Antibodies  >29 publications with HSPA8 and Kidney
 Vascular Antibodies  >18 publications with HSPA8 and Vascular
 Lung Antibodies  >14 publications with HSPA8 and Lung
 Bone Antibodies  >11 publications with HSPA8 and Bone
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