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HMGB-1 elisa kit :: Rat High mobility group protein B1 (HMGB-1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS265968
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $275 +1 FREE 8GB USB
96-Strip-Wells  /  $435 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

High mobility group protein B1 (HMGB-1), ELISA Kit

★Popular Item★
 Also Known As   

Rat High mobility group protein B1 (HMGB-1) ELISA Kit

 Product Gene Name   

HMGB-1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    163905
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 Species Reactivity    Rat
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 Specificity    No cross-reaction with other factors.
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 Samples    Rat serum, plasma or cell culture supernatant and organizations in the natural and recombinant HMGB-1 concentration
 Assay Type    Sandwich
 Detection Range    2000 pg/ml-31.2 pg/ml
 Sensitivity    12 pg/ml
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HMGB-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HMGB-1 purchase    MBS265968 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the High mobility group protein B1 (HMGB-1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 2000 pg/ml-31.2 pg/ml in biological research samples containing HMGB-1, with an estimated sensitivity of 12 pg/ml. The ELISA analytical biochemical technique of the MBS265968 kit is based on HMGB-1 antibody-HMGB-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HMGB-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HMGB-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Rat serum, plasma or cell culture supernatant and organizations in the natural and recombinant HMGB-1 concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for HMGB-1 elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat HMGB-1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of HMGB-1 elisa kit    HMGB-1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS265968. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HMGB-1. It may not necessarily be applicable to this product.
 NCBI GI #    4504425
 NCBI GeneID    3146
 NCBI Accession #    NP_002119.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002128.4 [Other Products]
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 UniProt Secondary Accession #    Q14321; Q5T7C3; Q6IBE1; A5D8W9 [Other Products]
 UniProt Related Accession #    P09429 [Other Products]
 Molecular Weight    24,894 Da
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 NCBI Official Full Name    high mobility group protein B1
 NCBI Official Synonym Full Names    high mobility group box 1
 NCBI Official Symbol    HMGB1 [Similar Products]
 NCBI Official Synonym Symbols   
HMG1; HMG3; SBP-1
[Similar Products]
 NCBI Protein Information    high mobility group protein B1; HMG-1; Amphoterin; high-mobility group box 1; high mobility group protein 1; Sulfoglucuronyl carbohydrate binding protein; high-mobility group (nonhistone chromosomal) protein 1
 UniProt Protein Name    High mobility group protein B1
 UniProt Synonym Protein Names   
High mobility group protein 1; HMG-1
 UniProt Gene Name    HMGB1 [Similar Products]
 UniProt Synonym Gene Names    HMG1; HMG-1 [Similar Products]
 UniProt Entry Name    HMGB1_HUMAN
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 UniProt Comments for HMGB-1    HMGB1: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Belongs to the HMGB family.

Protein type: DNA repair, damage; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: nucleoplasm; extracellular space; cell surface; transcriptional repressor complex; extracellular region; condensed chromosome; nucleus

Molecular Function: protein binding; RAGE receptor binding; double-stranded DNA binding; damaged DNA binding; cytokine activity; chromatin binding; transcription factor activity; transcription factor binding; DNA bending activity; single-stranded DNA binding; chemoattractant activity

Biological Process: negative regulation of transcriptional preinitiation complex assembly; DNA topological change; V(D)J recombination; positive regulation of apoptosis; apoptosis; positive regulation of caspase activity; base-excision repair, DNA ligation; negative regulation of transcription from RNA polymerase II promoter; DNA recombination; chromatin remodeling; regulation of transcription from RNA polymerase II promoter; myeloid dendritic cell activation; inflammatory response to antigenic stimulus; positive chemotaxis; innate immune response; DNA ligation during DNA repair; dendritic cell chemotaxis; positive regulation of transcription from RNA polymerase II promoter; DNA fragmentation during apoptosis; neurite development; cell structure disassembly during apoptosis; positive regulation of DNA binding
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 Research Articles on HMGB-1    1. The ectopic expression of HMGB1 contributes to enhancing metastatic potential via disturbances with the FAK/PI3K/Akt signaling pathways.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HMGB-1 elisa kitPathways associated with HMGB-1 elisa kit
 Reference Product  PubMed Publications
 TNF elisa kit  >338 publications with HMGB-1 and TNF
 TLR4 elisa kit  >241 publications with HMGB-1 and TLR4
 TP53 elisa kit  >6 publications with HMGB-1 and TP53
 DIF elisa kit  >2 publications with HMGB-1 and DIF
 PLAT elisa kit  >1 publications with HMGB-1 and PLAT
 AGER elisa kit  >1 publications with HMGB-1 and AGER
 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of DNA Fragmentation Factor Pathway antibodies  Activation Of DNA Fragmentation Factor Pathway Diagram
 Advanced Glycosylation Endproduct Receptor Signaling Pathway antibodies  Advanced Glycosylation Endproduct Receptor Signaling Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Induced DNA Fragmentation Pathway antibodies  Apoptosis Induced DNA Fragmentation Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Cytosolic Sensors Of Pathogen-associated DNA Pathway antibodies  Cytosolic Sensors Of Pathogen-associated DNA Pathway Diagram
 DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway antibodies  DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway Diagram
Diseases associated with HMGB-1 elisa kitOrgans/Tissues associated with HMGB-1 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >986 publications with HMGB-1 and Inflammation
 Necrosis Antibodies  >573 publications with HMGB-1 and Necrosis
 Sepsis Antibodies  >405 publications with HMGB-1 and Sepsis
 Neoplasms Antibodies  >389 publications with HMGB-1 and Neoplasms
 Disease Models, Animal Antibodies  >280 publications with HMGB-1 and Disease Models, Animal
 Lung Diseases Antibodies  >163 publications with HMGB-1 and Lung Diseases
 Liver Diseases Antibodies  >119 publications with HMGB-1 and Liver Diseases
 Drug Toxicity Antibodies  >87 publications with HMGB-1 and Drug Toxicity
 Heart Diseases Antibodies  >83 publications with HMGB-1 and Heart Diseases
 Myocardial Ischemia Antibodies  >70 publications with HMGB-1 and Myocardial Ischemia
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >858 publications with HMGB-1 and Blood
 Liver Antibodies  >307 publications with HMGB-1 and Liver
 Lung Antibodies  >276 publications with HMGB-1 and Lung
 Brain Antibodies  >223 publications with HMGB-1 and Brain
 Vascular Antibodies  >218 publications with HMGB-1 and Vascular
 Muscle Antibodies  >129 publications with HMGB-1 and Muscle
 Heart Antibodies  >113 publications with HMGB-1 and Heart
 Kidney Antibodies  >108 publications with HMGB-1 and Kidney
 Bone Antibodies  >94 publications with HMGB-1 and Bone
 Nerve Antibodies  >92 publications with HMGB-1 and Nerve
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