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HIS elisa kit :: Rat Histamine (HIS) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS260988
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $275 +1 FREE 8GB USB
96-Strip-Wells  /  $435 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Histamine (HIS), ELISA Kit

★Popular Item★
 Also Known As   

Rat Histamine (HIS) ELISA Kit

 Product Gene Name   

HIS elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    142703
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 Species Reactivity    Rat
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 Specificity    No cross-reaction with other factors.
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 Samples    Rat serum, plasma or cell culture supernatant and organizations in the natural and recombinant HIS concentration.
 Assay Type    Sandwich
 Detection Range    50 ng/ml-0.78 ng/ml
 Sensitivity    The minimum detectable Rat HIS up to 0.08 ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HIS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HIS purchase    MBS260988 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Histamine (HIS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 50 ng/ml-0.78 ng/ml in biological research samples containing HIS, with an estimated sensitivity of The minimum detectable Rat HIS up to 0.08 ng/ml. The ELISA analytical biochemical technique of the MBS260988 kit is based on HIS antibody-HIS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HIS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HIS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Rat serum, plasma or cell culture supernatant and organizations in the natural and recombinant HIS concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for HIS elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat HIS monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of HIS elisa kit    HIS elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS260988. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HIS. It may not necessarily be applicable to this product.
 NCBI GI #    197085610
 NCBI GeneID    3274
 NCBI Accession #    NP_001124527.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001131055.1 [Other Products]
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 UniProt Secondary Accession #    Q14464; Q7Z5R9; B5BUP7 [Other Products]
 UniProt Related Accession #    P25021 [Other Products]
 Molecular Weight    44,541 Da
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 NCBI Official Full Name    histamine H2 receptor isoform 1
 NCBI Official Synonym Full Names    histamine receptor H2
 NCBI Official Symbol    HRH2 [Similar Products]
 NCBI Official Synonym Symbols   
H2R
[Similar Products]
 NCBI Protein Information    histamine H2 receptor; HH2R; gastric receptor 1; gastric receptor I
 UniProt Protein Name    Histamine H2 receptor
 UniProt Synonym Protein Names   
Gastric receptor I
 UniProt Gene Name    HRH2 [Similar Products]
 UniProt Synonym Gene Names    H2R; HH2R [Similar Products]
 UniProt Entry Name    HRH2_HUMAN
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 NCBI Summary for HIS    Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H2 belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and stimulates gastric acid secretion. It also regulates gastrointestinal motility and intestinal secretion and is thought to be involved in regulating cell growth and differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
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 UniProt Comments for HIS    H2R: The H2 subclass of histamine receptors mediates gastric acid secretion. Also appears to regulate gastrointestinal motility and intestinal secretion. Possible role in regulating cell growth and differentiation. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and, through a separate G protein-dependent mechanism, the phosphoinositide/protein kinase (PKC) signaling pathway. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; GPCR, family 1

Chromosomal Location of Human Ortholog: 5q35.2

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: histamine receptor activity

Biological Process: gland development; G-protein signaling, coupled to cyclic nucleotide second messenger; gastrin-induced gastric acid secretion; histamine-induced gastric acid secretion; gut development; positive regulation of vasoconstriction; visual learning; regulation of synaptic plasticity; immune response; memory
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 Research Articles on HIS    1. But carriers of one or three copies of HRH1 (5% of individuals), HRH2 (1.1%) and HRH4 genes (0.9%) were also identified.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HIS elisa kitPathways associated with HIS elisa kit
 Reference Product  PubMed Publications
 JUN elisa kit  >4 publications with HIS and JUN
 HDC elisa kit  >2 publications with HIS and HDC
 ATP4A elisa kit  >2 publications with HIS and ATP4A
 Products by Pathway  Pathway Diagram
 Amine Ligand-binding Receptors Pathway antibodies  Amine Ligand-binding Receptors Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 Gastric Acid Secretion Pathway antibodies  Gastric Acid Secretion Pathway Diagram
 Gastric Acid Secretion Pathway antibodies  Gastric Acid Secretion Pathway Diagram
Diseases associated with HIS elisa kitOrgans/Tissues associated with HIS elisa kit
 Disease Name  Pubmed Publications
 Drug Hypersensitivity Antibodies  >3 publications with HIS and Drug Hypersensitivity
 Inflammation Antibodies  >2 publications with HIS and Inflammation
 Pain Antibodies  >2 publications with HIS and Pain
 Seizures Antibodies  >2 publications with HIS and Seizures
 Neoplasms, Experimental Antibodies  >2 publications with HIS and Neoplasms, Experimental
 Ulcer Antibodies  >1 publications with HIS and Ulcer
 Peptic Ulcer Antibodies  >1 publications with HIS and Peptic Ulcer
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3 publications with HIS and Brain
 Skin Antibodies  >2 publications with HIS and Skin
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