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Lipe elisa kit :: Rat Hormone-sensitive lipase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS762158 (SPECIAL PRICING: 1 unit is in stock at 40% OFF web price.
Please contact customer service for more information)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $415 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Hormone-sensitive lipase (Lipe), ELISA Kit

★Popular Item★
 Also Known As   

Rat Hormone-sensitive lipase ELISA Kit

 Product Synonym Names    Lipe/HSL/LHS/LIPE/hormone-sensitive lipase/hormone-sensitive lipase testicular isoform/HSL/HSLEC; hormone-sensitive/LIPE
 Product Gene Name   

Lipe elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS762158 COA    COA PDF
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Lipe . No significant cross-reactivity or interference between Lipe and analogues was observed.
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 Samples    Cell
 Assay Type    Sandwich
 Detection Range    31.2-2000pg/ml
 Sensitivity    <2pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipe were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipe were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Lipe elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Lipe purchase    MBS762158 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hormone-sensitive lipase (Lipe) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.2-2000pg/ml in biological research samples containing Lipe, with an estimated sensitivity of <2pg/ml. The ELISA analytical biochemical technique of the MBS762158 kit is based on Lipe antibody-Lipe antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Lipe antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Lipe. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Lipe elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Lipe antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Lipe antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Lipe amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Lipe can be calculated.
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 Typical Testing Data/Standard Curve (for reference only) of Lipe elisa kit    Lipe elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS762158. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Lipe. It may not necessarily be applicable to this product.
 NCBI GI #    87239972
 NCBI GeneID    16890
 NCBI Accession #    NP_001034596.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001039507.2 [Other Products]
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 UniProt Secondary Accession #    P97866; Q3TE34; Q6GU16; Q8CDI9 [Other Products]
 UniProt Related Accession #    P54310 [Other Products]
 Molecular Weight    88,027 Da
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 NCBI Official Full Name    hormone-sensitive lipase isoform 2
 NCBI Official Synonym Full Names    lipase, hormone sensitive
 NCBI Official Symbol    Lipe [Similar Products]
 NCBI Official Synonym Symbols   
HSL; 4933403G17Rik
[Similar Products]
 NCBI Protein Information    hormone-sensitive lipase
 UniProt Protein Name    Hormone-sensitive lipase
 Protein Family    Hormone-sensitive lipase
 UniProt Gene Name    Lipe [Similar Products]
 UniProt Synonym Gene Names    HSL [Similar Products]
 UniProt Entry Name    LIPS_MOUSE
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 UniProt Comments for Lipe    HSL: hormone sensitive lipase is a lipolytic enzyme of the 'GDXG' family. Plays a rate limiting step in triglyceride lipolysis. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. Rapidly activated by cAMP-dependent phosphorylation under the influence of catecholamines. Dephosphorylation and inactivation are controlled by insulin.

Protein type: Lipase; EC

Cellular Component: caveola; cytoplasm; cytosol; extracellular space; lipid particle; membrane; mitochondrion; nucleus; plasma membrane

Molecular Function: acylglycerol lipase activity; hormone-sensitive lipase activity; hydrolase activity; hydrolase activity, acting on ester bonds; lipase activity; protein binding; protein kinase binding; rRNA primary transcript binding; triacylglycerol lipase activity

Biological Process: cholesterol metabolic process; diacylglycerol catabolic process; lipid catabolic process; lipid metabolic process; long-chain fatty acid catabolic process; metabolic process; steroid metabolic process; termination of RNA polymerase I transcription; transcription initiation from RNA polymerase I promoter; triacylglycerol catabolic process
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 Research Articles on Lipe    1. Data suggest that cardiotrophin-1 up-regulates lipolysis in white adipocytes via 1) induction of perilipin, 2) activation of HSL (via phosphorylation by PKA), and 3) inactivation of adipose triglyceride lipase (via up-regulation of inhibitor G0S2).
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Lipe elisa kitDiseases associated with Lipe elisa kit
 Reference Product  PubMed Publications
 Fabp4 elisa kit  >5 publications with Lipe and Fabp4
 Plin1 elisa kit  >4 publications with Lipe and Plin1
 Mgll elisa kit  >3 publications with Lipe and Mgll
 Prkaa1 elisa kit  >1 publications with Lipe and Prkaa1
 Prkaa2 elisa kit  >1 publications with Lipe and Prkaa2
 Prkaca elisa kit  >1 publications with Lipe and Prkaca
 Disease Name  Pubmed Publications
 Obesity Antibodies  >23 publications with Lipe and Obesity
 Cognition Disorders Antibodies  >19 publications with Lipe and Cognition Disorders
 Insulin Resistance Antibodies  >16 publications with Lipe and Insulin Resistance
 Cardiovascular Diseases Antibodies  >12 publications with Lipe and Cardiovascular Diseases
 Fatty Liver Antibodies  >9 publications with Lipe and Fatty Liver
 Atherosclerosis Antibodies  >7 publications with Lipe and Atherosclerosis
 Diabetes Mellitus Antibodies  >6 publications with Lipe and Diabetes Mellitus
 Weight Gain Antibodies  >5 publications with Lipe and Weight Gain
 Infertility, Male Antibodies  >5 publications with Lipe and Infertility, Male
 Hypertension Antibodies  >5 publications with Lipe and Hypertension
Organs/Tissues associated with Lipe elisa kit
 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >5 publications with Lipe and Testis
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