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HIF1a elisa kit :: Rat Hypoxia Inducible Factor 1 Alpha (HIF1a) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2702491 (SPECIAL PROMOTIONAL PRICING for a limited time)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $220 +1 FREE 8GB USB
48-Strip-Wells  /  $350 +1 FREE 8GB USB
96-Strip-Wells  /  $470 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,960 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,625 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Hypoxia Inducible Factor 1 Alpha (HIF1a), ELISA Kit

★Popular Item★
 Also Known As   

Rat Hypoxia Inducible Factor 1 Alpha (HIF1a) ELISA Kit

 Product Synonym Names    HIF1-A; MOP1; PASD8; Basic Helix-Loop-Helix Transcription Factor; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78
 Product Gene Name   

HIF1a elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    U22431 mRNA
 3D Structure    ModBase 3D Structure for Q16665
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Hypoxia Inducible Factor 1 Alpha (HIF1a).
No significant cross-reactivity or interference between Hypoxia Inducible Factor 1 Alpha (HIF1a) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
 Detection Range    0.156-10ng/mL
 Sensitivity    < 0.056ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Hypoxia Inducible Factor 1 Alpha (HIF1a) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Hypoxia Inducible Factor 1 Alpha (HIF1a) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HIF1a elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HIF1a purchase    MBS2702491 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hypoxia Inducible Factor 1 Alpha (HIF1a) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing HIF1a, with an estimated sensitivity of < 0.056ng/mL. The ELISA analytical biochemical technique of the MBS2702491 kit is based on HIF1a antibody-HIF1a antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HIF1a antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HIF1a. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for HIF1a elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hypoxia Inducible Factor 1 Alpha (HIF1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hypoxia Inducible Factor 1 Alpha (HIF1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hypoxia Inducible Factor 1 Alpha (HIF1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hypoxia Inducible Factor 1 Alpha (HIF1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of HIF1a elisa kit    HIF1a elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2702491. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HIF1a. It may not necessarily be applicable to this product.
 NCBI GI #    4504385
 NCBI GeneID    3091
 NCBI Accession #    NP_001521.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001530.3 [Other Products]
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 UniProt Primary Accession #    Q16665 [Other Products]
 UniProt Secondary Accession #    Q53XP6; Q96PT9; Q9UPB1; C0LZJ3 [Other Products]
 UniProt Related Accession #    Q16665 [Other Products]
 Molecular Weight    95,634 Da
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 NCBI Official Full Name    hypoxia-inducible factor 1-alpha isoform 1
 NCBI Official Synonym Full Names    hypoxia inducible factor 1 alpha subunit
 NCBI Official Symbol    HIF1A [Similar Products]
 NCBI Official Synonym Symbols   
HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
[Similar Products]
 NCBI Protein Information    hypoxia-inducible factor 1-alpha
 UniProt Protein Name    Hypoxia-inducible factor 1-alpha
 UniProt Synonym Protein Names   
ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1
 Protein Family    Hypoxia-inducible factor
 UniProt Gene Name    HIF1A [Similar Products]
 UniProt Synonym Gene Names    bHLHe78 [Similar Products]
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 NCBI Summary for HIF1a    This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011]
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 UniProt Comments for HIF1a    Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.
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 Research Articles on HIF1a    1. Data show that NF-kappaB/p65 is required for the binding of zinc finger protein 91 (ZFP91) to the HIF-1alpha promoter and for the transcriptional activation of HIF-1alpha gene mediated by ZFP91.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HIF1a elisa kitPathways associated with HIF1a elisa kit
 Reference Product  PubMed Publications
 VHL elisa kit  >469 publications with HIF1a and VHL
 ARNT elisa kit  >265 publications with HIF1a and ARNT
 EGLN1 elisa kit  >116 publications with HIF1a and EGLN1
 EGLN3 elisa kit  >60 publications with HIF1a and EGLN3
 HIF1AN elisa kit  >55 publications with HIF1a and HIF1AN
 TP53 elisa kit  >29 publications with HIF1a and TP53
 EP300 elisa kit  >21 publications with HIF1a and EP300
 CREBBP elisa kit  >13 publications with HIF1a and CREBBP
 UBC elisa kit  >4 publications with HIF1a and UBC
 HSP90AA1 elisa kit  >3 publications with HIF1a and HSP90AA1
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Angiogenesis Pathway antibodies  Angiogenesis Pathway Diagram
 Cellular Response To Hypoxia Pathway antibodies  Cellular Response To Hypoxia Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Circadian Clock Pathway antibodies  Circadian Clock Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 FBXW7 Mutants And NOTCH1 In Cancer Pathway antibodies  FBXW7 Mutants And NOTCH1 In Cancer Pathway Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
Diseases associated with HIF1a elisa kitOrgans/Tissues associated with HIF1a elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >2859 publications with HIF1a and Neoplasms
 Carcinoma Antibodies  >1194 publications with HIF1a and Carcinoma
 Nervous System Diseases Antibodies  >574 publications with HIF1a and Nervous System Diseases
 Inflammation Antibodies  >430 publications with HIF1a and Inflammation
 Brain Diseases Antibodies  >420 publications with HIF1a and Brain Diseases
 Breast Neoplasms Antibodies  >369 publications with HIF1a and Breast Neoplasms
 Kidney Diseases Antibodies  >368 publications with HIF1a and Kidney Diseases
 Lung Diseases Antibodies  >344 publications with HIF1a and Lung Diseases
 Necrosis Antibodies  >321 publications with HIF1a and Necrosis
 Liver Diseases Antibodies  >314 publications with HIF1a and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >634 publications with HIF1a and Brain
 Muscle Antibodies  >625 publications with HIF1a and Muscle
 Kidney Antibodies  >594 publications with HIF1a and Kidney
 Lung Antibodies  >519 publications with HIF1a and Lung
 Liver Antibodies  >508 publications with HIF1a and Liver
 Bone Antibodies  >356 publications with HIF1a and Bone
 Prostate Antibodies  >249 publications with HIF1a and Prostate
 Embryonic Tissue Antibodies  >247 publications with HIF1a and Embryonic Tissue
 Bone Marrow Antibodies  >160 publications with HIF1a and Bone Marrow
 Lymph Node Antibodies  >148 publications with HIF1a and Lymph Node
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