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HIF2a elisa kit :: Rat Hypoxia Inducible Factor 2 Alpha (HIF2a) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2020870 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $285 +1 FREE 8GB USB
48-Strip-Wells  /  $530 +1 FREE 8GB USB
96-Strip-Wells  /  $715 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,910 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,280 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Hypoxia Inducible Factor 2 Alpha (HIF2a), ELISA Kit

★Popular Item★
 Also Known As   

Hypoxia Inducible Factor 2 Alpha (HIF2a) ELISA Kit

 Product Synonym Names    EPAS1; HLF; MOP2; PASD2; BHLHE73; Endothelial PAS Domain 1; HIF-1 Alpha-Like Factor; Basic-helix-loop-helix-PAS protein MOP2; Class E basic helix-loop-helix protein 73
 Product Gene Name   

HIF2a elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q9JHS1
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Hypoxia Inducible Factor 2 Alpha (HIF2a).
No significant cross-reactivity or interference between Hypoxia Inducible Factor 2 Alpha (HIF2a) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
 Detection Range    78-5000pg/mL
 Sensitivity    < 29pg/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Hypoxia Inducible Factor 2 Alpha (HIF2a) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Hypoxia Inducible Factor 2 Alpha (HIF2a) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HIF2a elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HIF2a purchase    MBS2020870 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hypoxia Inducible Factor 2 Alpha (HIF2a) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 78-5000pg/mL in biological research samples containing HIF2a, with an estimated sensitivity of < 29pg/mL. The ELISA analytical biochemical technique of the MBS2020870 kit is based on HIF2a antibody-HIF2a antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HIF2a antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HIF2a. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for HIF2a elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hypoxia Inducible Factor 2 Alpha (HIF2a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hypoxia Inducible Factor 2 Alpha (HIF2a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hypoxia Inducible Factor 2 Alpha (HIF2a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hypoxia Inducible Factor 2 Alpha (HIF2a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for HIF2a elisa kit    Cardiovascular biology
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 Typical Testing Data/Standard Curve (for reference only) of HIF2a elisa kit    HIF2a elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2020870. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HIF2a. It may not necessarily be applicable to this product.
 NCBI GI #    8953577
 NCBI GeneID    29452
 NCBI Accession #    CAB96612.1 [Other Products]
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 UniProt Primary Accession #    Q9JHS1 [Other Products]
 UniProt Related Accession #    Q9JHS1 [Other Products]
 Molecular Weight    96,718 Da
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 NCBI Official Full Name    hypoxia inducible factor 2 alpha
 NCBI Official Synonym Full Names    endothelial PAS domain protein 1
 NCBI Official Symbol    Epas1 [Similar Products]
 NCBI Official Synonym Symbols   
HLF; Hif2a
[Similar Products]
 NCBI Protein Information    endothelial PAS domain-containing protein 1
 UniProt Protein Name    Endothelial PAS domain-containing protein 1
 UniProt Synonym Protein Names   
Hypoxia-inducible factor 2-alpha; HIF-2-alpha; HIF2-alpha
 UniProt Gene Name    Epas1 [Similar Products]
 UniProt Synonym Gene Names    Hif2a; EPAS-1; HIF-2-alpha; HIF2-alpha [Similar Products]
 UniProt Entry Name    EPAS1_RAT
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 NCBI Summary for HIF2a    activates luciferase activity after transfection [RGD, Feb 2006]
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 UniProt Comments for HIF2a    Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD ().
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 Research Articles on HIF2a    1. Results showed that the inhibitory effects of TNFa on the hypoxia-induced upregulation of erythropoietin expression are mediated primarily by HIF-2a rather than HIF-1a
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HIF2a elisa kitPathways associated with HIF2a elisa kit
 Reference Product  PubMed Publications
 Egln1 elisa kit  >19 publications with HIF2a and Egln1
 Vhl elisa kit  >14 publications with HIF2a and Vhl
 Arnt elisa kit  >10 publications with HIF2a and Arnt
 Vegfa elisa kit  >9 publications with HIF2a and Vegfa
 Epo elisa kit  >5 publications with HIF2a and Epo
 Car9 elisa kit  >1 publications with HIF2a and Car9
 Egln3 elisa kit  >1 publications with HIF2a and Egln3
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Cellular Response To Hypoxia Pathway antibodies  Cellular Response To Hypoxia Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Oxygen-dependent Asparagine Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway antibodies  Oxygen-dependent Asparagine Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway Diagram
 Oxygen-dependent Proline Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway antibodies  Oxygen-dependent Proline Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram
 Regulation Of Hypoxia-inducible Factor (HIF) By Oxygen Pathway antibodies  Regulation Of Hypoxia-inducible Factor (HIF) By Oxygen Pathway Diagram
 Regulation Of Gene Expression By Hypoxia-inducible Factor Pathway antibodies  Regulation Of Gene Expression By Hypoxia-inducible Factor Pathway Diagram
 Renal Cell Carcinoma Pathway antibodies  Renal Cell Carcinoma Pathway Diagram
 Renal Cell Carcinoma Pathway antibodies  Renal Cell Carcinoma Pathway Diagram
Diseases associated with HIF2a elisa kitOrgans/Tissues associated with HIF2a elisa kit
 Disease Name  Pubmed Publications
 Carcinoma, Renal Cell Antibodies  >10 publications with HIF2a and Carcinoma, Renal Cell
 Disease Models, Animal Antibodies  >8 publications with HIF2a and Disease Models, Animal
 Kidney Diseases Antibodies  >7 publications with HIF2a and Kidney Diseases
 Inflammation Antibodies  >6 publications with HIF2a and Inflammation
 Necrosis Antibodies  >6 publications with HIF2a and Necrosis
 Neoplasms, Experimental Antibodies  >4 publications with HIF2a and Neoplasms, Experimental
 Lung Diseases Antibodies  >4 publications with HIF2a and Lung Diseases
 Hypertrophy Antibodies  >4 publications with HIF2a and Hypertrophy
 Carcinoma, Hepatocellular Antibodies  >4 publications with HIF2a and Carcinoma, Hepatocellular
 Liver Diseases Antibodies  >3 publications with HIF2a and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >14 publications with HIF2a and Kidney
 Heart Antibodies  >13 publications with HIF2a and Heart
 Lung Antibodies  >12 publications with HIF2a and Lung
 Embryonic Tissue Antibodies  >9 publications with HIF2a and Embryonic Tissue
 Adrenal Gland Antibodies  >7 publications with HIF2a and Adrenal Gland
 Connective Tissue Antibodies  >6 publications with HIF2a and Connective Tissue
 Brain Antibodies  >6 publications with HIF2a and Brain
 Placenta Antibodies  >6 publications with HIF2a and Placenta
 Colon Antibodies  >2 publications with HIF2a and Colon
 Eye Antibodies  >2 publications with HIF2a and Eye
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