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IGFBP-3 elisa kit :: Rat IGFBP-3 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2515458
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

IGFBP-3, ELISA Kit

★Popular Item★
 Also Known As   

Rat IGFBP-3 (Insulin-Like Growth Factor Binding Protein 3) ELISA Kit

 Product Gene Name   

IGFBP-3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2515458 COA    COA PDF
 MBS2515458 QC Report    QC Report PDF
 OMIM    146732
 3D Structure    ModBase 3D Structure for P17936
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 Species Reactivity    Rat
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 Specificity    This kit recognizes natural and some recombinant Rat IGFBP-3. No significant crossreactivity or interference between Rat IGFBP-3 and analogues was observed.
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 Samples    Serum, plasma and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    78.13-5000 pg/mL
 Sensitivity    46.88 pg/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IGFBP-3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IGFBP-3 purchase    MBS2515458 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IGFBP-3, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 78.13-5000 pg/mL in biological research samples containing IGFBP-3, with an estimated sensitivity of 46.88 pg/mL. The ELISA analytical biochemical technique of the MBS2515458 kit is based on IGFBP-3 antibody-IGFBP-3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IGFBP-3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IGFBP-3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IGFBP-3 elisa kit

   Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Rat IGFBP-3 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Rat IGFBP-3. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Rat IGFBP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Rat IGFBP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Rat IGFBP-3. The concentration of Rat IGFBP-3 in samples can be calculated by comparing the OD of the samples with the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of IGFBP-3 elisa kit    IGFBP-3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2515458. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IGFBP-3. It may not necessarily be applicable to this product.
 NCBI GI #    62243068
 NCBI GeneID    3486
 NCBI Accession #    NP_000589.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000598.4 [Other Products]
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 UniProt Primary Accession #    P17936 [Other Products]
 UniProt Secondary Accession #    Q2V509; Q6P1M6; Q9UCL4; A4D2F5; D3DVM0 [Other Products]
 UniProt Related Accession #    P17936 [Other Products]
 Molecular Weight    32,223 Da
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 NCBI Official Full Name    insulin-like growth factor-binding protein 3 isoform b
 NCBI Official Synonym Full Names    insulin-like growth factor binding protein 3
 NCBI Official Symbol    IGFBP3 [Similar Products]
 NCBI Official Synonym Symbols   
IBP3; BP-53
[Similar Products]
 NCBI Protein Information    insulin-like growth factor-binding protein 3; IBP-3; IGF-binding protein 3; IGFBP-3; acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; growth hormone-dependent binding protein
 UniProt Protein Name    Insulin-like growth factor-binding protein 3
 UniProt Gene Name    IGFBP3 [Similar Products]
 UniProt Synonym Gene Names    IBP3; IBP-3; IGF-binding protein 3; IGFBP-3 [Similar Products]
 UniProt Entry Name    IBP3_HUMAN
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 NCBI Summary for IGFBP-3    This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
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 UniProt Comments for IGFBP-3    IGFBP3: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.

Protein type: Secreted, signal peptide; Cell development/differentiation; Secreted

Chromosomal Location of Human Ortholog: 7p12.3

Cellular Component: insulin-like growth factor binding protein complex; extracellular space; extracellular region; nucleus

Molecular Function: insulin-like growth factor binding; protein binding; insulin-like growth factor I binding; metal ion binding; fibronectin binding; insulin-like growth factor II binding; protein tyrosine phosphatase activator activity

Biological Process: positive regulation of catalytic activity; positive regulation of insulin-like growth factor receptor signaling pathway; regulation of insulin-like growth factor receptor signaling pathway; positive regulation of apoptosis; apoptosis; negative regulation of smooth muscle cell proliferation; negative regulation of smooth muscle cell migration; protein amino acid phosphorylation; positive regulation of myoblast differentiation; negative regulation of signal transduction; osteoblast differentiation; negative regulation of cell proliferation; cellular protein metabolic process; negative regulation of protein amino acid phosphorylation; positive regulation of MAPKKK cascade; regulation of cell growth
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 Research Articles on IGFBP-3    1. Data indicate insulin-like growth factor (IGF)-binding protein-3 (IGFBP3) as a miR-21 target gene in glioblastoma and suggest that the oncogenic miRNA miR-21 down-regulates the expression of IGFBP3, which acts as a tumor suppressor in human glioblastoma.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IGFBP-3 elisa kitPathways associated with IGFBP-3 elisa kit
 Reference Product  PubMed Publications
 IGF1 elisa kit  >221 publications with IGFBP-3 and IGF1
 IGF2 elisa kit  >75 publications with IGFBP-3 and IGF2
 IGFALS elisa kit  >20 publications with IGFBP-3 and IGFALS
 TP53 elisa kit  >12 publications with IGFBP-3 and TP53
 MMP2 elisa kit  >5 publications with IGFBP-3 and MMP2
 ADAM12 elisa kit  >2 publications with IGFBP-3 and ADAM12
 FN1 elisa kit  >1 publications with IGFBP-3 and FN1
 Products by Pathway  Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Myometrial Relaxation And Contraction Pathways antibodies  Myometrial Relaxation And Contraction Pathways Diagram
 Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway antibodies  Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway Diagram
 Senescence And Autophagy Pathway antibodies  Senescence And Autophagy Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 P53 Signaling Pathway antibodies  P53 Signaling Pathway Diagram
 P53 Signaling Pathway antibodies  P53 Signaling Pathway Diagram
Diseases associated with IGFBP-3 elisa kitOrgans/Tissues associated with IGFBP-3 elisa kit
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >350 publications with IGFBP-3 and Breast Neoplasms
 Prostatic Neoplasms Antibodies  >262 publications with IGFBP-3 and Prostatic Neoplasms
 Cardiovascular Diseases Antibodies  >229 publications with IGFBP-3 and Cardiovascular Diseases
 Adenocarcinoma Antibodies  >175 publications with IGFBP-3 and Adenocarcinoma
 Liver Diseases Antibodies  >150 publications with IGFBP-3 and Liver Diseases
 Colorectal Neoplasms Antibodies  >149 publications with IGFBP-3 and Colorectal Neoplasms
 Weight Gain Antibodies  >145 publications with IGFBP-3 and Weight Gain
 Lung Diseases Antibodies  >139 publications with IGFBP-3 and Lung Diseases
 Inflammation Antibodies  >131 publications with IGFBP-3 and Inflammation
 Kidney Diseases Antibodies  >123 publications with IGFBP-3 and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2848 publications with IGFBP-3 and Blood
 Bone Antibodies  >517 publications with IGFBP-3 and Bone
 Liver Antibodies  >429 publications with IGFBP-3 and Liver
 Prostate Antibodies  >355 publications with IGFBP-3 and Prostate
 Muscle Antibodies  >297 publications with IGFBP-3 and Muscle
 Vascular Antibodies  >225 publications with IGFBP-3 and Vascular
 Connective Tissue Antibodies  >219 publications with IGFBP-3 and Connective Tissue
 Brain Antibodies  >204 publications with IGFBP-3 and Brain
 Kidney Antibodies  >202 publications with IGFBP-3 and Kidney
 Lung Antibodies  >187 publications with IGFBP-3 and Lung
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