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IL-25 elisa kit :: Rat IL-25 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2515787
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

IL-25, ELISA Kit

★Popular Item★
 Also Known As   

Rat IL-25 (Interleukin 25) ELISA Kit

 Product Gene Name   

IL-25 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    605658
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 Species Reactivity    Rat
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 Specificity    This kit recognizes natural and recombinant Rat IL-25. No significant cross-reactivity or interference between Rat IL-25 and analogues was observed.
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 Samples    Serum, plasma and other biological fluids
 Assay Type    Sandwich
 Detection Range    15.625-1000pg/mL
 Sensitivity    The minimum detectable dose of Rat IL-25 is 9.375pg/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
 Intended Uses    This ELISA kit applies to the in vitro quantitative determination of Rat IL-25 concentrations in serum, plasma and other biological fluids.
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL-25 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL-25 purchase    MBS2515787 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IL-25, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.625-1000pg/mL in biological research samples containing IL-25, with an estimated sensitivity of The minimum detectable dose of Rat IL-25 is 9.375pg/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero). The ELISA analytical biochemical technique of the MBS2515787 kit is based on IL-25 antibody-IL-25 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-25 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-25. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL-25 elisa kit

   Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-25. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-25 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-25, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IL-25. You can calculate the concentration of IL-25 in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of IL-25 elisa kit    IL-25 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2515787. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL-25. It may not necessarily be applicable to this product.
 NCBI GI #    27477081
 NCBI GeneID    64806
 NCBI Accession #    NP_758525.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_172314.1 [Other Products]
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 UniProt Secondary Accession #    Q2M3F0; Q8IZV3; Q8WXB0 [Other Products]
 UniProt Related Accession #    Q9H293 [Other Products]
 Molecular Weight    18,523 Da
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 NCBI Official Full Name    interleukin-25 isoform 2
 NCBI Official Synonym Full Names    interleukin 25
 NCBI Official Symbol    IL25 [Similar Products]
 NCBI Official Synonym Symbols   
IL17E
[Similar Products]
 NCBI Protein Information    interleukin-25; interleukin-17E
 UniProt Protein Name    Interleukin-25
 UniProt Synonym Protein Names   
Interleukin-17E; IL-17E
 UniProt Gene Name    IL25 [Similar Products]
 UniProt Synonym Gene Names    IL17E; IL-25; IL-17E [Similar Products]
 UniProt Entry Name    IL25_HUMAN
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 NCBI Summary for IL-25    The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
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 UniProt Comments for IL-25    IL25: Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses. Belongs to the IL-17 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular space

Molecular Function: cytokine activity; interleukin-17E receptor binding

Biological Process: response to fungus; inflammatory response to antigenic stimulus; response to nematode; eosinophil differentiation; positive regulation of transcription from RNA polymerase II promoter
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 Research Articles on IL-25    1. Because IL-25 has a major role in triggering type 2 responses, bronchial epithelial IL-25 expression is likely a key determinant of type 2 response activation in asthma
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL-25 elisa kitPathways associated with IL-25 elisa kit
 Reference Product  PubMed Publications
 IL33 elisa kit  >14 publications with IL-25 and IL33
 IL17RB elisa kit  >3 publications with IL-25 and IL17RB
 ITGAX elisa kit  >2 publications with IL-25 and ITGAX
 IL23A elisa kit  >1 publications with IL-25 and IL23A
 CCL11 elisa kit  >1 publications with IL-25 and CCL11
 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 IL17 Signaling Pathway antibodies  IL17 Signaling Pathway Diagram
Diseases associated with IL-25 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >33 publications with IL-25 and Inflammation
 Hypersensitivity Antibodies  >28 publications with IL-25 and Hypersensitivity
 Lung Diseases Antibodies  >19 publications with IL-25 and Lung Diseases
 Respiratory Hypersensitivity Antibodies  >14 publications with IL-25 and Respiratory Hypersensitivity
 Hyperplasia Antibodies  >7 publications with IL-25 and Hyperplasia
 Dermatitis, Atopic Antibodies  >7 publications with IL-25 and Dermatitis, Atopic
 Necrosis Antibodies  >4 publications with IL-25 and Necrosis
 Colitis Antibodies  >4 publications with IL-25 and Colitis
 Liver Diseases Antibodies  >2 publications with IL-25 and Liver Diseases
 Death Antibodies  >2 publications with IL-25 and Death
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