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IL-27 elisa kit :: Rat IL-27 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2515660
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
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 Product Name   

IL-27, ELISA Kit

★Popular Item★
 Also Known As   

Rat IL-27 (Interleukin 27) ELISA Kit

 Product Gene Name   

IL-27 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    608273
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 Species Reactivity    Rat
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 Specificity    This kit recognizes natural and recombinantRatIL-27. No significant cross-reactivity or interference between RatIL-27 and analogues was observed.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    15.625-1000pg/mL
 Sensitivity    Min: 9.375pg/mL; Max: 1000pg/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL-27 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL-27 purchase    MBS2515660 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IL-27, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.625-1000pg/mL in biological research samples containing IL-27, with an estimated sensitivity of Min: 9.375pg/mL; Max: 1000pg/mL. The ELISA analytical biochemical technique of the MBS2515660 kit is based on IL-27 antibody-IL-27 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-27 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-27. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL-27 elisa kit

   Intended Uses: This ELISA kit applies to the invitro quantitative determination of RatIL-27concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-27. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-27and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-27, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IL-27.You can calculate the concentration of IL-27in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of IL-27 elisa kit    IL-27 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2515660. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL-27. It may not necessarily be applicable to this product.
 NCBI GI #    28416913
 NCBI GeneID    246778
 NCBI Accession #    NP_663634.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_145659.3 [Other Products]
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 UniProt Secondary Accession #    Q6P676; A0N0L2 [Other Products]
 UniProt Related Accession #    Q8NEV9 [Other Products]
 Molecular Weight    27,493 Da
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 NCBI Official Full Name    interleukin-27 subunit alpha
 NCBI Official Synonym Full Names    interleukin 27
 NCBI Official Symbol    IL27 [Similar Products]
 NCBI Official Synonym Symbols   
p28; IL30; IL-27; IL27A; IL-27A; IL27p28
[Similar Products]
 NCBI Protein Information    interleukin-27 subunit alpha; IL-27 p28 subunit; IL-27 subunit alpha; IL-27-A; IL27-A; interleukin 30; interleukin-30
 UniProt Protein Name    Interleukin-27 subunit alpha
 UniProt Synonym Protein Names   
Interleukin-30; p28
 UniProt Gene Name    IL27 [Similar Products]
 UniProt Synonym Gene Names    IL27A; IL30; IL-27 subunit alpha; IL-27-A; IL27-A [Similar Products]
 UniProt Entry Name    IL27A_HUMAN
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 NCBI Summary for IL-27    The protein encoded by this gene is one of the subunits of a heterodimeric cytokine complex. This protein is related to interleukin 12A (IL12A). It interacts with Epstein-Barr virus induced gene 3 (EBI3), a protein similar to interleukin 12B (IL12B), and forms a complex that has been shown to drive rapid expansion of naive but not memory CD4(+) T cells. The complex is also found to synergize strongly with interleukin 12 to trigger interferon gamma (IFNG) production of naive CD4(+) T cells. The biological effects of this cytokine are mediated by the class I cytokine receptor (WSX1/TCRR). [provided by RefSeq, Jul 2008]
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 UniProt Comments for IL-27    IL27-alpha: Cytokine with pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon- gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK- dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL-27 subunit p28 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T-cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP- 10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increase the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replivation. Belongs to the IL-6 superfamily.

Protein type: Secreted, signal peptide; Secreted; Cytokine

Chromosomal Location of Human Ortholog: 16p11

Cellular Component: extracellular space

Molecular Function: interleukin-27 receptor binding; cytokine activity; receptor binding

Biological Process: regulation of T-helper 1 cell differentiation; response to bacterium; regulation of defense response to virus; regulation of T cell proliferation; innate immune response; inflammatory response; positive regulation of interferon-gamma biosynthetic process
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 Research Articles on IL-27    1. plasma IL-27 level was significantly upregulated in HIV-mono-infected and HIV/HCV-co-infected Chinese
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL-27 elisa kitPathways associated with IL-27 elisa kit
 Reference Product  PubMed Publications
 EBI3 elisa kit  >75 publications with IL-27 and EBI3
 IL27RA elisa kit  >66 publications with IL-27 and IL27RA
 TBX21 elisa kit  >20 publications with IL-27 and TBX21
 IL23A elisa kit  >13 publications with IL-27 and IL23A
 IL6ST elisa kit  >8 publications with IL-27 and IL6ST
 IL23R elisa kit  >2 publications with IL-27 and IL23R
 DMBT1 elisa kit  >2 publications with IL-27 and DMBT1
 STAT6 elisa kit  >2 publications with IL-27 and STAT6
 IL12RB2 elisa kit  >1 publications with IL-27 and IL12RB2
 IDO1 elisa kit  >1 publications with IL-27 and IDO1
 Products by Pathway  Pathway Diagram
 IL27-mediated Signaling Events Pathway antibodies  IL27-mediated Signaling Events Pathway Diagram
Diseases associated with IL-27 elisa kitOrgans/Tissues associated with IL-27 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >162 publications with IL-27 and Inflammation
 Necrosis Antibodies  >49 publications with IL-27 and Necrosis
 Encephalomyelitis, Autoimmune, Experimental Antibodies  >32 publications with IL-27 and Encephalomyelitis, Autoimmune, Experimental
 Liver Diseases Antibodies  >21 publications with IL-27 and Liver Diseases
 Colitis Antibodies  >17 publications with IL-27 and Colitis
 Arthritis, Experimental Antibodies  >13 publications with IL-27 and Arthritis, Experimental
 Death Antibodies  >10 publications with IL-27 and Death
 Heart Diseases Antibodies  >8 publications with IL-27 and Heart Diseases
 Kidney Diseases Antibodies  >8 publications with IL-27 and Kidney Diseases
 Diabetes Mellitus, Type 1 Antibodies  >7 publications with IL-27 and Diabetes Mellitus, Type 1
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >31 publications with IL-27 and Lung
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