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IL6 elisa kit :: Rat IL-6 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS824560
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Tests  /  $470 +1 FREE 8GB USB
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 Product Name   

IL-6 (IL6), ELISA Kit

 Also Known As   

Rat IL-6 ELISA Kit

 Product Synonym Names    IFNB2; Interleukin-6; IL-6; B-cell stimulatory factor 2; BSF-2; CTL differentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2
 Product Gene Name   

IL6 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 3D Structure    ModBase 3D Structure for P20607
 Species Reactivity    Rat
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 Specificity    The Rat IL-6 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat IL-6 proteins within the range of 31.3 pg/ml - 2000 pg/ml.
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 Samples    Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates.
 Sensitivity    60 pg/ml.
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 Preparation and Storage    Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of IL6 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL6 purchase    MBS824560 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IL-6 (IL6) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL6, with an estimated sensitivity of 60 pg/ml. The ELISA analytical biochemical technique of the MBS824560 kit is based on IL6 antibody-IL6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL6 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL6. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for IL6 elisa kit

   Principle of the Assay: The Rat IL-6 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-6 in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Rat IL-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Interleukin 6 is a multifunctional cytokine that plays important roles in host defense, acute phase reactions, immune responses, nerve cell functions and hematopoiesis. IL-6 is a prototypic member of the IL-6 superfamily of cytokines that share gp130 as a component required for signal transduction. The rat, mouse and human IL-6 cDNAs have been cloned. Rat IL-6 cDNA encodes a 211 amino acid residue precursor polypeptide with a hydrophobic signal peptide that is cleaved to generate the 187 aa residue mature protein. At the protein sequence level, there is approximately 39% identity between rat and human, and 87% identity between mouse and rat IL-6. IL-6 is expressed by a variety of normal and transformed lymphoid and non-lymphoid cells. The production of IL-6 is up-regulated by numerous signals such as mitogenic or antigenic stimulation, lipopolysaccharides, calcium ionophores, cytokines and viruses. IL-4, IL-10 and IL-13 inhibit IL-6 expression in monocytes. Elevated serum IL-6 levels have been observed in a number of pathological conditions, including bacterial and viral infections,trauma, autoimmune diseases, inflammations and malignancies.
 Applications Tested/Suitable for IL6 elisa kit   

Sandwich ELISA (SE)

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 Typical Testing Data/Standard Curve (for reference only) of IL6 elisa kit    IL6 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS824560
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NCBI/Uniprot data below describe general gene information for IL6. It may not necessarily be applicable to this product.
 NCBI GI #    7549769
 NCBI GeneID    24498
 NCBI Accession #    NP_036721.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_012589.2 [Other Products]
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 UniProt Primary Accession #    P20607 [Other Products]
 UniProt Related Accession #    P20607 [Other Products]
 Molecular Weight    24,357 Da
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 NCBI Official Full Name    interleukin-6
 NCBI Official Synonym Full Names    interleukin 6
 NCBI Official Symbol    Il6 [Similar Products]
 NCBI Official Synonym Symbols   
ILg6; Ifnb2
[Similar Products]
 NCBI Protein Information    interleukin-6; IL-6; Interleukin 6 (interferon, beta 2)
 UniProt Protein Name    Interleukin-6
 Protein Family    Interleukin
 UniProt Gene Name    Il6 [Similar Products]
 UniProt Synonym Gene Names    Il-6; IL-6 [Similar Products]
 UniProt Entry Name    IL6_RAT
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 NCBI Summary for IL6    a cytokine involved in development and possibly in neurodegenerative processes [RGD, Feb 2006]
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 UniProt Comments for IL6    Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation ().
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 Research Articles on IL6    1. The findings of the present study suggest that part of ischemic injury in the embolic stroke may be mediated through the increased levels of inflammatory cytokines.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL6 elisa kitPathways associated with IL6 elisa kit
 Reference Product  PubMed Publications
 Tnf elisa kit  >1392 publications with IL6 and Tnf
 Crp elisa kit  >463 publications with IL6 and Crp
 Stat3 elisa kit  >366 publications with IL6 and Stat3
 Icam1 elisa kit  >148 publications with IL6 and Icam1
 Rela elisa kit  >61 publications with IL6 and Rela
 Il6st elisa kit  >46 publications with IL6 and Il6st
 Fos elisa kit  >42 publications with IL6 and Fos
 Il12b elisa kit  >38 publications with IL6 and Il12b
 Nfkb1 elisa kit  >29 publications with IL6 and Nfkb1
 Il6ra elisa kit  >1 publications with IL6 and Il6ra
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Diseases associated with IL6 elisa kitOrgans/Tissues associated with IL6 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >2270 publications with IL6 and Inflammation
 Necrosis Antibodies  >1642 publications with IL6 and Necrosis
 Autoimmune Diseases Antibodies  >431 publications with IL6 and Autoimmune Diseases
 Skin Diseases Antibodies  >279 publications with IL6 and Skin Diseases
 Arthritis, Rheumatoid Antibodies  >260 publications with IL6 and Arthritis, Rheumatoid
 Atherosclerosis Antibodies  >230 publications with IL6 and Atherosclerosis
 Myocardial Ischemia Antibodies  >221 publications with IL6 and Myocardial Ischemia
 Ischemia Antibodies  >118 publications with IL6 and Ischemia
 Diabetes Mellitus, Type 2 Antibodies  >103 publications with IL6 and Diabetes Mellitus, Type 2
 Breast Neoplasms Antibodies  >100 publications with IL6 and Breast Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Uncharacterized Tissue Antibodies  >1 publications with IL6 and Uncharacterized Tissue
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