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IL-6 elisa kit :: Rat IL-6 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS355410
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Tests  /  $410 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

IL-6, ELISA Kit

★Popular Item★
 Also Known As   

IL-6 Rat ELISA Kit

 Product Synonym Names    Rat IL-6
 Product Gene Name   

IL-6 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 4; NC_005103.3 (3095536..3100112). Location: 4q11
 3D Structure    ModBase 3D Structure for P20607
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 Species Reactivity    Rat
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 Samples    rat serum, body fluids, tissue lysates or cell culture supernates
 Assay Type    Quantitative Sandwich
 Detection Range    62.5 pg/ml-4000 pg/ml
 Sensitivity    < 5 pg/ml
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 Preparation and Storage    Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL-6 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL-6 purchase    MBS355410 is a ready-to-use microwell, strip-or-full plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IL-6, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5 pg/ml-4000 pg/ml in biological research samples containing IL-6, with an estimated sensitivity of < 5 pg/ml. The ELISA analytical biochemical technique of the MBS355410 kit is based on IL-6 antibody-IL-6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-6 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-6. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as rat serum, body fluids, tissue lysates or cell culture supernates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL-6 elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-6 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-6 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-6 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-6 can be calculated.

Background: Interleukin-6 (IL-6) is an immunoregulatory cytokine that activates a cell surface signaling assembly composed of IL6, IL6RA, and the shared signaling receptor gp130. It acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 is one of the most important mediators of fever and of the acute phase response. It is capable of crossing the blood brain barrier and initiating synthesis of PGE2 in the hypothalamus, thereby changing the body's temperature setpoint. IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. Inhibitors of IL-6 are used to treat postmenopausal osteoporosis. IL-6 is relevant to many diseases such as diabetes, atherosclerosis, depression, Alzheimer's Disease, systemic lupus erythematosus, prostate cancer, and rheumatoid arthritis.
 Applications Tested/Suitable for IL-6 elisa kit   

Quantitative sELISA (EIA)

 Application Notes for IL-6 elisa kit    For quantitative detection of IL-6 in rat serum, body fluids, tissue lysates or cell culture supernates.
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  Typical Testing Data/Standard Curve (for reference only) of IL-6 elisa kit    IL-6 elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS355410. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL-6. It may not necessarily be applicable to this product.
 NCBI GI #    7549769
 NCBI GeneID    24498
 NCBI Accession #    NP_036721.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_012589.2 [Other Products]
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 UniProt Primary Accession #    P20607 [Other Products]
 UniProt Related Accession #    P20607 [Other Products]
 Molecular Weight    24,357 Da
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 NCBI Official Full Name    interleukin-6
 NCBI Official Synonym Full Names    interleukin 6
 NCBI Official Symbol    Il6 [Similar Products]
 NCBI Official Synonym Symbols   
ILg6; Ifnb2
[Similar Products]
 NCBI Protein Information    interleukin-6; IL-6; Interleukin 6 (interferon, beta 2)
 UniProt Protein Name    Interleukin-6
 UniProt Gene Name    Il6 [Similar Products]
 UniProt Synonym Gene Names    Il-6; IL-6 [Similar Products]
 UniProt Entry Name    IL6_RAT
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 NCBI Summary for IL-6    a cytokine involved in development and possibly in neurodegenerative processes [RGD, Feb 2006]
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 UniProt Comments for IL-6    Function: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance

By similarity.

Subcellular location: Secreted.

Sequence similarities: Belongs to the IL-6 superfamily.
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 Research Articles on IL-6    1. conclude that the IL-6/STAT1/STAT3 signaling pathway and its responsive genes after a single bout of resistance exercise are an important event regulating the SC pool and behavior involved in muscle hypertrophy
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL-6 elisa kitPathways associated with IL-6 elisa kit
 Reference Product  PubMed Publications
 Tnf elisa kit  >1154 publications with IL-6 and Tnf
 Crp elisa kit  >376 publications with IL-6 and Crp
 Stat3 elisa kit  >260 publications with IL-6 and Stat3
 Rela elisa kit  >46 publications with IL-6 and Rela
 Il6st elisa kit  >41 publications with IL-6 and Il6st
 Fos elisa kit  >37 publications with IL-6 and Fos
 Il12b elisa kit  >30 publications with IL-6 and Il12b
 Nfkb1 elisa kit  >24 publications with IL-6 and Nfkb1
 Csf2 elisa kit  >20 publications with IL-6 and Csf2
 Il6ra elisa kit  >1 publications with IL-6 and Il6ra
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
Diseases associated with IL-6 elisa kitOrgans/Tissues associated with IL-6 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >1778 publications with IL-6 and Inflammation
 Necrosis Antibodies  >1358 publications with IL-6 and Necrosis
 Autoimmune Diseases Antibodies  >355 publications with IL-6 and Autoimmune Diseases
 Arthritis, Rheumatoid Antibodies  >222 publications with IL-6 and Arthritis, Rheumatoid
 Atherosclerosis Antibodies  >192 publications with IL-6 and Atherosclerosis
 Myocardial Ischemia Antibodies  >188 publications with IL-6 and Myocardial Ischemia
 Ischemia Antibodies  >99 publications with IL-6 and Ischemia
 Myocardial Infarction Antibodies  >87 publications with IL-6 and Myocardial Infarction
 Hypersensitivity Antibodies  >85 publications with IL-6 and Hypersensitivity
 Diabetes Mellitus, Type 2 Antibodies  >83 publications with IL-6 and Diabetes Mellitus, Type 2
 Organ/Tissue Name  Pubmed Publications
 Uncharacterized Tissue Antibodies  >1 publications with IL-6 and Uncharacterized Tissue
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