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| Product Name
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Insulin Like Growth Factor Binding Protein 7 (IGFBP7), ELISA Kit ★Popular Item★
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| Also Known As
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Rat Insulin Like Growth Factor Binding Protein 7 (IGFBP7) ELISA Kit
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| Product Synonym Names
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FSTL2; IGFBP-7v; MAC25; PSF; TAF; IGFBP-rP1; MAC25 protein; Prostacyclin-stimulating factor; PGI2-stimulating factor; Tumor-derived adhesion factor
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| Sample Manual Insert
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| Request for Current Manual Insert
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| OMIM
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L19182 mRNA
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| Species Reactivity
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Rat
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| Specificity
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This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor Binding Protein 7 (IGFBP7).
No significant cross-reactivity or interference between Insulin Like Growth Factor Binding Protein 7 (IGFBP7) and analogues was observed.
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| Assay Type
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Double-antibody Sandwich
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| Samples
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Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
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| Detection Range
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62.5-4000pg/mL
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| Sensitivity
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< 26.4pg/mL
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| Application
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Enzyme-linked immunosorbent assay for Antigen Detection.
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| Intra-assay Precision (Precision within an assay)
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3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein 7 (IGFBP7) were tested 20 times on one plate, respectively.
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| Inter-assay Precision (Precision between assays)
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3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein 7 (IGFBP7) were tested on 3 different plates, 8 replicates in each plate.
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| CV(%) =
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SD/meanX100
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| Intra-Assay
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CV<10%
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| Inter-Assay
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CV<12%
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| Preparation and Storage
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
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| Product Note
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Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
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| Other Notes
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Small volumes of IGFBP7 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Searchable Terms for IGFBP7 purchase
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MBS2703574 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Insulin Like Growth Factor Binding Protein 7 (IGFBP7) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5-4000pg/mL in biological research samples containing IGFBP7, with an estimated sensitivity of < 26.4pg/mL. The ELISA analytical biochemical technique of the MBS2703574 kit is based on IGFBP7 antibody-IGFBP7 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IGFBP7 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IGFBP7. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor Binding Protein 7 (IGFBP7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor Binding Protein 7 (IGFBP7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor Binding Protein 7 (IGFBP7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Like Growth Factor Binding Protein 7 (IGFBP7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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| Typical Testing Data/Standard Curve (for reference only) of IGFBP7 elisa kit
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| Sample Manual Insert of MBS2703574. Click to request current manual
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| NCBI/Uniprot data below describe general gene information for IGFBP7. It may not necessarily be applicable to this product. |
| NCBI GI #
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42542649
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| NCBI GeneID
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3490
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| NCBI Accession #
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AAH66339.1
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[Other Products]
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| UniProt Secondary Accession #
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Q07822; Q53YE6; Q9UCA8; B4E1N2; B7Z9W7
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[Other Products]
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| UniProt Related Accession #
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Q16270
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[Other Products]
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| Molecular Weight
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28,860 Da
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| NCBI Official Full Name
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Insulin-like growth factor binding protein 7
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| NCBI Official Synonym Full Names
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insulin like growth factor binding protein 7
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| NCBI Official Symbol
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IGFBP7
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[Similar Products]
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| NCBI Official Synonym Symbols
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AGM; PSF; TAF; FSTL2; IBP-7; MAC25; IGFBP-7; RAMSVPS; IGFBP-7v; IGFBPRP1
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[Similar Products]
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| NCBI Protein Information
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insulin-like growth factor-binding protein 7
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| UniProt Protein Name
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Insulin-like growth factor-binding protein 7
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| UniProt Synonym Protein Names
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IGFBP-rP1; MAC25 protein; PGI2-stimulating factor; Prostacyclin-stimulating factor; Tumor-derived adhesion factor; TAF
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| Protein Family
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Insulin-like growth factor-binding protein
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| UniProt Gene Name
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IGFBP7
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[Similar Products]
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| UniProt Synonym Gene Names
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MAC25; PSF; IBP-7; IGF-binding protein 7; IGFBP-7; TAF
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[Similar Products]
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| NCBI Summary for IGFBP7
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This gene encodes a member of the insulin-like growth factor (IGF)-binding protein (IGFBP) family. IGFBPs bind IGFs with high affinity, and regulate IGF availability in body fluids and tissues and modulate IGF binding to its receptors. This protein binds IGF-I and IGF-II with relatively low affinity, and belongs to a subfamily of low-affinity IGFBPs. It also stimulates prostacyclin production and cell adhesion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and one variant has been associated with retinal arterial macroaneurysm (PMID:21835307). [provided by RefSeq, Dec 2011]
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| UniProt Comments for IGFBP7
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Binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion.
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| Research Articles on IGFBP7
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1. epithelial cells and leukocytes from the urinary sediment. CONCLUSION: The gene expression pattern of IGFBP7 and TIMP-2 from urinary sediment, which contains desquamated renal tubular epithelial cells, did not correlate with [IGFBP7]x[TIMP-2] protein, indicating that IGFBP7 and TIMP-2 measured in the NephroCheck(R) test originated predominantly from intact but stressed cells of the kidney itself
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with IGFBP7 elisa kit | Pathways associated with IGFBP7 elisa kit |
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| Diseases associated with IGFBP7 elisa kit | Organs/Tissues associated with IGFBP7 elisa kit |
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ELISA Kit 
