Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow IFNg rightarrow Rat IFNg  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Ammonium phosphate, tribasic trihydrate Biochemical
 • VEGF D Recombinant Protein
 • DNASE1 Antibody
 • Prothrombin (F2) Antibody
 • 5-LO ELISA Kit
 • FAP Antibody
 • HRH1 - Histamine H1 Receptor Antibody
 • GDI1 ELISA Kit
 • Hepatitis A Virus VP1-P2A (HAV VP1-P2A ) Recombinant Protein
 • P 38 Mitogen-Activated Protein Kinase (P38MAPK) ELISA Kit
 • Complement factor H-related protein 1 (CFHR1) ELISA Kit
 • ACE2 Blocking Peptide
 • CRIP1 Antibody
 • Cysteine Rich Protein 1, Intestinal (CRIP1) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

IFNg elisa kit :: Rat Interferon Gamma (IFNg) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS450417
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $270 +1 FREE 8GB USB
96-Strip-Wells  /  $340 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,480 +1 FREE 8GB USB
10x96-Strip-Wells  /  $2,870 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interferon Gamma (IFNg), ELISA Kit

★Popular Item★
 Also Known As   

Rat Interferon Gamma (IFNg) ELISA Kit

 Product Synonym Names    IFN-G; IFG; IFI; INFr; IFN, Immune Interferon
 Product Gene Name   

IFNg elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P01581
 Species Reactivity    Rat
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of IFNg. No significant cross-reactivity or interference between IFNg and analogues was observed.
Section Bar
 Samples    Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
 Assay Type    Sandwich
 Detection Range    15.625-1000pg/mL
 Sensitivity    5.3pg/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IFNg were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IFNg were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Section Bar
 Preparation and Storage    For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IFNg elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IFNg purchase    MBS450417 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interferon Gamma (IFNg) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.625-1000pg/mL in biological research samples containing IFNg, with an estimated sensitivity of 5.3pg/mL. The ELISA analytical biochemical technique of the MBS450417 kit is based on IFNg antibody-IFNg antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IFNg antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IFNg. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Product Description specifically for IFNg elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IFNg in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IFNg. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to IFNg. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IFNg, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IFNg in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of IFNg elisa kit    IFNg elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS450417. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for IFNg. It may not necessarily be applicable to this product.
 NCBI GI #    20302055
 NCBI GeneID    25712
 NCBI Accession #    NP_620235.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_138880.2 [Other Products]
Section Bar
 UniProt Primary Accession #    P01581 [Other Products]
 UniProt Related Accession #    P01581 [Other Products]
 Molecular Weight    17,918 Da
Table BarTOPTable Bar
 NCBI Official Full Name    interferon gamma
 NCBI Official Synonym Full Names    interferon gamma
 NCBI Official Symbol    Ifng [Similar Products]
 NCBI Official Synonym Symbols   
IFNG2
[Similar Products]
 NCBI Protein Information    interferon gamma
 UniProt Protein Name    Interferon gamma
 Protein Family    Interferon
 UniProt Gene Name    Ifng [Similar Products]
 UniProt Synonym Gene Names    IFN-gamma [Similar Products]
Section Bar
 NCBI Summary for IFNg    an immune molecule produced by T lymphocytes in response to mitogens or antigens [RGD, Feb 2006]
Section Bar
 UniProt Comments for IFNg    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Table BarTOPTable Bar
 Research Articles on IFNg    1. IL-1beta and IFNgamma upregulate the expression of TRAF2 in insulin-producing INS-1E cells and isolated rat pancreatic islets.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with IFNg elisa kitPathways associated with IFNg elisa kit
 Reference Product  PubMed Publications
 Tnf elisa kit  >236 publications with IFNg and Tnf
 Stat1 elisa kit  >58 publications with IFNg and Stat1
 Ifngr1 elisa kit  >38 publications with IFNg and Ifngr1
 Socs1 elisa kit  >12 publications with IFNg and Socs1
 Jak2 elisa kit  >7 publications with IFNg and Jak2
 Jak1 elisa kit  >6 publications with IFNg and Jak1
 Ifnar2 elisa kit  >3 publications with IFNg and Ifnar2
 Ptpn6 elisa kit  >3 publications with IFNg and Ptpn6
 Socs3 elisa kit  >1 publications with IFNg and Socs3
 Ptpn11 elisa kit  >1 publications with IFNg and Ptpn11
 Products by Pathway  Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Autophagy Pathway antibodies  Autophagy Pathway Diagram
 Autophagy Pathway antibodies  Autophagy Pathway Diagram
Diseases associated with IFNg elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >211 publications with IFNg and Necrosis
 Autoimmune Diseases Antibodies  >119 publications with IFNg and Autoimmune Diseases
 Respiratory Tract Diseases Antibodies  >109 publications with IFNg and Respiratory Tract Diseases
 Hepatitis Antibodies  >55 publications with IFNg and Hepatitis
 Lymphoproliferative Disorders Antibodies  >36 publications with IFNg and Lymphoproliferative Disorders
 HIV Infections Antibodies  >28 publications with IFNg and HIV Infections
 Colitis Antibodies  >26 publications with IFNg and Colitis
 Hepatitis C Antibodies  >23 publications with IFNg and Hepatitis C
 Diabetes Mellitus, Type 1 Antibodies  >21 publications with IFNg and Diabetes Mellitus, Type 1
 Arthritis, Rheumatoid Antibodies  >20 publications with IFNg and Arthritis, Rheumatoid
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions