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IL-12/P70 elisa kit :: Rat Interleukin 12, IL-12/P70 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS705214
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $450 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,615 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,940 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interleukin 12, IL-12/P70, ELISA Kit

★Popular Item★
 Also Known As   

Rat Interleukin 12, IL-12/P70 ELISA Kit

 Product Synonym Names    Rat Interleukin 12; IL-12/P70 ELISA KIT; Interleukin 12; IL-12/P70
 Product Gene Name   

IL-12/P70 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS705214 Testing Data    Testing Data PDF
 OMIM    161560
 3D Structure    ModBase 3D Structure for P29459
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 Species Reactivity    Rat
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 Samples    Serum, plasma, tissue homogenates
 Detection Range    3.12 pg/ml-200 pg/ml
 Sensitivity    0.78 pg/ml
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL-12/P70 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL-12/P70 purchase    MBS705214 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 12, IL-12/P70, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.12 pg/ml-200 pg/ml in biological research samples containing IL-12/P70, with an estimated sensitivity of 0.78 pg/ml. The ELISA analytical biochemical technique of the MBS705214 kit is based on IL-12/P70 antibody-IL-12/P70 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-12/P70 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-12/P70. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL-12/P70 elisa kit

   Introduction: Interleukin 12 (IL-12) is an interleukin that is naturally produced by dendritic cells, macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is composed of a bundle of four alpha helices. It is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). The active heterodimer, and a homodimer of p40 are formed following protein synthesis. IL-12 is involved in the differentiation of naive T cells into Th1 cells, which is important in resistance against pathogens. It is known as a T cell stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha) from T and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-gamma. T cells which produce IL-12 have a coreceptor, CD30, which is associated with IL-12 activity. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes. There also seems to be a link between IL-2 and the signal transduction of IL-12 in NK cells. IL-2 stimulates the expression of two IL-12 receptors, IL-12R-beta1 and IL-12R-beta2, maintaining the expression of a critical protein involved in IL-12 signaling in NK cells. Enhanced functional response is demonstrated by IFN-gamma production and killing of target cells. IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels. It does this by increasing production of interferon gamma, which in turn increases the production of a chemokine called inducible protein-10 (IP-10 or CXCL10). IP-10 then mediates this anti-angiogenic effect. Because of its ability to induce immune responses and its anti-angiogenic activity, there has been an interest in testing IL-12 as a possible anti-cancer drug. However, it has not been shown to have substantial activity in the tumors tested to this date. There is a link that may be useful in treatment between IL-12 and the diseases psoriasis and inflammatory bowel disease.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-12/P70. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IL-12/P70 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IL-12/P70, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IL-12/P70 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of IL-12/P70 elisa kit    IL-12/P70 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS705214. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL-12/P70. It may not necessarily be applicable to this product.
 NCBI GI #    4323579
 NCBI GeneID    3592
 NCBI Accession #    AAD16432.1 [Other Products]
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 UniProt Primary Accession #    P29459 [Other Products]
 UniProt Secondary Accession #    Q96QZ1 [Other Products]
 UniProt Related Accession #    P29459 [Other Products]
 Molecular Weight    24,874 Da
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 NCBI Official Full Name    interleukin 12
 NCBI Official Synonym Full Names    interleukin 12A
 NCBI Official Symbol    IL12A [Similar Products]
 NCBI Official Synonym Symbols   
P35; CLMF; NFSK; NKSF1; IL-12A
[Similar Products]
 NCBI Protein Information    interleukin-12 subunit alpha; CLMF p35; IL35 subunit; IL-12, subunit p35; interleukin 12, p35; interleukin-12 alpha chain; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
 UniProt Protein Name    Interleukin-12 subunit alpha
 UniProt Synonym Protein Names   
Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; NK cell stimulatory factor chain 1; NKSF1
 UniProt Gene Name    IL12A [Similar Products]
 UniProt Synonym Gene Names    NKSF1; IL-12A; CLMF p35; NKSF1 [Similar Products]
 UniProt Entry Name    IL12A_HUMAN
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 NCBI Summary for IL-12/P70    This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]
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 UniProt Comments for IL-12/P70    Function: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
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 Research Articles on IL-12/P70    1. circulating IL-35 in PDAC patients significantly increased, suggesting that regulating the expression of IL-35 may provide a new possible target for the treatment of PDAC patients, especially for the resectable ones.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL-12/P70 elisa kitPathways associated with IL-12/P70 elisa kit
 Reference Product  PubMed Publications
 IL12B elisa kit  >47 publications with IL-12/P70 and IL12B
 IL4 elisa kit  >18 publications with IL-12/P70 and IL4
 IFNG elisa kit  >15 publications with IL-12/P70 and IFNG
 IL12RB2 elisa kit  >12 publications with IL-12/P70 and IL12RB2
 IL1B elisa kit  >11 publications with IL-12/P70 and IL1B
 IL12RB1 elisa kit  >10 publications with IL-12/P70 and IL12RB1
 STAT1 elisa kit  >5 publications with IL-12/P70 and STAT1
 JAK2 elisa kit  >1 publications with IL-12/P70 and JAK2
 Products by Pathway  Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
Diseases associated with IL-12/P70 elisa kitOrgans/Tissues associated with IL-12/P70 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >18 publications with IL-12/P70 and Inflammation
 Necrosis Antibodies  >16 publications with IL-12/P70 and Necrosis
 Liver Diseases Antibodies  >11 publications with IL-12/P70 and Liver Diseases
 Disease Models, Animal Antibodies  >10 publications with IL-12/P70 and Disease Models, Animal
 Multiple Sclerosis Antibodies  >10 publications with IL-12/P70 and Multiple Sclerosis
 Hepatitis Antibodies  >7 publications with IL-12/P70 and Hepatitis
 Brain Diseases Antibodies  >7 publications with IL-12/P70 and Brain Diseases
 Liver Cirrhosis, Biliary Antibodies  >7 publications with IL-12/P70 and Liver Cirrhosis, Biliary
 Neoplasms, Experimental Antibodies  >5 publications with IL-12/P70 and Neoplasms, Experimental
 Cell Transformation, Neoplastic Antibodies  >4 publications with IL-12/P70 and Cell Transformation, Neoplastic
 Organ/Tissue Name  Pubmed Publications
 Spleen Antibodies  >4 publications with IL-12/P70 and Spleen
 Uterus Antibodies  >2 publications with IL-12/P70 and Uterus
 Brain Antibodies  >2 publications with IL-12/P70 and Brain
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