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IL13 elisa kit :: Rat Interleukin 13 (IL13) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2700977 (SPECIAL PROMOTIONAL PRICING for a limited time)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $220 +1 FREE 8GB USB
48-Strip-Wells  /  $350 +1 FREE 8GB USB
96-Strip-Wells  /  $470 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,960 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,625 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interleukin 13 (IL13), ELISA Kit

★Popular Item★
 Also Known As   

Rat Interleukin 13 (IL13) ELISA Kit

 Product Synonym Names    ALRH; BHR1; P600; IL-13; NC30
 Product Gene Name   

IL13 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    X69079 mRNA
 3D Structure    ModBase 3D Structure for P35225
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Interleukin 13 (IL13).
No significant cross-reactivity or interference between Interleukin 13 (IL13) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
 Detection Range    7.8-500pg/mL
 Sensitivity    < 2.9pg/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Interleukin 13 (IL13) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Interleukin 13 (IL13) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL13 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL13 purchase    MBS2700977 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 13 (IL13) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 7.8-500pg/mL in biological research samples containing IL13, with an estimated sensitivity of < 2.9pg/mL. The ELISA analytical biochemical technique of the MBS2700977 kit is based on IL13 antibody-IL13 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL13 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL13. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL13 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 13 (IL13). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 13 (IL13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 13 (IL13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 13 (IL13) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of IL13 elisa kit    IL13 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2700977. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL13. It may not necessarily be applicable to this product.
 NCBI GI #    26787978
 NCBI GeneID    3596
 NCBI Accession #    NP_002179.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_002188.2 [Other Products]
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 UniProt Primary Accession #    P35225 [Other Products]
 UniProt Secondary Accession #    O43644; Q4VB52; Q9UDC7 [Other Products]
 UniProt Related Accession #    P35225 [Other Products]
 Molecular Weight    15,816 Da
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 NCBI Official Full Name    interleukin-13 isoform 1
 NCBI Official Synonym Full Names    interleukin 13
 NCBI Official Symbol    IL13 [Similar Products]
 NCBI Official Synonym Symbols   
P600; IL-13
[Similar Products]
 NCBI Protein Information    interleukin-13
 UniProt Protein Name    Interleukin-13
 Protein Family    Interleukin
 UniProt Gene Name    IL13 [Similar Products]
 UniProt Synonym Gene Names    NC30; IL-13 [Similar Products]
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 NCBI Summary for IL13    This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4. [provided by RefSeq, Jul 2008]
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 UniProt Comments for IL13    Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages ().
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 Research Articles on IL13    1. These findings demonstrate that NF-kappaB-mediated transcriptional mechanisms are critically involved in the IL-1beta-mediated IL-17C induction, and that IL-13 negatively regulates this induction by suppressing NF-kappaB-based transcriptional activation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL13 elisa kitPathways associated with IL13 elisa kit
 Reference Product  PubMed Publications
 STAT6 elisa kit  >379 publications with IL13 and STAT6
 IL13RA1 elisa kit  >287 publications with IL13 and IL13RA1
 IL5 elisa kit  >86 publications with IL13 and IL5
 IL18 elisa kit  >83 publications with IL13 and IL18
 GATA3 elisa kit  >75 publications with IL13 and GATA3
 IL4R elisa kit  >56 publications with IL13 and IL4R
 TSLP elisa kit  >26 publications with IL13 and TSLP
 IL13RA2 elisa kit  >11 publications with IL13 and IL13RA2
 VCAM1 elisa kit  >5 publications with IL13 and VCAM1
 Products by Pathway  Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokines And Inflammatory Response Pathway antibodies  Cytokines And Inflammatory Response Pathway Diagram
 Fc Epsilon RI Signaling Pathway antibodies  Fc Epsilon RI Signaling Pathway Diagram
 Fc Epsilon RI Signaling Pathway antibodies  Fc Epsilon RI Signaling Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram
 IL12 Signaling Mediated By STAT4 Pathway antibodies  IL12 Signaling Mediated By STAT4 Pathway Diagram
 Inflammatory Bowel Disease Pathway antibodies  Inflammatory Bowel Disease Pathway Diagram
Diseases associated with IL13 elisa kitOrgans/Tissues associated with IL13 elisa kit
 Disease Name  Pubmed Publications
 Hypersensitivity Antibodies  >1222 publications with IL13 and Hypersensitivity
 Inflammation Antibodies  >1146 publications with IL13 and Inflammation
 Asthma Antibodies  >1136 publications with IL13 and Asthma
 Hypersensitivity, Immediate Antibodies  >1058 publications with IL13 and Hypersensitivity, Immediate
 Lung Diseases Antibodies  >1041 publications with IL13 and Lung Diseases
 Respiratory Hypersensitivity Antibodies  >882 publications with IL13 and Respiratory Hypersensitivity
 Necrosis Antibodies  >562 publications with IL13 and Necrosis
 Fibrosis Antibodies  >290 publications with IL13 and Fibrosis
 Bronchial Hyperreactivity Antibodies  >211 publications with IL13 and Bronchial Hyperreactivity
 Nervous System Diseases Antibodies  >176 publications with IL13 and Nervous System Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >160 publications with IL13 and Brain
 Bone Marrow Antibodies  >126 publications with IL13 and Bone Marrow
 Mixed Antibodies  >65 publications with IL13 and Mixed
 Testis Antibodies  >6 publications with IL13 and Testis
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