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IL-5 elisa kit :: Rat Interleukin 5 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS725873
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells-(Competitive)  /  $470 +1 FREE 8GB USB
48-Strip-Wells-(Sandwich)  /  $470 +1 FREE 8GB USB
96-Strip-Wells-(Competitive)  /  $675 +1 FREE 8GB USB
96-Strip-Wells-(Sandwich)  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells-(Competitive)  /  $2,850 +3 FREE 8GB USB
5x96-Strip-Wells-(Sandwich)  /  $2,850 +3 FREE 8GB USB
10x96-Strip-Wells-(Competitive)  /  $5,340 +6 FREE 8GB USB
10x96-Strip-Wells-(Sandwich)  /  $5,340 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interleukin 5 (IL-5), ELISA Kit

 Also Known As   

Rat Interleukin 5 ELISA Kit

 Product Gene Name   

IL-5 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of IL-5. No significant cross-reactivity or interference between IL-5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-5 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Assay Type    Competitive or Sandwich
 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Sensitivity    1.0 pg/mL.
 Intended Uses    This IL-5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat IL-5. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of IL-5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL-5 purchase    MBS725873 is a ready-to-use microwell, strip plate Competitive or Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 5 (IL-5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-5, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS725873 kit is based on IL-5 antibody-IL-5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for IL-5 elisa kit

  
Principle of the assay: IL-5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-5 antibody and an IL-5-HRP conjugate. The assay sample and buffer are incubated together with IL-5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-5 concentration since IL-5 from samples and IL-5-HRP conjugate compete for the anti-IL-5 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-5 from the sample, fewer sites are left to bind IL-5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-5 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for IL-5 elisa kit    Immunology
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 Typical Testing Data/Standard Curve (for reference only) of IL-5 elisa kit    IL-5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS725873
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NCBI/Uniprot data below describe general gene information for IL-5. It may not necessarily be applicable to this product.
 NCBI GI #    54019426
 NCBI GeneID    24497
 NCBI Accession #    NP_068606.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_021834.1 [Other Products]
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 UniProt Related Accession #    Q08125 [Other Products]
 Molecular Weight    15,207 Da
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 NCBI Official Full Name    interleukin-5
 NCBI Official Synonym Full Names    interleukin 5
 NCBI Official Symbol    Il5 [Similar Products]
 NCBI Protein Information    interleukin-5; TRF; IL-5; BCGF-II; B-cell growth factor II; T-cell replacing factor; cytotoxic T-lymphocyte inducer; eosinophil differentiation factor; Interleukin 5 (colony-stimulating factor eosinophil); Interleukin 5 (colony-stimulating factor, eosinophil)
 UniProt Protein Name    Interleukin-5
 UniProt Synonym Protein Names   
B-cell growth factor II; BCGF-II; Cytotoxic T-lymphocyte inducer; Eosinophil differentiation factor; T-cell replacing factor; TRF
 UniProt Gene Name    Il5 [Similar Products]
 UniProt Synonym Gene Names    Il-5; IL-5; BCGF-II; TRF [Similar Products]
 UniProt Entry Name    IL5_RAT
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 NCBI Summary for IL-5    cytokine with B-cell growth factor activity [RGD, Feb 2006]
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 UniProt Comments for IL-5    Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
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 Research Articles on IL-5    1. Treatment with intranasal interferon gamma can inhibit production of IL4 and IL5 in allergic rhinitis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL-5 elisa kitPathways associated with IL-5 elisa kit
 Reference Product  PubMed Publications
 Il4 elisa kit  >210 publications with IL-5 and Il4
 Il13 elisa kit  >110 publications with IL-5 and Il13
 Il2 elisa kit  >100 publications with IL-5 and Il2
 Il9 elisa kit  >24 publications with IL-5 and Il9
 Il5ra elisa kit  >24 publications with IL-5 and Il5ra
 Il7 elisa kit  >15 publications with IL-5 and Il7
 Il15 elisa kit  >13 publications with IL-5 and Il15
 Csf2 elisa kit  >10 publications with IL-5 and Csf2
 Csf2rb elisa kit  >5 publications with IL-5 and Csf2rb
 Jak2 elisa kit  >2 publications with IL-5 and Jak2
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 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokines And Inflammatory Response (BioCarta) Pathway antibodies  Cytokines And Inflammatory Response (BioCarta) Pathway Diagram
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