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IL-8 elisa kit :: Rat Interleukin 8 (IL-8) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS262974
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $275 +1 FREE 8GB USB
96-Strip-Wells  /  $435 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interleukin 8 (IL-8), ELISA Kit

★Popular Item★
 Also Known As   

Rat Interleukin 8 (IL-8) ELISA Kit

 Product Gene Name   

IL-8 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    146930
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 Species Reactivity    Rat
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 Specificity    No cross-reaction with other factors.
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 Samples    Serum, plasma or cell culture supernatant
 Assay Type    Quantitative Sandwich
 Detection Range    1000 pg/ml-15.6 pg/ml
 Sensitivity    5 pg/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL-8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL-8 purchase    MBS262974 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 8 (IL-8) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1000 pg/ml-15.6 pg/ml in biological research samples containing IL-8, with an estimated sensitivity of 5 pg/ml. The ELISA analytical biochemical technique of the MBS262974 kit is based on IL-8 antibody-IL-8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma or cell culture supernatant. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL-8 elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat IL-8 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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  Typical Testing Data/Standard Curve (for reference only) of IL-8 elisa kit    IL-8 elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS262974. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL-8. It may not necessarily be applicable to this product.
 NCBI GI #    10834978
 NCBI GeneID    3576
 NCBI Accession #    NP_000575.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000584.3 [Other Products]
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 UniProt Secondary Accession #    Q6FGF6; Q6LAE6; Q96RG6; Q9C077; Q9UCE1; Q9UCR8; Q9UCR9; Q9UCS0; B2R4L8 [Other Products]
 UniProt Related Accession #    P10145 [Other Products]
 Molecular Weight    11,338 Da
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 NCBI Official Full Name    interleukin-8
 NCBI Official Synonym Full Names    chemokine (C-X-C motif) ligand 8
 NCBI Official Symbol    CXCL8 [Similar Products]
 NCBI Official Synonym Symbols   
IL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
[Similar Products]
 NCBI Protein Information    interleukin-8; emoctakin; interleukin 8; T-cell chemotactic factor; neutrophil-activating peptide 1; beta-thromboglobulin-like protein; granulocyte chemotactic protein 1; tumor necrosis factor-induced gene 1; alveolar macrophage chemotactic factor I; monocyte-derived neutrophil chemotactic factor; monocyte-derived neutrophil-activating peptide; small inducible cytokine subfamily B, member 8; lymphocyte derived neutrophil activating peptide; lung giant cell carcinoma-derived chemotactic protein; beta endothelial cell-derived neutrophil activating peptide
 UniProt Protein Name    Interleukin-8
 UniProt Synonym Protein Names   
C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; Granulocyte chemotactic protein 1; GCP-1; Monocyte-derived neutrophil chemotactic factor; MDNCF; Monocyte-derived neutrophil-activating peptide; MONAP; Neutrophil-activating protein 1; NAP-1; Protein 3-10C; T-cell chemotactic factorCleaved into the following 7 chains:MDNCF-aAlternative name(s):GCP/IL-8 protein IV; IL8/NAP1 form I
 UniProt Gene Name    CXCL8 [Similar Products]
 UniProt Synonym Gene Names    IL8; IL-8; GCP-1; MDNCF; MONAP; NAP-1; LYNAP; NAF [Similar Products]
 UniProt Entry Name    IL8_HUMAN
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 NCBI Summary for IL-8    The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. [provided by RefSeq, Jul 2008]
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 UniProt Comments for IL-8    IL8: IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. Belongs to the intercrine alpha (chemokine CxC) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q13-q21

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; chemokine activity; interleukin-8 receptor binding

Biological Process: regulation of cell adhesion; neutrophil chemotaxis; neutrophil activation; unfolded protein response; negative regulation of G-protein coupled receptor protein signaling pathway; calcium-mediated signaling; chemotaxis; signal transduction; regulation of retroviral genome replication; induction of positive chemotaxis; G-protein coupled receptor protein signaling pathway; negative regulation of cell proliferation; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; response to molecule of bacterial origin; receptor internalization; immune response; angiogenesis; cell motility; cell cycle arrest; inflammatory response; embryonic gut development
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 Research Articles on IL-8    1. These results suggested that CagA is not required for Helicobacter pylori-induced activation of NF-kappaB and upregulation of IL-8 expression in gastric epithelial cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with IL-8 elisa kitDiseases associated with IL-8 elisa kit
 Products by Pathway  Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 Activation Of Genes By ATF4 Pathway antibodies  Activation Of Genes By ATF4 Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >657 publications with IL-8 and Inflammation
 Necrosis Antibodies  >385 publications with IL-8 and Necrosis
 Hypersensitivity Antibodies  >65 publications with IL-8 and Hypersensitivity
 Drug Toxicity Antibodies  >60 publications with IL-8 and Drug Toxicity
 Adenocarcinoma Antibodies  >48 publications with IL-8 and Adenocarcinoma
 Pulmonary Disease, Chronic Obstructive Antibodies  >47 publications with IL-8 and Pulmonary Disease, Chronic Obstructive
 Pneumonia Antibodies  >34 publications with IL-8 and Pneumonia
 Colorectal Neoplasms Antibodies  >30 publications with IL-8 and Colorectal Neoplasms
 Kidney Diseases Antibodies  >26 publications with IL-8 and Kidney Diseases
 Breast Neoplasms Antibodies  >25 publications with IL-8 and Breast Neoplasms
Organs/Tissues associated with IL-8 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >659 publications with IL-8 and Blood
 Lung Antibodies  >230 publications with IL-8 and Lung
 Vascular Antibodies  >195 publications with IL-8 and Vascular
 Intestine Antibodies  >86 publications with IL-8 and Intestine
 Skin Antibodies  >81 publications with IL-8 and Skin
 Liver Antibodies  >70 publications with IL-8 and Liver
 Heart Antibodies  >66 publications with IL-8 and Heart
 Bone Antibodies  >59 publications with IL-8 and Bone
 Muscle Antibodies  >58 publications with IL-8 and Muscle
 Brain Antibodies  >47 publications with IL-8 and Brain
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