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IFABP elisa kit :: Rat Intestinal fatty acid binding protein ELISA Kit

Scan QR to view Datasheet Catalog #    MBS727438
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Intestinal fatty acid binding protein (IFABP), ELISA Kit

★Popular Item★
 Also Known As   

Rat Intestinal fatty acid binding protein ELISA Kit

 Product Gene Name   

IFABP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Rat
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    0.1 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IFABP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IFABP purchase    MBS727438 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Intestinal fatty acid binding protein (IFABP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IFABP, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS727438 kit is based on IFABP antibody-IFABP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IFABP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IFABP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for IFABP elisa kit

   Intended Uses: This iFABP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat iFABP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: iFABP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-iFABP antibody and an iFABP-HRP conjugate. The assay sample and buffer are incubated together with iFABP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the iFABP concentration since iFABP from samples and iFABP-HRP conjugate compete for the anti-iFABP antibody binding site. Since the number of sites is limited, as more sites are occupied by iFABP from the sample, fewer sites are left to bind iFABP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The iFABP concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for IFABP elisa kit    Cardiovascular
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 Typical Testing Data/Standard Curve (for reference only) of IFABP elisa kit    IFABP elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS727438. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IFABP. It may not necessarily be applicable to this product.
 NCBI GI #    6007485
 NCBI GeneID    30708
 NCBI Accession #    AAF00925.1 [Other Products]
 Molecular Weight    15,060 Da
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 NCBI Official Full Name    intestinal fatty acid binding protein
 NCBI Official Synonym Full Names    fatty acid binding protein 2, intestinal
 NCBI Official Symbol    fabp2 [Similar Products]
 NCBI Official Synonym Symbols   
ifabp; I-FABP; zgc:92264
[Similar Products]
 NCBI Protein Information    fatty acid-binding protein, intestinal; I-FABP (FABPI); intestinal fatty acid binding pr; intestinal fatty acid binding protein 2
 UniProt Protein Name    Fabp2 protein
 UniProt Synonym Protein Names   
Fabp2 protein; Intestinal fatty acid binding protein; Intestinal fatty acid binding protein 2; Uncharacterized protein
 UniProt Gene Name    fabp2 [Similar Products]
 UniProt Synonym Gene Names    I-FABP (FABPI); IFABP [Similar Products]
 UniProt Entry Name    Q9PRH9_DANRE
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 Research Articles on IFABP    1. transcription of single copy fabp2 is modulated by dietary fatty acids and clofibrate in a tissue specific fashion.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with IFABP elisa kit
 Products by Pathway  Pathway Diagram
 PPAR Signaling Pathway antibodies  PPAR Signaling Pathway Diagram
 PPAR Signaling Pathway antibodies  PPAR Signaling Pathway Diagram
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