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Ki67P elisa kit :: Rat Ki-67 Protein (Ki67P) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2021528 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $405 +1 FREE 8GB USB
48-Strip-Wells  /  $605 +1 FREE 8GB USB
96-Strip-Wells  /  $820 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,375 +4 FREE 8GB USB
10x96-Strip-Wells  /  $6,125 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Ki-67 Protein (Ki67P), ELISA Kit

 Also Known As   

Ki-67 Protein (Ki67P) ELISA Kit

 Product Synonym Names    MKI67; KIA; Ki-67; Antigen Identified By Monoclonal Antibody Ki-67
 Product Gene Name   

Ki67P elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 3D Structure    ModBase 3D Structure for Q61769
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Ki-67 Protein (Ki67P).
No significant cross-reactivity or interference between Ki-67 Protein (Ki67P) and analogues was observed.
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 Samples    Tissue homogenates, Cell lysates and other biological fluids
 Assay Type    Sandwich
 Detection Range    0.313-20ng/mL
 Sensitivity    Typically less than 0.115ng/mL.
 Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ki-67 Protein (Ki67P) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ki-67 Protein (Ki67P) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
 Assay Procedure Summary    1. Prepare all reagents, samples and standards;
2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;
3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;
4. Aspirate and wash 3 times;
5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;
6. Aspirate and wash 5 times;
7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;
8. Add 50uL Stop Solution. Read at 450nm immediately.
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 Preparation and Storage    For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C.

For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of Ki67P elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Ki67P purchase    MBS2021528 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Ki-67 Protein (Ki67P) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.313-20ng/mL in biological research samples containing Ki67P, with an estimated sensitivity of Typically less than 0.115ng/mL. The ELISA analytical biochemical technique of the MBS2021528 kit is based on Ki67P antibody-Ki67P antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Ki67P antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Ki67P. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for Ki67P elisa kit

   Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ki-67 Protein (Ki67P). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ki-67 Protein (Ki67P). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ki-67 Protein (Ki67P), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ki-67 Protein (Ki67P) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of Ki67P elisa kit    Ki67P elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2021528
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NCBI/Uniprot data below describe general gene information for Ki67P. It may not necessarily be applicable to this product.
 NCBI GI #    1177528
 NCBI Accession #    CAA58026.1 [Other Products]
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 UniProt Primary Accession #    Q61769 [Other Products]
 Molecular Weight    324,429 Da
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 NCBI Official Full Name    Ki-67
 UniProt Protein Name    Ki-67 protein
 UniProt Synonym Protein Names   
Ki-67 protein
 UniProt Gene Name    Mki67 [Similar Products]
 UniProt Entry Name    Q61769_MOUSE
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 UniProt Comments for Ki67P    KI-67: a protein that may be a marker of proliferating cells, involved in chromatin compaction. Its expression is altered in many tumor types including osteosarcomas, histiocytomas, prostate, breast and esophageal cancers. Mutated in colon, cervical and lung cancers.

Protein type: Nucleolus; Cell cycle regulation

Cellular Component: membrane; cytoplasm; nucleolus; condensed chromosome; nucleus; chromosome, pericentric region

Molecular Function: protein C-terminus binding

Biological Process: meiosis; cell proliferation
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Ki67P elisa kitOrgans/Tissues associated with Ki67P elisa kit
 Reference Product  PubMed Publications
 Ccnd1 elisa kit  >533 publications with Ki67P and Ccnd1
 Birc5 elisa kit  >112 publications with Ki67P and Birc5
 Ccnb1 elisa kit  >93 publications with Ki67P and Ccnb1
 Akt1 elisa kit  >23 publications with Ki67P and Akt1
 Cdk1 elisa kit  >17 publications with Ki67P and Cdk1
 Ccnb2 elisa kit  >11 publications with Ki67P and Ccnb2
 Ccna2 elisa kit  >9 publications with Ki67P and Ccna2
 Prc1 elisa kit  >2 publications with Ki67P and Prc1
 Ncapg elisa kit  >1 publications with Ki67P and Ncapg
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >1375 publications with Ki67P and Brain
 Skin Antibodies  >956 publications with Ki67P and Skin
 Lung Antibodies  >735 publications with Ki67P and Lung
 Prostate Antibodies  >506 publications with Ki67P and Prostate
 Intestine Antibodies  >433 publications with Ki67P and Intestine
 Kidney Antibodies  >396 publications with Ki67P and Kidney
 Eye Antibodies  >291 publications with Ki67P and Eye
 Bone Marrow Antibodies  >271 publications with Ki67P and Bone Marrow
 Embryonic Tissue Antibodies  >252 publications with Ki67P and Embryonic Tissue
 Thyroid Antibodies  >231 publications with Ki67P and Thyroid
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