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KISS1 elisa kit :: Rat KiSS-1 metastasis-suppressor ELISA Kit

Scan QR to view Datasheet Catalog #    MBS704611
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

KiSS-1 metastasis-suppressor (KISS1), ELISA Kit

★Popular Item★
 Also Known As   

Rat Kisspeptin 1, KISS1 ELISA Kit

 Product Synonym Names    Rat Kisspeptin 1 (KISS1) ELISA Kit; KiSS-1; METASTIN; MGC39258; kisspeptin; malignant melanoma metastasis-suppressor; KiSS-1 metastasis-suppressor
 Product Gene Name   

KISS1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS704611 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q7TSB7
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Rat KISS1. No significant cross-reactivity or interference between Rat KISS1 and analogues was observed.
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 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of KISS1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for KISS1 purchase    MBS704611 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the KiSS-1 metastasis-suppressor (KISS1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KISS1. The ELISA analytical biochemical technique of the MBS704611 kit is based on KISS1 antibody-KISS1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KISS1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KISS1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for KISS1 elisa kit

   Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to KISS1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for KISS1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain KISS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of KISS1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of KISS1 elisa kit    KISS1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS704611. Click to request current manual
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NCBI/Uniprot data below describe general gene information for KISS1. It may not necessarily be applicable to this product.
 NCBI GI #    32140194
 NCBI GeneID    289023
 NCBI Accession #    NP_859043.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_181692.1 [Other Products]
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 UniProt Primary Accession #    Q7TSB7 [Other Products]
 UniProt Related Accession #    Q7TSB7 [Other Products]
 Molecular Weight    14,188 Da
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 NCBI Official Full Name    metastasis-suppressor KiSS-1
 NCBI Official Synonym Full Names    KiSS-1 metastasis-suppressor
 NCBI Official Symbol    Kiss1 [Similar Products]
 NCBI Official Synonym Symbols   
Eseptin
[Similar Products]
 NCBI Protein Information    metastasis-suppressor KiSS-1; metastin; kisspeptin-1; MLL septin-like fusion; Kiss1 variant E1a-E2b-E3; Kiss1 variant E1b-E2a-E3; Kiss1 variant E1b-E2b-E3
 UniProt Protein Name    Metastasis-suppressor KiSS-1
 UniProt Synonym Protein Names   
Kisspeptin-1Cleaved into the following 2 chains:Metastin; Kisspeptin-10Alternative name(s):Metastin45-54
 Protein Family    Metastasis-suppressor
 UniProt Gene Name    Kiss1 [Similar Products]
 UniProt Entry Name    KISS1_RAT
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 NCBI Summary for KISS1    ligand for the G-protein-coupled receptor GPR54; may be involved in autonomic and sensory neural signaling [RGD, Feb 2006]
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 UniProt Comments for KISS1    Function: Metastasis suppressor protein. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. Intracerebroventricular administration induces an increase in serum LH and FSH levels in prepubertal male and female as well as in adult animals.3 PublicationsManual assertion based on experiment in:Ref.2
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 Research Articles on KISS1    1. Data suggest age-related changes in hypothalamo-pituitary axis responsiveness via Kiss1/Kiss1 receptor signaling reflect reduced insulin-like growth factor 1 (Igf1)/Igf1 receptor signaling with aging of neuroendocrine cells of hypothalamus.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with KISS1 elisa kitPathways associated with KISS1 elisa kit
 Reference Product  PubMed Publications
 Kiss1r elisa kit  >313 publications with KISS1 and Kiss1r
 Sox13 elisa kit  >1 publications with KISS1 and Sox13
 Etnk2 elisa kit  >1 publications with KISS1 and Etnk2
 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram
Diseases associated with KISS1 elisa kitOrgans/Tissues associated with KISS1 elisa kit
 Disease Name  Pubmed Publications
 Male Urogenital Diseases Antibodies  >50 publications with KISS1 and Male Urogenital Diseases
 Genital Diseases, Female Antibodies  >46 publications with KISS1 and Genital Diseases, Female
 Puberty, Precocious Antibodies  >44 publications with KISS1 and Puberty, Precocious
 Infertility, Female Antibodies  >40 publications with KISS1 and Infertility, Female
 Breast Neoplasms Antibodies  >38 publications with KISS1 and Breast Neoplasms
 Pregnancy Complications Antibodies  >31 publications with KISS1 and Pregnancy Complications
 Puberty, Delayed Antibodies  >31 publications with KISS1 and Puberty, Delayed
 Adenocarcinoma Antibodies  >27 publications with KISS1 and Adenocarcinoma
 Infertility, Male Antibodies  >26 publications with KISS1 and Infertility, Male
 Ovarian Diseases Antibodies  >21 publications with KISS1 and Ovarian Diseases
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with KISS1 and Bone
 Connective Tissue Antibodies  >4 publications with KISS1 and Connective Tissue
 Heart Antibodies  >3 publications with KISS1 and Heart
 Muscle Antibodies  >3 publications with KISS1 and Muscle
 Brain Antibodies  >3 publications with KISS1 and Brain
 Vascular Antibodies  >2 publications with KISS1 and Vascular
 Embryonic Tissue Antibodies  >2 publications with KISS1 and Embryonic Tissue
 Ear Antibodies  >1 publications with KISS1 and Ear
 Kidney Antibodies  >1 publications with KISS1 and Kidney
 Umbilical Cord Antibodies  >1 publications with KISS1 and Umbilical Cord
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