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LAMB2 elisa kit :: Rat Laminin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS824860
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Tests  /  $470 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Laminin (LAMB2), ELISA Kit

★Popular Item★
 Also Known As   

Rat Laminin ELISA Kit

 Product Synonym Names    LAMS; Laminin subunit beta-2; Laminin B1s chain; Laminin-11 subunit beta; Laminin-14 subunit beta; Laminin-15 subunit beta; Laminin-3 subunit beta; Laminin-4 subunit beta; Laminin-7 subunit beta; Laminin-9 subunit beta; S-laminin subunit beta; S-LAM beta
 Product Gene Name   

LAMB2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P15800
 Species Reactivity    Rat
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 Specificity    The Rat Laminin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat Laminin proteins within the range of 156 pg/ml - 10000 pg/ml.
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 Samples    Cell Culture Supernatants, Serum, Plasma
 Sensitivity    10 pg/ml.
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 Preparation and Storage    Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of LAMB2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for LAMB2 purchase    MBS824860 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Laminin (LAMB2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LAMB2, with an estimated sensitivity of 10 pg/ml. The ELISA analytical biochemical technique of the MBS824860 kit is based on LAMB2 antibody-LAMB2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LAMB2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LAMB2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell Culture Supernatants, Serum, Plasma. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for LAMB2 elisa kit

   Principle of the Assay: The Rat Laminin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat Laminin in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Rat Laminin coated on a 96-well plate. Standards and samples are pipetted into the wells and Laminin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat Laminin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Laminin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2. Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation. The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids. The human laminin A chain is at locus 18p11.3. The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide. The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31. The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology. The human laminin B2 chain gene maps to the long arm of chromosome 1 in the band q31.
 Applications Tested/Suitable for LAMB2 elisa kit   

Sandwich ELISA (SE)

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 Typical Testing Data/Standard Curve (for reference only) of LAMB2 elisa kit    LAMB2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS824860. Click to request current manual
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NCBI/Uniprot data below describe general gene information for LAMB2. It may not necessarily be applicable to this product.
 NCBI GI #    6981142
 NCBI GeneID    25473
 NCBI Accession #    NP_037106.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_012974.1 [Other Products]
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 UniProt Primary Accession #    P15800 [Other Products]
 UniProt Related Accession #    P15800 [Other Products]
 Molecular Weight    196,474 Da
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 NCBI Official Full Name    laminin subunit beta-2
 NCBI Official Synonym Full Names    laminin, beta 2
 NCBI Official Symbol    Lamb2 [Similar Products]
 NCBI Official Synonym Symbols   
SLAM
[Similar Products]
 NCBI Protein Information    laminin subunit beta-2; S-LAM beta; laminin chain B3; laminin chain beta 2; S-laminin subunit beta; laminin-3 subunit beta; laminin-4 subunit beta; laminin-7 subunit beta; laminin-9 subunit beta; laminin-11 subunit beta; laminin-14 subunit beta; laminin-15 subunit beta
 UniProt Protein Name    Laminin subunit beta-2
 UniProt Synonym Protein Names   
Laminin chain B3; Laminin-11 subunit beta; Laminin-14 subunit beta; Laminin-15 subunit beta; Laminin-3 subunit beta; Laminin-4 subunit beta; Laminin-7 subunit beta; Laminin-9 subunit beta; S-laminin subunit beta; S-LAM beta
 Protein Family    Laminin
 UniProt Gene Name    Lamb2 [Similar Products]
 UniProt Synonym Gene Names    S-LAM beta [Similar Products]
 UniProt Entry Name    LAMB2_RAT
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 NCBI Summary for LAMB2    a component of the basal lamina; may be involved in motor neuron function [RGD, Feb 2006]
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 UniProt Comments for LAMB2    LAMB2: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Defects in LAMB2 are the cause of Pierson syndrome (PIERSS); also known as microcoria-congenital nephrotic syndrome. Pierson syndrome is characterized by nephrotic syndrome with neonatal onset, diffuse mesangial sclerosis and eye abnormalities with microcoria as the leading clinical feature. Death usually occurs within the first weeks of life. Disease severity depends on the mutation type: nontruncating LAMB2 mutations may display variable phenotypes ranging from a milder variant of Pierson syndrome to isolated congenital nephrotic syndrome. Defects in LAMB2 are the cause of nephrotic syndrome type 5 with or without ocular abnormalities (NPHS5). NPHS5 is a renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure. NPHS5 is characterized by very early onset of progressive renal failure. A subset of patients may develop mild ocular anomalies, such as myopia, nystagmus, and strabismus.

Protein type: Secreted; Extracellular matrix; Motility/polarity/chemotaxis; Secreted, signal peptide

Cellular Component: extracellular matrix; laminin-3 complex; basement membrane; basal lamina; synapse

Molecular Function: integrin binding

Biological Process: cellular morphogenesis during differentiation; axon guidance; Schwann cell development; visual perception; retina development in camera-type eye; axon extension involved in regeneration; synapse organization and biogenesis; cell adhesion; astrocyte development; neurite development; neuromuscular junction development
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 Research Articles on LAMB2    1. Together, these studies provide evidence for regulation of Lamb2 translation by IMP2, an RNA binding protein that targets Lamb2 mRNA to the actin cytoskeleton.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with LAMB2 elisa kitDiseases associated with LAMB2 elisa kit
 Products by Pathway  Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 ECM Proteoglycans Pathway antibodies  ECM Proteoglycans Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Inflammatory Response Pathway antibodies  Inflammatory Response Pathway Diagram
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >39 publications with LAMB2 and Kidney Diseases
 Congenital Abnormalities Antibodies  >26 publications with LAMB2 and Congenital Abnormalities
 Pierson syndrome Antibodies  >26 publications with LAMB2 and Pierson syndrome
 Proteinuria Antibodies  >14 publications with LAMB2 and Proteinuria
 Disease Models, Animal Antibodies  >4 publications with LAMB2 and Disease Models, Animal
 Cataract Antibodies  >3 publications with LAMB2 and Cataract
 Renal Insufficiency Antibodies  >2 publications with LAMB2 and Renal Insufficiency
 Abnormalities, Drug-Induced Antibodies  >1 publications with LAMB2 and Abnormalities, Drug-Induced
 Heart Diseases Antibodies  >1 publications with LAMB2 and Heart Diseases
 Weight Gain Antibodies  >1 publications with LAMB2 and Weight Gain
Organs/Tissues associated with LAMB2 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >27 publications with LAMB2 and Kidney
 Eye Antibodies  >23 publications with LAMB2 and Eye
 Brain Antibodies  >4 publications with LAMB2 and Brain
 Liver Antibodies  >2 publications with LAMB2 and Liver
 Ovary Antibodies  >2 publications with LAMB2 and Ovary
 Artery Antibodies  >1 publications with LAMB2 and Artery
 Embryonic Tissue Antibodies  >1 publications with LAMB2 and Embryonic Tissue
 Prostate Antibodies  >1 publications with LAMB2 and Prostate
 Lung Antibodies  >1 publications with LAMB2 and Lung
 Colon Antibodies  >1 publications with LAMB2 and Colon
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