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MBP elisa kit :: Rat MBP ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2508600
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

MBP, ELISA Kit

★Popular Item★
 Also Known As   

Rat MBP (Myelin Basic Protein) ELISA Kit

 Product Gene Name   

MBP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2508600 COA    COA PDF
 OMIM    159430
 3D Structure    ModBase 3D Structure for P02686
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 Species Reactivity    Rat
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 Specificity    This kit recognizes natural and recombinant Rat MBP. No significant cross-reactivity or interference between Rat MBP and analogues was observed.
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    The minimum detectable dose of Rat MBP is 0.094ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MBP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MBP purchase    MBS2508600 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the MBP, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing MBP, with an estimated sensitivity of The minimum detectable dose of Rat MBP is 0.094ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero). The ELISA analytical biochemical technique of the MBS2508600 kit is based on MBP antibody-MBP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MBP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MBP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for MBP elisa kit

   Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MBP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MBP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MBP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of MBP. You can calculate the concentration of MBP in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of MBP elisa kit    MBP elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2508600. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MBP. It may not necessarily be applicable to this product.
 NCBI GI #    49456793
 NCBI GeneID    4155
 NCBI Accession #    CAG46717.1 [Other Products]
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 UniProt Primary Accession #    P02686 [Other Products]
 UniProt Secondary Accession #    Q15337; Q15338; Q15339; Q15340; A4FU54; A6NI84; A8MY86; A8MYL4; B3KY66; B7ZKS2; B7ZKS4 [Other Products]
 UniProt Related Accession #    P02686 [Other Products]
 Molecular Weight    17,343 Da
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 NCBI Official Full Name    MBP, partial
 NCBI Official Synonym Full Names    myelin basic protein
 NCBI Official Symbol    MBP [Similar Products]
 NCBI Protein Information    Golli-MBP; myelin basic protein; myelin A1 protein; myelin membrane encephalitogenic protein
 UniProt Protein Name    Myelin basic protein
 UniProt Synonym Protein Names   
Myelin A1 protein; Myelin membrane encephalitogenic protein
 Protein Family    Myelin basic protein
 UniProt Gene Name    MBP [Similar Products]
 UniProt Synonym Gene Names    MBP [Similar Products]
 UniProt Entry Name    MBP_HUMAN
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 NCBI Summary for MBP    The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq, Jul 2008]
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 UniProt Comments for MBP    MBP: The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non- classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T- cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. Homodimer. Isoform 3 exists as a homodimer. MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. Belongs to the myelin basic protein family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 18q23

Cellular Component: compact myelin; internode region of axon; cell soma; plasma membrane; nucleus

Molecular Function: protein binding; structural constituent of myelin sheath; protease binding

Biological Process: response to mercury ion; myelination; synaptic transmission; substantia nigra development; central nervous system development; response to toxin; negative regulation of axonogenesis; immune response; axon ensheathment; response to progesterone stimulus; aging
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 Research Articles on MBP    1. data reveal the first case of a pathophysiologically important autoantigen as a ubiquitin-independent substrate of the 26S proteasome.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with MBP elisa kitDiseases associated with MBP elisa kit
 Products by Pathway  Pathway Diagram
 Glial Cell Differentiation Pathway antibodies  Glial Cell Differentiation Pathway Diagram
 MAPK Cascade Pathway antibodies  MAPK Cascade Pathway Diagram
 Neural Crest Differentiation Pathway antibodies  Neural Crest Differentiation Pathway Diagram
 Spinal Cord Injury Pathway antibodies  Spinal Cord Injury Pathway Diagram
 Disease Name  Pubmed Publications
 Demyelinating Diseases Antibodies  >1970 publications with MBP and Demyelinating Diseases
 Brain Diseases Antibodies  >1723 publications with MBP and Brain Diseases
 Disease Models, Animal Antibodies  >1385 publications with MBP and Disease Models, Animal
 Encephalomyelitis, Autoimmune, Experimental Antibodies  >1189 publications with MBP and Encephalomyelitis, Autoimmune, Experimental
 Cardiovascular Diseases Antibodies  >687 publications with MBP and Cardiovascular Diseases
 Inflammation Antibodies  >487 publications with MBP and Inflammation
 Hypertension Antibodies  >411 publications with MBP and Hypertension
 Necrosis Antibodies  >259 publications with MBP and Necrosis
 Heart Diseases Antibodies  >190 publications with MBP and Heart Diseases
 Kidney Diseases Antibodies  >152 publications with MBP and Kidney Diseases
Organs/Tissues associated with MBP elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >3621 publications with MBP and Blood
 Brain Antibodies  >1906 publications with MBP and Brain
 Nerve Antibodies  >1413 publications with MBP and Nerve
 Heart Antibodies  >684 publications with MBP and Heart
 Spinal Cord Antibodies  >678 publications with MBP and Spinal Cord
 Kidney Antibodies  >267 publications with MBP and Kidney
 Muscle Antibodies  >246 publications with MBP and Muscle
 Spleen Antibodies  >240 publications with MBP and Spleen
 Lymph Node Antibodies  >239 publications with MBP and Lymph Node
 Lymph Antibodies  >239 publications with MBP and Lymph
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