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CCL2 elisa kit :: Rat MCP1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS824584
Typical Standard Curve/Testing Data
Unit / Price
96 Tests  /  $470 +1 FREE 8GB USB
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 Product Name   


★Popular Item★
 Also Known As   


 Product Synonym Names    MCP1; SCYA2; C-C motif chemokine 2; HC11; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; MCAF; Monocyte chemotactic protein 1; MCP-1; Monocyte secretory protein JE; Small-inducible cytokine A2
 Product Gene Name   

CCL2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P14844
 Species Reactivity    Rat
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 Specificity    The Rat MCP1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat MCP1 proteins within the range of 15.6 pg/ml - 1000 pg/ml.
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 Samples    Cell Culture Supernatants, Serum
 Sensitivity    Typically less than 10 pg/ml.
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 Preparation and Storage    Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CCL2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CCL2 purchase    MBS824584 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the MCP1 (CCL2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CCL2, with an estimated sensitivity of Typically less than 10 pg/ml. The ELISA analytical biochemical technique of the MBS824584 kit is based on CCL2 antibody-CCL2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CCL2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CCL2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell Culture Supernatants, Serum. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CCL2 elisa kit

   Principle of the Assay: The Rat MCP1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat MCP1 in Cell Culture Supernatants, Serum. This assay employs an antibody specific for Rat MCP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat MCP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids. MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder.
 Applications Tested/Suitable for CCL2 elisa kit   

Sandwich ELISA (SE)

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 Typical Standard Curve/Testing Data of CCL2 elisa kit    CCL2 elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS824584. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CCL2. It may not necessarily be applicable to this product.
 NCBI GI #    13928714
 NCBI GeneID    24770
 NCBI Accession #    NP_113718.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_031530.1 [Other Products]
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 UniProt Primary Accession #    P14844 [Other Products]
 UniProt Secondary Accession #    Q549R5 [Other Products]
 UniProt Related Accession #    P14844 [Other Products]
 Molecular Weight    16,460 Da
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 NCBI Official Full Name    C-C motif chemokine 2
 NCBI Official Synonym Full Names    chemokine (C-C motif) ligand 2
 NCBI Official Symbol    Ccl2 [Similar Products]
 NCBI Official Synonym Symbols   
MCP-1; Scya2; Sigje
[Similar Products]
 NCBI Protein Information    C-C motif chemokine 2; small inducible gene JE; small inducible cytokine A2; small-inducible cytokine A2; monocyte chemotactic protein 1; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; immediate-early serum-responsive JE protein; immediate-early serum-responsive protein JE
 UniProt Protein Name    C-C motif chemokine 2
 UniProt Synonym Protein Names   
Immediate-early serum-responsive protein JE; Monocyte chemoattractant protein 1; Monocyte chemotactic protein 1; MCP-1; Small-inducible cytokine A2
 Protein Family    C-C motif chemokine
 UniProt Gene Name    Ccl2 [Similar Products]
 UniProt Synonym Gene Names    Je; Mcp1; Scya2; MCP-1 [Similar Products]
 UniProt Entry Name    CCL2_RAT
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 NCBI Summary for CCL2    a monocyte chemoattractant protein [RGD, Feb 2006]
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 UniProt Comments for CCL2    CCL13: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens. By IL1/interleukin-1 and TNF. Widely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis; Chemokine

Cellular Component: extracellular space; cell soma; rough endoplasmic reticulum; endocytic vesicle; perinuclear region of cytoplasm; dendrite; cytoplasm; extracellular region; synapse; perikaryon; nerve terminal

Molecular Function: heparin binding; G-protein-coupled receptor binding; CCR2 chemokine receptor binding; chemokine activity

Biological Process: maternal process involved in pregnancy; positive regulation of cell adhesion; positive regulation of leukocyte migration; response to glucocorticoid stimulus; response to lipopolysaccharide; response to antibiotic; monocyte chemotaxis; regulation of cell shape; transforming growth factor beta receptor signaling pathway; response to vitamin B3; negative regulation of neuron apoptosis; response to drug; neutrophil chemotaxis; organ regeneration; positive regulation of tumor necrosis factor production; response to amino acid stimulus; response to ethanol; response to bacterium; cellular response to insulin stimulus; response to heat; response to mechanical stimulus; positive regulation of endothelial cell proliferation; response to activity; response to progesterone stimulus; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of collagen biosynthetic process; chemokinesis; positive regulation of synaptic transmission; positive regulation of cellular extravasation; response to wounding; positive regulation of cell-cell adhesion; lipopolysaccharide-mediated signaling pathway; protein kinase B signaling cascade; response to gamma radiation; inflammatory response; lymphocyte chemotaxis; aging; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; cytoskeleton organization and biogenesis; glial cell migration; macrophage chemotaxis; leukocyte migration during inflammatory response; cellular calcium ion homeostasis; negative regulation of angiogenesis; positive regulation of leukocyte mediated cytotoxicity; maternal process involved in parturition; response to hypoxia; positive regulation of T cell activation; vascular endothelial growth factor receptor signaling pathway; astrocyte cell migration
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 Research Articles on CCL2    1. This study indicates that TNF-alpha was released from the activated microglia induced by retinal neuronal MCP-1 via the p38, ERK, and NF-kappaB pathways, but not c-Jun N-terminal kinase.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CCL2 elisa kitPathways associated with CCL2 elisa kit
 Reference Product  PubMed Publications
 Tnf elisa kit  >1938 publications with CCL2 and Tnf
 Ccr2 elisa kit  >1229 publications with CCL2 and Ccr2
 Ccl3 elisa kit  >893 publications with CCL2 and Ccl3
 Icam1 elisa kit  >675 publications with CCL2 and Icam1
 Ccr5 elisa kit  >329 publications with CCL2 and Ccr5
 Il6 elisa kit  >265 publications with CCL2 and Il6
 Ccl7 elisa kit  >253 publications with CCL2 and Ccl7
 Ccr1 elisa kit  >178 publications with CCL2 and Ccr1
 Cx3cr1 elisa kit  >118 publications with CCL2 and Cx3cr1
 Il1b elisa kit  >53 publications with CCL2 and Il1b
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 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
Diseases associated with CCL2 elisa kitOrgans/Tissues associated with CCL2 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >4331 publications with CCL2 and Inflammation
 Necrosis Antibodies  >2525 publications with CCL2 and Necrosis
 Cardiovascular Diseases Antibodies  >1835 publications with CCL2 and Cardiovascular Diseases
 Atherosclerosis Antibodies  >1032 publications with CCL2 and Atherosclerosis
 Myocardial Ischemia Antibodies  >396 publications with CCL2 and Myocardial Ischemia
 Liver Diseases Antibodies  >345 publications with CCL2 and Liver Diseases
 Hypersensitivity Antibodies  >322 publications with CCL2 and Hypersensitivity
 Glomerulonephritis Antibodies  >286 publications with CCL2 and Glomerulonephritis
 Lung Injury Antibodies  >245 publications with CCL2 and Lung Injury
 Asthma Antibodies  >210 publications with CCL2 and Asthma
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >1076 publications with CCL2 and Lung
 Liver Antibodies  >658 publications with CCL2 and Liver
 Heart Antibodies  >650 publications with CCL2 and Heart
 Connective Tissue Antibodies  >425 publications with CCL2 and Connective Tissue
 Prostate Antibodies  >96 publications with CCL2 and Prostate
 Dorsal Root Ganglion Antibodies  >55 publications with CCL2 and Dorsal Root Ganglion
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