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MT elisa kit :: Rat Metallothionein, MT ELISA Kit

Scan QR to view Datasheet Catalog #    MBS724498
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
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 Product Name   

Metallothionein, MT, ELISA Kit

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 Also Known As   

Rat Metallothionein, MT ELISA Kit

 Product Synonym Names    Rat Metallothionein, MT ELISA Kit; Metallothionein, MT; Metallothionein, MT ELISA Kit (Rat)
 Product Gene Name   

MT elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 11; NC_006098.2 (2100369..2100601, complement)
 3D Structure    ModBase 3D Structure for P68497
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of MET. No significant cross-reactivity or interference between MET and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MET and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    0.1 ng/mL.
 Intended Uses    This MET ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat MET. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MT purchase    MBS724498 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Metallothionein, MT, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MT, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS724498 kit is based on MT antibody-MT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for MT elisa kit

   Principle of the Assay: MET ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MET antibody and an MET-HRP conjugate. The assay sample and buffer are incubated together with MET-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MET concentration since MET from samples and MET-HRP conjugate compete for the anti-MET antibody binding site. Since the number of sites is limited, as more sites are occupied by MET from the sample, fewer sites are left to bind MET-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MET concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for MT elisa kit    Rat ELISA Kit
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 Typical Testing Data/Standard Curve (for reference only) of MT elisa kit    MT elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS724498. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MT. It may not necessarily be applicable to this product.
 NCBI GI #    46048711
 NCBI GeneID    396212
 NCBI Accession #    NP_990606.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_205275.1 [Other Products]
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 UniProt Primary Accession #    P68497 [Other Products]
 UniProt Secondary Accession #    P09576 [Other Products]
 UniProt Related Accession #    P68497 [Other Products]
 Molecular Weight    6,462 Da
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 NCBI Official Full Name    metallothionein
 NCBI Official Synonym Full Names    metallothionein 4<
 NCBI Official Symbol    MT4 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    metallothionein; MT; metallothionein 2A
 UniProt Protein Name    Metallothionein
 UniProt Entry Name    MT_CHICK
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 UniProt Comments for MT    Function: Metallothioneins have a high content of cysteine residues that bind various heavy metals.

Domain: Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.

Sequence similarities: Belongs to the metallothionein superfamily. Type 1 family.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with MT elisa kitOrgans/Tissues associated with MT elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >35 publications with MT and Neoplasms
 Drug Toxicity Antibodies  >12 publications with MT and Drug Toxicity
 Cell Transformation, Neoplastic Antibodies  >11 publications with MT and Cell Transformation, Neoplastic
 Carcinoma Antibodies  >10 publications with MT and Carcinoma
 Inflammation Antibodies  >10 publications with MT and Inflammation
 Breast Neoplasms Antibodies  >7 publications with MT and Breast Neoplasms
 Central Nervous System Diseases Antibodies  >3 publications with MT and Central Nervous System Diseases
 Lung Neoplasms Antibodies  >2 publications with MT and Lung Neoplasms
 Skin Neoplasms Antibodies  >2 publications with MT and Skin Neoplasms
 Anemia Antibodies  >1 publications with MT and Anemia
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >133 publications with MT and Blood
 Brain Antibodies  >11 publications with MT and Brain
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