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Smad2 elisa kit :: Rat Mothers Against Decapentaplegic Homolog 2 (Smad2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2024882 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $285 +1 FREE 8GB USB
48-Strip-Wells  /  $530 +1 FREE 8GB USB
96-Strip-Wells  /  $715 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,910 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,280 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Mothers Against Decapentaplegic Homolog 2 (Smad2), ELISA Kit

★Popular Item★
 Also Known As   

Mothers Against Decapentaplegic Homolog 2 (Smad2) ELISA Kit

 Product Synonym Names    JV18; JV18-1; MADH2; MADR2; HMAD-2; HSMAD2; Mad-related protein 2
 Product Gene Name   

Smad2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    601366
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mothers Against Decapentaplegic Homolog 2 (Smad2).
No significant cross-reactivity or interference between Mothers Against Decapentaplegic Homolog 2 (Smad2) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates, Cell lysates and Other Biological Fluids
 Detection Range    0.156-10ng/mL
 Sensitivity    < 0.058ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Mothers Against Decapentaplegic Homolog 2 (Smad2) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Mothers Against Decapentaplegic Homolog 2 (Smad2) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Smad2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Smad2 purchase    MBS2024882 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mothers Against Decapentaplegic Homolog 2 (Smad2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing Smad2, with an estimated sensitivity of < 0.058ng/mL. The ELISA analytical biochemical technique of the MBS2024882 kit is based on Smad2 antibody-Smad2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Smad2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Smad2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Smad2 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mothers Against Decapentaplegic Homolog 2 (Smad2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mothers Against Decapentaplegic Homolog 2 (Smad2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mothers Against Decapentaplegic Homolog 2 (Smad2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mothers Against Decapentaplegic Homolog 2 (Smad2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for Smad2 elisa kit    Signal transduction; Apoptosis; Tumor immunity
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 Typical Testing Data/Standard Curve (for reference only) of Smad2 elisa kit    Smad2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2024882. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Smad2. It may not necessarily be applicable to this product.
 NCBI GI #    209693426
 NCBI GeneID    4087
 NCBI Accession #    NP_001129409.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001135937.2 [Other Products]
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 UniProt Related Accession #    Q15796 [Other Products]
 Molecular Weight    48,956 Da
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 NCBI Official Full Name    mothers against decapentaplegic homolog 2 isoform 2
 NCBI Official Synonym Full Names    SMAD family member 2
 NCBI Official Symbol    SMAD2 [Similar Products]
 NCBI Official Synonym Symbols   
JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
[Similar Products]
 NCBI Protein Information    mothers against decapentaplegic homolog 2
 UniProt Protein Name    Mothers against decapentaplegic homolog 2
 UniProt Synonym Protein Names   
JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2
 Protein Family    Mothers against decapentaplegic
 UniProt Gene Name    SMAD2 [Similar Products]
 UniProt Synonym Gene Names    MADH2; MADR2; MAD homolog 2; Mothers against DPP homolog 2; hMAD-2; SMAD 2; Smad2; hSMAD2 [Similar Products]
 UniProt Entry Name    SMAD2_HUMAN
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 NCBI Summary for Smad2    The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]
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 UniProt Comments for Smad2    SMAD2: ubiquitously expressed transcription factor phosphorylated and activated by TGF-beta receptor-type kinases. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis. Phosphorylated Smads dimerize with collaborating Smad4 and are translocated into the nucleus, where the transcription of target genes is stimulated. Two alternatively spliced isoforms have been described.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin; cytoplasm; nucleus; cytosol

Molecular Function: transcription activator binding; metal ion binding; transcription factor binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; phosphatase binding; protein binding; DNA binding; ubiquitin protein ligase binding; double-stranded DNA binding; chromatin binding; SMAD binding; transforming growth factor beta receptor binding; transcription factor activity

Biological Process: developmental growth; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; paraxial mesoderm morphogenesis; gastrulation; primary microRNA processing; palate development; negative regulation of transcription from RNA polymerase II promoter; post-embryonic development; anterior/posterior pattern formation; negative regulation of cell proliferation; regulation of transforming growth factor beta receptor signaling pathway; ureteric bud development; transforming growth factor beta receptor signaling pathway; pancreas development; embryonic foregut morphogenesis; response to glucose stimulus; positive regulation of BMP signaling pathway; transcription initiation from RNA polymerase II promoter; pericardium development; cell fate commitment; regulation of binding; transcription, DNA-dependent; in utero embryonic development; common-partner SMAD protein phosphorylation; embryonic cranial skeleton morphogenesis; SMAD protein complex assembly; organ growth; zygotic determination of dorsal/ventral axis; mesoderm formation; insulin secretion; endoderm formation; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of transforming growth factor beta receptor signaling pathway; lung development
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 Research Articles on Smad2    1. A role for decorin and p-Smad-2 in the pathophysiology of fetal membranes and adverse pregnancy outcomes.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Smad2 elisa kitPathways associated with Smad2 elisa kit
 Reference Product  PubMed Publications
 SMAD4 elisa kit  >883 publications with Smad2 and SMAD4
 SKI elisa kit  >63 publications with Smad2 and SKI
 FOXH1 elisa kit  >59 publications with Smad2 and FOXH1
 SMURF2 elisa kit  >52 publications with Smad2 and SMURF2
 SP1 elisa kit  >41 publications with Smad2 and SP1
 SKIL elisa kit  >39 publications with Smad2 and SKIL
 TGFBR1 elisa kit  >30 publications with Smad2 and TGFBR1
 CREBBP elisa kit  >7 publications with Smad2 and CREBBP
 UBC elisa kit  >4 publications with Smad2 and UBC
 ZFYVE9 elisa kit  >1 publications with Smad2 and ZFYVE9
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Activin Signaling Pathway antibodies  Activin Signaling Pathway Diagram
 Activin Signaling Pathway antibodies  Activin Signaling Pathway Diagram
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 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
Diseases associated with Smad2 elisa kitOrgans/Tissues associated with Smad2 elisa kit
 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >855 publications with Smad2 and Fibrosis
 Carcinoma Antibodies  >370 publications with Smad2 and Carcinoma
 Disease Models, Animal Antibodies  >350 publications with Smad2 and Disease Models, Animal
 Inflammation Antibodies  >271 publications with Smad2 and Inflammation
 Kidney Diseases Antibodies  >218 publications with Smad2 and Kidney Diseases
 Liver Diseases Antibodies  >188 publications with Smad2 and Liver Diseases
 Adenocarcinoma Antibodies  >161 publications with Smad2 and Adenocarcinoma
 Heart Diseases Antibodies  >148 publications with Smad2 and Heart Diseases
 Breast Neoplasms Antibodies  >124 publications with Smad2 and Breast Neoplasms
 Hypertrophy Antibodies  >107 publications with Smad2 and Hypertrophy
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >668 publications with Smad2 and Muscle
 Bone Antibodies  >485 publications with Smad2 and Bone
 Kidney Antibodies  >404 publications with Smad2 and Kidney
 Blood Antibodies  >396 publications with Smad2 and Blood
 Lung Antibodies  >389 publications with Smad2 and Lung
 Vascular Antibodies  >380 publications with Smad2 and Vascular
 Liver Antibodies  >301 publications with Smad2 and Liver
 Connective Tissue Antibodies  >272 publications with Smad2 and Connective Tissue
 Heart Antibodies  >264 publications with Smad2 and Heart
 Embryonic Tissue Antibodies  >241 publications with Smad2 and Embryonic Tissue
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