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MPO elisa kit :: Rat Myeloperoxidase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS724567
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Myeloperoxidase (MPO), ELISA Kit

★Popular Item★
 Also Known As   

Rat Myeloperoxidase ELISA Kit

 Product Gene Name   

MPO elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    606989
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of MPO. No significant cross-reactivity or interference between MPO and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MPO and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    2.5-50ng/mL
 Sensitivity    0.1ng/ml
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MPO elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MPO purchase    MBS724567 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Myeloperoxidase (MPO) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 2.5-50ng/mL in biological research samples containing MPO, with an estimated sensitivity of 0.1ng/ml. The ELISA analytical biochemical technique of the MBS724567 kit is based on MPO antibody-MPO antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MPO antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MPO. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for MPO elisa kit

   Intended Uses: This MPO ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat MPO. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||MPO ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MPO antibody and an MPO-HRP conjugate. The assay sample and buffer are incubated together with MPO-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MPO concentration since MPO from samples and MPO-HRP conjugate compete for the anti-MPO antibody binding site. Since the number of sites is limited, as more sites are occupied by MPO from the sample, fewer sites are left to bind MPO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MPO concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for MPO elisa kit    Immunology
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 Typical Testing Data/Standard Curve of MPO elisa kit    MPO elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS724567. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MPO. It may not necessarily be applicable to this product.
 NCBI GI #    188658
 NCBI GeneID    4353
 NCBI Accession #    AAA59863.1 [Other Products]
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 UniProt Secondary Accession #    Q14862; Q4PJH5; Q9UCL7; A1L4B8 [Other Products]
 UniProt Related Accession #    P05164 [Other Products]
 Molecular Weight    87,248 Da
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 NCBI Official Full Name    myeloperoxidase
 NCBI Official Synonym Full Names    myeloperoxidase
 NCBI Official Symbol    MPO [Similar Products]
 NCBI Protein Information    myeloperoxidase
 UniProt Protein Name    Myeloperoxidase
 Protein Family    Myeloperoxidase
 UniProt Gene Name    MPO [Similar Products]
 UniProt Synonym Gene Names    MPO [Similar Products]
 UniProt Entry Name    PERM_HUMAN
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 NCBI Summary for MPO    Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils. [provided by RefSeq, Jul 2008]
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 UniProt Comments for MPO    MPO: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain. Belongs to the peroxidase family. XPO subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.11.2.2

Chromosomal Location of Human Ortholog: 17q23.1

Cellular Component: extracellular space; azurophil granule; mitochondrion; lysosome; nucleus; secretory granule

Molecular Function: heparin binding; peroxidase activity; metal ion binding; heme binding; chromatin binding

Biological Process: response to food; respiratory burst during defense response; removal of superoxide radicals; hydrogen peroxide catabolic process; response to mechanical stimulus; defense response; response to lipopolysaccharide; response to yeast; response to oxidative stress; defense response to fungus; negative regulation of apoptosis; aging

Disease: Myeloperoxidase Deficiency; Alzheimer Disease
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 Research Articles on MPO    1. Genetic variations in radiation and chemotherapy drug action pathways and survival in locoregionally advanced nasopharyngeal carcinoma treated with chemoradiotherapy.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MPO elisa kitPathways associated with MPO elisa kit
 Reference Product  PubMed Publications
 PRTN3 elisa kit  >200 publications with MPO and PRTN3
 MYB elisa kit  >11 publications with MPO and MYB
 JAK2 elisa kit  >5 publications with MPO and JAK2
 ALB elisa kit  >4 publications with MPO and ALB
 ELANE elisa kit  >2 publications with MPO and ELANE
 Products by Pathway  Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Folate Metabolism Pathway antibodies  Folate Metabolism Pathway Diagram
 IL23-mediated Signaling Events Pathway antibodies  IL23-mediated Signaling Events Pathway Diagram
 Phagosome Pathway antibodies  Phagosome Pathway Diagram
 Phagosome Pathway antibodies  Phagosome Pathway Diagram
 Selenium Pathway antibodies  Selenium Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Vitamin B12 Metabolism Pathway antibodies  Vitamin B12 Metabolism Pathway Diagram
 Amb2 Integrin Signaling Pathway antibodies  Amb2 Integrin Signaling Pathway Diagram
Diseases associated with MPO elisa kitOrgans/Tissues associated with MPO elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >2483 publications with MPO and Inflammation
 Cardiovascular Diseases Antibodies  >1979 publications with MPO and Cardiovascular Diseases
 Necrosis Antibodies  >1418 publications with MPO and Necrosis
 Lung Diseases Antibodies  >1147 publications with MPO and Lung Diseases
 Reperfusion Injury Antibodies  >817 publications with MPO and Reperfusion Injury
 Colitis Antibodies  >649 publications with MPO and Colitis
 Kidney Diseases Antibodies  >620 publications with MPO and Kidney Diseases
 Edema Antibodies  >585 publications with MPO and Edema
 Heart Diseases Antibodies  >425 publications with MPO and Heart Diseases
 Hemorrhage Antibodies  >418 publications with MPO and Hemorrhage
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >4824 publications with MPO and Blood
 Lung Antibodies  >1432 publications with MPO and Lung
 Kidney Antibodies  >699 publications with MPO and Kidney
 Brain Antibodies  >570 publications with MPO and Brain
 Bone Antibodies  >381 publications with MPO and Bone
 Bone Marrow Antibodies  >303 publications with MPO and Bone Marrow
 Eye Antibodies  >90 publications with MPO and Eye
 Lymph Node Antibodies  >87 publications with MPO and Lymph Node
 Thyroid Antibodies  >77 publications with MPO and Thyroid
 Embryonic Tissue Antibodies  >41 publications with MPO and Embryonic Tissue
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