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MYH6 elisa kit :: Rat Myosin Heavy Chain 6 Cardiac Muscle Alpha ELISA Kit

Scan QR to view Datasheet Catalog #    MBS753946
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Myosin Heavy Chain 6 Cardiac Muscle Alpha (MYH6), ELISA Kit

 Also Known As   

Rat Myosin Heavy Chain 6 Cardiac Muscle Alpha ELISA Kit

 Product Gene Name   

MYH6 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of CMalphaHc6. No significant cross-reactivity or interference between CMalphaHc6 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CMalphaHc6 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Competitive (Quantitative)
 Sensitivity    0.1 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact technical support.
 Other Notes    Small volumes of MYH6 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MYH6 purchase    MBS753946 is a ready-to-use microwell, strip plate Competitive (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Myosin Heavy Chain 6 Cardiac Muscle Alpha (MYH6) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MYH6, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS753946 kit is based on MYH6 antibody-MYH6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MYH6 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MYH6. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for MYH6 elisa kit

   Intended Uses: This CMalphaHc6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CMalphaHc6. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: CMalphaHc6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CMalphaHc6 antibody and an CMalphaHc6-HRP conjugate. The assay sample and buffer are incubated together with CMalphaHc6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CMalphaHc6 concentration since CMalphaHc6 from samples and CMalphaHc6-HRP conjugate compete for the anti-CMalphaHc6 antibody binding site. Since the number of sites is limited, as more sites are occupied by CMalphaHc6 from the sample, fewer sites are left to bind CMalphaHc6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CMalphaHc6 concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of MYH6 elisa kit    MYH6 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS753946
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NCBI/Uniprot data below describe general gene information for MYH6. It may not necessarily be applicable to this product.
 NCBI GI #    254272872
 NCBI Accession #    BAH85650.1 [Other Products]
 Molecular Weight    24,062 Da
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 NCBI Official Full Name    myosin heavy chain 6 cardiac muscle alpha, partial
 UniProt Protein Name    Myosin heavy chain 6 cardiac muscle alpha
 UniProt Synonym Protein Names   
Myosin heavy chain 6 cardiac muscle alpha
 Protein Family    Myosin
 UniProt Gene Name    Myh6 [Similar Products]
 UniProt Entry Name    C6L7G8_9GOBI
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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