Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow NAT8L rightarrow Rat NAT8L  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • RNASE2 Antibody
 • phosphatase and actin regulator 1 (PHACTR1) ELISA Kit
 • NGF-Receptor (p75) -CD271 (NGFR) Antibody
 • Glial Fibrillary Acidic Protein (GFAP) ELISA Kit
 • 2-oxoglutarate dehydrogenase (OGDH) Antibody
 • IkBa (pS32-36) (NFKB1A) (IkBa (pS32-36) (NFKB1A)) Antibody
 • anti-Protein A (faeC) Antibody
 • E1B protein, large T-antigen (E1B-55K) Recombinant Protein
 • Leukotriene A-4 hydrolase (Lta4h) Recombinant Protein
 • Horse IgE ELISA Kit
 • 8-Hydroxydeoxyguanosine (8-OHdG) ELISA Kit
 • PA-I galactophilic lectin (lecA) Recombinant Protein
 • Interleukin 21 (IL-21) ELISA Kit
 • Triptorelin (Trp) Native Protein
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

NAT8L elisa kit :: Rat N-acetyltransferase 8-like (GCN5-related, putative) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS918714
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

N-acetyltransferase 8-like (GCN5-related, putative) (NAT8L), ELISA Kit

 Also Known As   

Rat N-acetylaspartate synthetase, NAT8L ELISA Kit

 Product Synonym Names    Rat N-acetylaspartate synthetase (NAT8L) ELISA kit; CML3; FLJ37478; MGC117272; NAT8-LIKE; ; N-acetyltransferase 8-like (GCN5-related; putative)
 Product Gene Name   

NAT8L elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS918714 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for D3ZVU9
 Species Reactivity    Rat
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of rat NAT8L. No significant cross-reactivity or interference between rat NAT8L and analogues was observed.
Section Bar
 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    31.25 pg/ml -2000 pg/ml.
 Sensitivity    The minimum detectable dose of rat NAT8L is typically less than 7.81 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Section Bar
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NAT8L elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NAT8L purchase    MBS918714 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the N-acetyltransferase 8-like (GCN5-related, putative) (NAT8L) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25 pg/ml -2000 pg/ml in biological research samples containing NAT8L, with an estimated sensitivity of The minimum detectable dose of rat NAT8L is typically less than 7.81 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS918714 kit is based on NAT8L antibody-NAT8L antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NAT8L antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NAT8L. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Product Description specifically for NAT8L elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NAT8L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NAT8L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAT8L is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAT8L bound in the initial step. The color development is stopped and the intensity of the color is measured.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of NAT8L elisa kit    NAT8L elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS918714. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for NAT8L. It may not necessarily be applicable to this product.
 NCBI GI #    300794296
 NCBI GeneID    289727
 NCBI Accession #    NP_001178610.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001191681.1 [Other Products]
Section Bar
 UniProt Primary Accession #    D3ZVU9 [Other Products]
 UniProt Related Accession #    D3ZVU9 [Other Products]
 Molecular Weight    32,719 Da
Table BarTOPTable Bar
 NCBI Official Full Name    N-acetylaspartate synthetase
 NCBI Official Synonym Full Names    N-acetyltransferase 8-like
 NCBI Official Symbol    Nat8l [Similar Products]
 NCBI Official Synonym Symbols   
RGD1305719
[Similar Products]
 NCBI Protein Information    N-acetylaspartate synthetase; NAA synthetase; N-acetyltransferase 8-like protein
 UniProt Protein Name    N-acetylaspartate synthetase
 UniProt Synonym Protein Names   
N-acetyltransferase 8-like protein (EC:2.3.1.17)
 Protein Family    N-acetylaspartate synthetase
 UniProt Gene Name    Nat8l [Similar Products]
 UniProt Synonym Gene Names    NAA synthetase [Similar Products]
 UniProt Entry Name    NAT8L_RAT
Section Bar
 UniProt Comments for NAT8L    Function: Plays a role in the regulation of lipogenesis by producing N-acetylaspartate acid (NAA), a brain-specific metabolite. NAA occurs in high concentration in brain and its hydrolysis plays a significant part in the maintenance of intact white matter. Promotes dopamine uptake by regulating TNF-alpha expression. Attenuates methamphetamine-induced inhibition of dopamine uptake

Catalytic activity: Acetyl-CoA + L-aspartate = CoA + N-acetyl-L-aspartate.

Enzyme regulation: Aminooxyacetic acid (AOAA) blocks its activity in both cytoplasm and mitochondria
Table BarTOPTable Bar
 Research Articles on NAT8L    1. Asp-NAT/NAT8L and NAA immunoreactivities correlate very well in rat brain, with primary localization in neurons, including cortical pyramidal neurons and granule cells of the hippocampus.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Organs/Tissues associated with NAT8L elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >4 publications with NAT8L and Brain
 Kidney Antibodies  >3 publications with NAT8L and Kidney
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions