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NF-kappaB p65 elisa kit :: Rat NF-kappaB p65 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2505513
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

NF-kappaB p65, ELISA Kit

★Popular Item★
 Also Known As   

Rat NF-kappaB p65(Nuclear Factor-kappaB P65) ELISA Kit

 Product Gene Name   

NF-kappaB p65 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2505513 COA    COA PDF
 OMIM    164011
 3D Structure    ModBase 3D Structure for P19838
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 Species Reactivity    Rat
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 Specificity    This kit recognizes natural and recombinant Rat NF-kappaB p65. No significant cross-reactivity or interference between Rat NF-kappaB p65 and analogues was observed.
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 Samples    Serum, plasma and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    78.125-5000pg/mL
 Sensitivity    46.875pg/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NF-kappaB p65 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NF-kappaB p65 purchase    MBS2505513 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the NF-kappaB p65, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 78.125-5000pg/mL in biological research samples containing NF-kappaB p65, with an estimated sensitivity of 46.875pg/mL. The ELISA analytical biochemical technique of the MBS2505513 kit is based on NF-kappaB p65 antibody-NF-kappaB p65 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NF-kappaB p65 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NF-kappaB p65. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NF-kappaB p65 elisa kit

   Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat NF-kappaB p65 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to NF-kappaB p65. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for NF-kappaB p65 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain NF-kappaB p65, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of NF-kappaB p65. You can calculate the concentration of NF-kappaB p65 in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of NF-kappaB p65 elisa kit    NF-kappaB p65 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2505513. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NF-kappaB p65. It may not necessarily be applicable to this product.
 NCBI GI #    34577122
 NCBI GeneID    4790
 NCBI Accession #    NP_003989.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_003998.3 [Other Products]
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 UniProt Primary Accession #    P19838 [Other Products]
 UniProt Secondary Accession #    Q68D84; Q86V43; Q8N4X7; Q9NZC0; A8K5Y5; B3KVE8 [Other Products]
 UniProt Related Accession #    P19838 [Other Products]
 Molecular Weight    85,520 Da
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 NCBI Official Full Name    nuclear factor NF-kappa-B p105 subunit isoform 1
 NCBI Official Synonym Full Names    nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
 NCBI Official Symbol    NFKB1 [Similar Products]
 NCBI Official Synonym Symbols   
p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
[Similar Products]
 NCBI Protein Information    nuclear factor NF-kappa-B p105 subunit; DNA binding factor KBF1; DNA-binding factor KBF1; NF-kappabeta; nuclear factor NF-kappa-B p50 subunit; nuclear factor kappa-B DNA binding subunit
 UniProt Protein Name    Nuclear factor NF-kappa-B p105 subunit
 UniProt Synonym Protein Names   
DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1Nuclear factor NF-kappa-B p50 subunit
 UniProt Gene Name    NFKB1 [Similar Products]
 UniProt Entry Name    NFKB1_HUMAN
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 NCBI Summary for NF-kappaB p65    This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
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 UniProt Comments for NF-kappaB p65    NFkB-p105: a transcription factor of the nuclear factor-kappaB ( NFkB) group. Undergoes cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of NFkB. NFkB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Two alternatively spliced isoforms have been described.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; heat shock protein binding; double-stranded DNA binding; chromatin binding; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; I-kappaB kinase/NF-kappaB cascade; nerve growth factor receptor signaling pathway; apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of cholesterol transport; negative regulation of cellular protein metabolic process; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 5 signaling pathway; positive regulation of interferon type I production; inflammatory response; toll-like receptor 4 signaling pathway; membrane protein intracellular domain proteolysis; MyD88-independent toll-like receptor signaling pathway; negative regulation of interleukin-12 biosynthetic process; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; response to copper ion; negative regulation of inflammatory response; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; toll-like receptor 9 signaling pathway; negative regulation of apoptosis
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 Research Articles on NF-kappaB p65    1. TLR2 and its co-receptors, TLR1 and TLR6, contribute to the pathogenicity of aPLA; aPLA are internalized via clathrin- and CD14-dependent endocytosis; and endocytosis is required for NF-kappaB activation
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NF-kappaB p65 elisa kitPathways associated with NF-kappaB p65 elisa kit
 Reference Product  PubMed Publications
 RELA elisa kit  >116 publications with NF-kappaB p65 and RELA
 IKBKB elisa kit  >47 publications with NF-kappaB p65 and IKBKB
 NFKBIA elisa kit  >35 publications with NF-kappaB p65 and NFKBIA
 CHUK elisa kit  >14 publications with NF-kappaB p65 and CHUK
 MAP3K8 elisa kit  >11 publications with NF-kappaB p65 and MAP3K8
 HDAC1 elisa kit  >6 publications with NF-kappaB p65 and HDAC1
 BCL3 elisa kit  >6 publications with NF-kappaB p65 and BCL3
 NR3C1 elisa kit  >2 publications with NF-kappaB p65 and NR3C1
 EP300 elisa kit  >1 publications with NF-kappaB p65 and EP300
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of NF-kappaB In B Cells Pathway antibodies  Activation Of NF-kappaB In B Cells Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Alternative NF-kappaB Pathway antibodies  Alternative NF-kappaB Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
Diseases associated with NF-kappaB p65 elisa kitOrgans/Tissues associated with NF-kappaB p65 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >179 publications with NF-kappaB p65 and Inflammation
 Necrosis Antibodies  >120 publications with NF-kappaB p65 and Necrosis
 Cardiovascular Diseases Antibodies  >57 publications with NF-kappaB p65 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >31 publications with NF-kappaB p65 and Disease Models, Animal
 Nervous System Diseases Antibodies  >27 publications with NF-kappaB p65 and Nervous System Diseases
 Liver Diseases Antibodies  >24 publications with NF-kappaB p65 and Liver Diseases
 Brain Diseases Antibodies  >20 publications with NF-kappaB p65 and Brain Diseases
 Heart Diseases Antibodies  >17 publications with NF-kappaB p65 and Heart Diseases
 Adenocarcinoma Antibodies  >17 publications with NF-kappaB p65 and Adenocarcinoma
 Cell Transformation, Neoplastic Antibodies  >13 publications with NF-kappaB p65 and Cell Transformation, Neoplastic
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >181 publications with NF-kappaB p65 and Blood
 Bone Antibodies  >55 publications with NF-kappaB p65 and Bone
 Vascular Antibodies  >54 publications with NF-kappaB p65 and Vascular
 Liver Antibodies  >46 publications with NF-kappaB p65 and Liver
 Lung Antibodies  >35 publications with NF-kappaB p65 and Lung
 Bone Marrow Antibodies  >33 publications with NF-kappaB p65 and Bone Marrow
 Brain Antibodies  >32 publications with NF-kappaB p65 and Brain
 Muscle Antibodies  >27 publications with NF-kappaB p65 and Muscle
 Kidney Antibodies  >27 publications with NF-kappaB p65 and Kidney
 Heart Antibodies  >21 publications with NF-kappaB p65 and Heart
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