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NOX 4 elisa kit :: Rat NOX4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2503069
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

NOX4 (NOX 4), ELISA Kit

★Popular Item★
 Also Known As   

Rat NOX4 (Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4) ELISA Kit

 Product Gene Name   

NOX 4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    605261
 3D Structure    ModBase 3D Structure for Q9NPH5
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 Species Reactivity    Rat
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 Specificity    This kit recognizes natural and recombinant Rat NOX 4. No significant cross-reactivity or interference between Rat NOX 4 and analogues was observed.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    0.313-20ng/mL
 Sensitivity    Min: 0.188ng/mL; Max: 20ng/mL
 Intended Uses    This ELISA kit applies to the in vitro quantitative determination of Rat NOX 4 concentrations in serum, plasma and other biological fluids.
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NOX 4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NOX 4 purchase    MBS2503069 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the NOX4 (NOX 4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.313-20ng/mL in biological research samples containing NOX 4, with an estimated sensitivity of Min: 0.188ng/mL; Max: 20ng/mL. The ELISA analytical biochemical technique of the MBS2503069 kit is based on NOX 4 antibody-NOX 4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NOX 4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NOX 4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NOX 4 elisa kit

   Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to NOX 4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for NOX 4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain NOX 4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of NOX 4. You can calculate the concentration of NOX 4 in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of NOX 4 elisa kit    NOX 4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2503069. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NOX 4. It may not necessarily be applicable to this product.
 NCBI GI #    219842348
 NCBI GeneID    50507
 NCBI Accession #    NP_001137309.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001143837.1 [Other Products]
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 UniProt Primary Accession #    Q9NPH5 [Other Products]
 UniProt Secondary Accession #    Q5K3R4; Q5K3R5; Q5K3R6; Q5K3R8; Q7Z7G3; Q86V92; A8K715; B7Z520; E7EMD7 [Other Products]
 UniProt Related Accession #    Q9NPH5 [Other Products]
 Molecular Weight    59,915 Da
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 NCBI Official Full Name    NADPH oxidase 4 isoform c
 NCBI Official Synonym Full Names    NADPH oxidase 4
 NCBI Official Symbol    NOX4 [Similar Products]
 NCBI Official Synonym Symbols   
KOX; KOX-1; RENOX
[Similar Products]
 NCBI Protein Information    NADPH oxidase 4; kidney oxidase-1; kidney superoxide-producing NADPH oxidase; renal NAD(P)H-oxidase
 UniProt Protein Name    NADPH oxidase 4
 UniProt Synonym Protein Names   
Kidney oxidase-1; KOX-1; Kidney superoxide-producing NADPH oxidase; Renal NAD(P)H-oxidase
 UniProt Gene Name    NOX4 [Similar Products]
 UniProt Synonym Gene Names    RENOX; KOX-1 [Similar Products]
 UniProt Entry Name    NOX4_HUMAN
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 NCBI Summary for NOX 4    This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009]
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 UniProt Comments for NOX 4    NOX4: Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 4 displays an increased activity. Isoform 5 and isoform 6 display reduced activity. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.6.3.-; Membrane protein, multi-pass; Membrane protein, integral; Nucleolus; Cell cycle regulation

Chromosomal Location of Human Ortholog: 11q14.2-q21

Cellular Component: endoplasmic reticulum membrane; focal adhesion; mitochondrion; perinuclear region of cytoplasm; apical plasma membrane; integral to membrane; stress fiber; nucleolus; NADPH oxidase complex

Molecular Function: FAD binding; electron carrier activity; oxygen sensor activity; NAD(P)H oxidase activity; superoxide-generating NADPH oxidase activity; nucleotide binding; heme binding

Biological Process: positive regulation of apoptosis; cell morphogenesis; cell aging; homocysteine metabolic process; positive regulation of smooth muscle cell migration; cardiac muscle cell differentiation; positive regulation of MAP kinase activity; positive regulation of protein kinase B signaling cascade; negative regulation of cell proliferation; positive regulation of stress fiber formation; response to hypoxia; inflammatory response; superoxide release; bone resorption
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 Research Articles on NOX 4    1. Loss of Trx-1 and upregulation of NOX4 contribute to the imbalance in the redox-status of senescent endothelial cells ex vivo and in vivo.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NOX 4 elisa kitPathways associated with NOX 4 elisa kit
 Reference Product  PubMed Publications
 TGFB1 elisa kit  >74 publications with NOX 4 and TGFB1
 CYBA elisa kit  >57 publications with NOX 4 and CYBA
 TLR4 elisa kit  >13 publications with NOX 4 and TLR4
 AKT1 elisa kit  >6 publications with NOX 4 and AKT1
 PTPN1 elisa kit  >6 publications with NOX 4 and PTPN1
 SRF elisa kit  >3 publications with NOX 4 and SRF
 VEGFA elisa kit  >3 publications with NOX 4 and VEGFA
 MMP1 elisa kit  >2 publications with NOX 4 and MMP1
 IGFBP3 elisa kit  >1 publications with NOX 4 and IGFBP3
 Products by Pathway  Pathway Diagram
 Oxidative Stress Pathway antibodies  Oxidative Stress Pathway Diagram
 Signaling Events Mediated By PTP1B Pathway antibodies  Signaling Events Mediated By PTP1B Pathway Diagram
 Spinal Cord Injury Pathway antibodies  Spinal Cord Injury Pathway Diagram
Diseases associated with NOX 4 elisa kitOrgans/Tissues associated with NOX 4 elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >258 publications with NOX 4 and Cardiovascular Diseases
 Hypertension Antibodies  >169 publications with NOX 4 and Hypertension
 Inflammation Antibodies  >159 publications with NOX 4 and Inflammation
 Disease Models, Animal Antibodies  >149 publications with NOX 4 and Disease Models, Animal
 Fibrosis Antibodies  >141 publications with NOX 4 and Fibrosis
 Heart Diseases Antibodies  >110 publications with NOX 4 and Heart Diseases
 Atherosclerosis Antibodies  >97 publications with NOX 4 and Atherosclerosis
 Kidney Diseases Antibodies  >91 publications with NOX 4 and Kidney Diseases
 Hypertrophy Antibodies  >89 publications with NOX 4 and Hypertrophy
 Necrosis Antibodies  >63 publications with NOX 4 and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >445 publications with NOX 4 and Vascular
 Kidney Antibodies  >195 publications with NOX 4 and Kidney
 Brain Antibodies  >56 publications with NOX 4 and Brain
 Connective Tissue Antibodies  >40 publications with NOX 4 and Connective Tissue
 Eye Antibodies  >32 publications with NOX 4 and Eye
 Embryonic Tissue Antibodies  >20 publications with NOX 4 and Embryonic Tissue
 Intestine Antibodies  >8 publications with NOX 4 and Intestine
 Pancreas Antibodies  >8 publications with NOX 4 and Pancreas
 Placenta Antibodies  >5 publications with NOX 4 and Placenta
 Mouth Antibodies  >4 publications with NOX 4 and Mouth
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