View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List Rat Nesfatin-1 ELISA Kit Catalog # MBS705284 Unit / Price 24-Strip-Wells (LIMIT 1) / $285 +1 FREE 8GB USB 48-Strip-Wells / $555 +1 FREE 8GB USB 96-Strip-Wells / $780 +1 FREE 8GB USB 5x96-Strip-Wells / $2,970 +3 FREE 8GB USB 10x96-Strip-Wells / $5,670 +7 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Nesfatin-1, ELISA Kit Popular Item Full Product Name Rat Nesfatin-1 ELISA Kit Product Synonym Names Rat Nesfatin-1 ELISA Kit; Nesfatin-1 Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual MBS705284 Testing Data Testing Data PDF Species Reactivity Rat Specificity This assay has high sensitivity and excellent specificity for detection of Rat. No significant cross-reactivity or interference between Rat and analogues was observed. Samples Serum, plasma, tissue homogenates Assay Type Sandwich Detection Range 15.6 pg/ml-1000 pg/ml Sensitivity 3.9 pg/ml Intra-assay Precision Intra-assay Precision (Precision within an assay): CV% is less than 8%Three samples of known concentration were tested twenty times on one plate to assess.Inter-assay Precision (Precision between assays): CV% is less than 10%Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength 450 nm Sample Volume 50-100ul Preparation and Storage Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions. ISO Certification Manufactured in an ISO 9001:2015 Certified Laboratory. Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of Nesfatin-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms for Nesfatin-1 purchase MBS705284 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Nesfatin-1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Nesfatin-1. The ELISA analytical biochemical technique of the MBS705284 kit is based on Nesfatin-1 antibody-Nesfatin-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Nesfatin-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Nesfatin-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for Nesfatin-1 elisa kit Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Nesfatin-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Nesfatin-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Nesfatin-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Nesfatin-1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Typical Testing Data/Standard Curve (for reference only) of Nesfatin-1 elisa kit TOP Sample Manual Insert of MBS705284. Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP