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NCAN elisa kit :: Rat Neurocan (NCAN) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS450382
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $425 +1 FREE 8GB USB
96-Strip-Wells  /  $570 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,595 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,040 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Neurocan (NCAN), ELISA Kit

★Popular Item★
 Also Known As   

Rat Neurocan (NCAN) ELISA Kit

 Product Synonym Names    CSPG3; NEUR; Neurocan Core Protein; Chondroitin Sulfate Proteoglycan 3; Neurocan Proteoglycan
 Product Gene Name   

NCAN elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P55067
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of NCAN. No significant cross-reactivity or interference between NCAN and analogues was observed.
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 Samples    Tissue homogenates and other biological fluids.
 Assay Type    Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    0.055ng/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NCAN were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NCAN were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NCAN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NCAN purchase    MBS450382 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Neurocan (NCAN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing NCAN, with an estimated sensitivity of 0.055ng/mL. The ELISA analytical biochemical technique of the MBS450382 kit is based on NCAN antibody-NCAN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NCAN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NCAN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NCAN elisa kit

   Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NCAN in rat tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NCAN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NCAN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NCAN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NCAN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Standard Curve/Testing Data of NCAN elisa kit    NCAN elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS450382. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NCAN. It may not necessarily be applicable to this product.
 NCBI GI #    1709256
 NCBI GeneID    58982
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 UniProt Primary Accession #    P55067 [Other Products]
 UniProt Related Accession #    P55067 [Other Products]
 Molecular Weight    135,545 Da
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 NCBI Official Full Name    Neurocan core protein
 NCBI Official Synonym Full Names    neurocan
 NCBI Official Symbol    Ncan [Similar Products]
 NCBI Official Synonym Symbols   
Cspg3
[Similar Products]
 NCBI Protein Information    neurocan core protein
 UniProt Protein Name    Neurocan core protein
 UniProt Synonym Protein Names   
245 kDa early postnatal core glycoprotein; Chondroitin sulfate proteoglycan 3
 Protein Family    Neurocan core protein
 UniProt Gene Name    Ncan [Similar Products]
 UniProt Synonym Gene Names    Cspg3 [Similar Products]
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 NCBI Summary for NCAN    chondroitin sulfate proteoglycan that may play a role in cell adhesion and cell migration [RGD, Feb 2006]
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 UniProt Comments for NCAN    May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid.
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 Research Articles on NCAN    1. In vitro results showed that MMP-2 was the dominant MMP expressed by olfactory ensheathing cells (OECs). In vivo results revealed that transplanted OECs secreted MMP-2 in injured spinal cord and that the transplantation decreased neurocan expression.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NCAN elisa kitPathways associated with NCAN elisa kit
 Reference Product  PubMed Publications
 Vcan elisa kit  >16 publications with NCAN and Vcan
 Cspg5 elisa kit  >3 publications with NCAN and Cspg5
 Sdc3 elisa kit  >2 publications with NCAN and Sdc3
 Gpc2 elisa kit  >1 publications with NCAN and Gpc2
 Dcn elisa kit  >1 publications with NCAN and Dcn
 Products by Pathway  Pathway Diagram
 A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway antibodies  A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 CS/DS Degradation Pathway antibodies  CS/DS Degradation Pathway Diagram
 Chondroitin Sulfate Biosynthesis Pathway antibodies  Chondroitin Sulfate Biosynthesis Pathway Diagram
 Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway antibodies  Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway Diagram
 Dermatan Sulfate Biosynthesis Pathway antibodies  Dermatan Sulfate Biosynthesis Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 ECM Proteoglycans Pathway antibodies  ECM Proteoglycans Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Glycosaminoglycan Metabolism Pathway antibodies  Glycosaminoglycan Metabolism Pathway Diagram
Organs/Tissues associated with NCAN elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >96 publications with NCAN and Brain
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