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NF elisa kit :: Rat Neurofilament (NF) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS266244
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $275 +1 FREE 8GB USB
96-Strip-Wells  /  $435 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Neurofilament (NF), ELISA Kit

★Popular Item★
 Also Known As   

Rat Neurofilament (NF) ELISA Kit

 Product Gene Name   

NF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    607734
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 Species Reactivity    Rat
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 Specificity    No cross-reaction with other factors.
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 Samples    Serum, plasma or cell culture supernatant
 Assay Type    Quantitative Sandwich
 Detection Range    20 ng/ml-0.312 ng/ml
 Sensitivity    0.06 ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NF purchase    MBS266244 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Neurofilament (NF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 20 ng/ml-0.312 ng/ml in biological research samples containing NF, with an estimated sensitivity of 0.06 ng/ml. The ELISA analytical biochemical technique of the MBS266244 kit is based on NF antibody-NF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma or cell culture supernatant. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NF elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat NF monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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  Typical Testing Data/Standard Curve (for reference only) of NF elisa kit    NF elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS266244. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NF. It may not necessarily be applicable to this product.
 NCBI GI #    105990539
 NCBI GeneID    4747
 NCBI Accession #    NP_006149.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_006158.4 [Other Products]
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 UniProt Secondary Accession #    Q16154; Q8IU72; B9ZVN2 [Other Products]
 UniProt Related Accession #    P07196 [Other Products]
 Molecular Weight    61,517 Da
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 NCBI Official Full Name    neurofilament light polypeptide
 NCBI Official Synonym Full Names    neurofilament, light polypeptide
 NCBI Official Symbol    NEFL [Similar Products]
 NCBI Official Synonym Symbols   
NFL; NF-L; NF68; CMT1F; CMT2E; PPP1R110
[Similar Products]
 NCBI Protein Information    neurofilament light polypeptide; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament protein, light chain; neurofilament, light polypeptide 68kDa; light molecular weight neurofilament protein; protein phosphatase 1, regulatory subunit 110
 UniProt Protein Name    Neurofilament light polypeptide
 UniProt Synonym Protein Names   
68 kDa neurofilament protein; Neurofilament triplet L protein
 UniProt Gene Name    NEFL [Similar Products]
 UniProt Synonym Gene Names    NF68; NFL; NF-L [Similar Products]
 UniProt Entry Name    NFL_HUMAN
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 NCBI Summary for NF    Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]
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 UniProt Comments for NF    NFL: one of the three (L, M, and H) intermediate filament proteins that form neurofilaments. Neurofilaments are involved in the maintenance of neuronal caliber. NF-L is the most abundant of the three neurofilament proteins. Defects cause Charcot-Marie-Tooth disease type 1F (CMT1F).

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: TSC1-TSC2 complex; growth cone; axon; cytoplasm; neurofilament; cytosol

Molecular Function: protein C-terminus binding; protein binding, bridging; protein domain specific binding; identical protein binding; protein binding; phospholipase binding; structural constituent of cytoskeleton

Biological Process: protein polymerization; response to peptide hormone stimulus; neurofilament bundle assembly; response to toxin; intermediate filament organization; microtubule cytoskeleton organization and biogenesis; axon regeneration in the peripheral nervous system; retrograde axon cargo transport; synaptic transmission; axon transport of mitochondrion; regulation of axon diameter; positive regulation of axonogenesis; negative regulation of neuron apoptosis; anterograde axon cargo transport; locomotion; neuromuscular process controlling balance; neurite morphogenesis; response to corticosterone stimulus

Disease: Charcot-marie-tooth Disease, Demyelinating, Type 1f; Charcot-marie-tooth Disease, Axonal, Type 2e
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 Research Articles on NF    1. The expression of two miRNAs (miRNAs miR-b1336 and miR-b2403) whose effect is to stabilize NEFL mRNA, are down regulated in amyotrophic lateral sclerosis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NF elisa kitPathways associated with NF elisa kit
 Reference Product  PubMed Publications
 MTMR2 elisa kit  >6 publications with NF and MTMR2
 NEFH elisa kit  >3 publications with NF and NEFH
 GAN elisa kit  >2 publications with NF and GAN
 NEFM elisa kit  >2 publications with NF and NEFM
 TSC1 elisa kit  >1 publications with NF and TSC1
 Products by Pathway  Pathway Diagram
 Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway antibodies  Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 CREB Phosphorylation Through The Activation Of CaMKII Pathway antibodies  CREB Phosphorylation Through The Activation Of CaMKII Pathway Diagram
 CREB Phosphorylation Through The Activation Of Ras Pathway antibodies  CREB Phosphorylation Through The Activation Of Ras Pathway Diagram
 Neuronal System Pathway antibodies  Neuronal System Pathway Diagram
 Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway antibodies  Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway Diagram
 Post NMDA Receptor Activation Events Pathway antibodies  Post NMDA Receptor Activation Events Pathway Diagram
 Ras Activation Uopn Ca2+ Infux Through NMDA Receptor Pathway antibodies  Ras Activation Uopn Ca2+ Infux Through NMDA Receptor Pathway Diagram
Diseases associated with NF elisa kitOrgans/Tissues associated with NF elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >53 publications with NF and Nervous System Diseases
 Polyneuropathies Antibodies  >42 publications with NF and Polyneuropathies
 Nervous System Malformations Antibodies  >42 publications with NF and Nervous System Malformations
 Brain Diseases Antibodies  >5 publications with NF and Brain Diseases
 Disease Models, Animal Antibodies  >4 publications with NF and Disease Models, Animal
 Charcot-Marie-Tooth disease, Type 1F Antibodies  >4 publications with NF and Charcot-Marie-Tooth disease, Type 1F
 Pain Antibodies  >3 publications with NF and Pain
 Lung Neoplasms Antibodies  >2 publications with NF and Lung Neoplasms
 Hypertension Antibodies  >2 publications with NF and Hypertension
 Ataxia Antibodies  >2 publications with NF and Ataxia
 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >34 publications with NF and Nerve
 Brain Antibodies  >16 publications with NF and Brain
 Prostate Antibodies  >11 publications with NF and Prostate
 Spinal Cord Antibodies  >6 publications with NF and Spinal Cord
 Lung Antibodies  >4 publications with NF and Lung
 Eye Antibodies  >3 publications with NF and Eye
 Ganglia Antibodies  >2 publications with NF and Ganglia
 Kidney Antibodies  >2 publications with NF and Kidney
 Bone Marrow Antibodies  >1 publications with NF and Bone Marrow
 Embryonic Tissue Antibodies  >1 publications with NF and Embryonic Tissue
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