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NGAL elisa kit :: Rat Neutrophil Gelatinase Associated Lipocalin (NGAL) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS260195
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $275 +1 FREE 8GB USB
96-Strip-Wells  /  $435 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Neutrophil Gelatinase Associated Lipocalin (NGAL), ELISA Kit

★Popular Item★
 Also Known As   

Rat Neutrophil Gelatinase Associated Lipocalin (NGAL) ELISA Kit

 Product Gene Name   

NGAL elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    600181
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 Species Reactivity    Rat
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 Specificity    No cross-reaction with other factors.
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 Samples    Serum, plasma or cell culture supernatant
 Assay Type    Quantitative Sandwich
 Detection Range    2000 pg/ml-31.2 pg/ml
 Sensitivity    Up to 12 pg/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NGAL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NGAL purchase    MBS260195 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Neutrophil Gelatinase Associated Lipocalin (NGAL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 2000 pg/ml-31.2 pg/ml in biological research samples containing NGAL, with an estimated sensitivity of Up to 12 pg/ml. The ELISA analytical biochemical technique of the MBS260195 kit is based on NGAL antibody-NGAL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NGAL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NGAL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma or cell culture supernatant. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for NGAL elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat NGAL monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Standard Curve/Testing Data of NGAL elisa kit    NGAL elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS260195. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NGAL. It may not necessarily be applicable to this product.
 NCBI GI #    38455402
 NCBI GeneID    3934
 NCBI Accession #    NP_005555.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_005564.3 [Other Products]
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 UniProt Secondary Accession #    P30150; Q5SYV9; Q5SYW0; Q6FGL5; Q92683; A6NII8; B4DWV4; B7ZAA2 [Other Products]
 UniProt Related Accession #    P80188 [Other Products]
 Molecular Weight    22,457 Da
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 NCBI Official Full Name    neutrophil gelatinase-associated lipocalin
 NCBI Official Synonym Full Names    lipocalin 2
 NCBI Official Symbol    LCN2 [Similar Products]
 NCBI Official Synonym Symbols   
24p3; MSFI; NGAL
[Similar Products]
 NCBI Protein Information    neutrophil gelatinase-associated lipocalin; p25; lipocalin-2; oncogene 24p3; siderocalin LCN2; migration-stimulating factor inhibitor; 25 kDa alpha-2-microglobulin-related subunit of MMP-9
 UniProt Protein Name    Neutrophil gelatinase-associated lipocalin
 UniProt Synonym Protein Names   
25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; Oncogene 24p3; Siderocalin LCN2; p25
 UniProt Gene Name    LCN2 [Similar Products]
 UniProt Synonym Gene Names    HNL; NGAL; NGAL [Similar Products]
 UniProt Entry Name    NGAL_HUMAN
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 UniProt Comments for NGAL    LCN2: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. Belongs to the calycin superfamily. Lipocalin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: extracellular space; extracellular region; cytosol

Molecular Function: protein homodimerization activity; protease binding; transporter activity; iron ion binding

Biological Process: response to drug; cellular response to nutrient levels; cellular iron ion homeostasis; response to virus; positive regulation of cell projection organization and biogenesis; siderophore transport; innate immune response; ion transport; response to herbicide; transmembrane transport
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 Research Articles on NGAL    1. NGAL, IL-18, KIM-1, L-FABP, and albumin differed between etiologies and were significantly higher in patients adjudicated with acute kidney injury.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NGAL elisa kitDiseases associated with NGAL elisa kit
 Reference Product  PubMed Publications
 EGFR elisa kit  >49 publications with NGAL and EGFR
 HMOX1 elisa kit  >10 publications with NGAL and HMOX1
 MMP9 elisa kit  >10 publications with NGAL and MMP9
 PPARG elisa kit  >6 publications with NGAL and PPARG
 LIF elisa kit  >2 publications with NGAL and LIF
 GFAP elisa kit  >1 publications with NGAL and GFAP
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >417 publications with NGAL and Kidney Diseases
 Acute Kidney Injury Antibodies  >384 publications with NGAL and Acute Kidney Injury
 Renal Insufficiency Antibodies  >294 publications with NGAL and Renal Insufficiency
 Inflammation Antibodies  >201 publications with NGAL and Inflammation
 Neoplasms Antibodies  >156 publications with NGAL and Neoplasms
 Cardiovascular Diseases Antibodies  >142 publications with NGAL and Cardiovascular Diseases
 Heart Diseases Antibodies  >71 publications with NGAL and Heart Diseases
 Necrosis Antibodies  >70 publications with NGAL and Necrosis
 Disease Models, Animal Antibodies  >62 publications with NGAL and Disease Models, Animal
 Proteinuria Antibodies  >52 publications with NGAL and Proteinuria
Organs/Tissues associated with NGAL elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >552 publications with NGAL and Blood
 Kidney Antibodies  >487 publications with NGAL and Kidney
 Liver Antibodies  >102 publications with NGAL and Liver
 Lung Antibodies  >62 publications with NGAL and Lung
 Bone Antibodies  >31 publications with NGAL and Bone
 Connective Tissue Antibodies  >26 publications with NGAL and Connective Tissue
 Muscle Antibodies  >25 publications with NGAL and Muscle
 Bone Marrow Antibodies  >23 publications with NGAL and Bone Marrow
 Intestine Antibodies  >19 publications with NGAL and Intestine
 Uterus Antibodies  >16 publications with NGAL and Uterus
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