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NET elisa kit :: Rat Norepinephrine Transporter (NET) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2023721 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $285 +1 FREE 8GB USB
48-Strip-Wells  /  $530 +1 FREE 8GB USB
96-Strip-Wells  /  $715 +1 FREE 8GB USB
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10x96-Strip-Wells  /  $5,280 +6 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Norepinephrine Transporter (NET), ELISA Kit

 Also Known As   

Norepinephrine Transporter (NET) ELISA Kit

 Product Synonym Names    SLC6A2; NAT1; NET; NET1; Sodium-dependent noradrenaline transporter; Noradrenaline Transporter; Solute Carrier Family 6 member 2; Neurotransmitter transporter; Noradrenalin
 Product Gene Name   

NET elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    163970
 3D Structure    ModBase 3D Structure for P23975
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Norepinephrine Transporter (NET).
No significant cross-reactivity or interference between Norepinephrine Transporter (NET) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates, Cell lysates and Other Biological Fluids
 Detection Range    0.312-20ng/mL
 Sensitivity    < 0.112ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Norepinephrine Transporter (NET) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Norepinephrine Transporter (NET) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NET elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NET purchase    MBS2023721 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Norepinephrine Transporter (NET) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20ng/mL in biological research samples containing NET, with an estimated sensitivity of < 0.112ng/mL. The ELISA analytical biochemical technique of the MBS2023721 kit is based on NET antibody-NET antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NET antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NET. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NET elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Norepinephrine Transporter (NET). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Norepinephrine Transporter (NET). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Norepinephrine Transporter (NET), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Norepinephrine Transporter (NET) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for NET elisa kit    Endocrinology
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 Typical Testing Data/Standard Curve (for reference only) of NET elisa kit    NET elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2023721. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NET. It may not necessarily be applicable to this product.
 NCBI GI #    1143479
 NCBI GeneID    6530
 NCBI Accession #    CAA62566.1 [Other Products]
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 UniProt Primary Accession #    P23975 [Other Products]
 UniProt Secondary Accession #    Q96KH8; B2R707; B4DX48 [Other Products]
 UniProt Related Accession #    P23975 [Other Products]
 Molecular Weight    57,370 Da
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 NCBI Official Full Name    norepinephrine transporter
 NCBI Official Synonym Full Names    solute carrier family 6 (neurotransmitter transporter), member 2
 NCBI Official Symbol    SLC6A2 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    sodium-dependent noradrenaline transporter
 UniProt Protein Name    Sodium-dependent noradrenaline transporter
 UniProt Synonym Protein Names   
Norepinephrine transporter; NET; Solute carrier family 6 member 2
 UniProt Gene Name    SLC6A2 [Similar Products]
 UniProt Synonym Gene Names    NAT1; NET1; SLC6A5; NET [Similar Products]
 UniProt Entry Name    SC6A2_HUMAN
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 NCBI Summary for NET    This gene encodes a member of the sodium:neurotransmitter symporter family. This member is a multi-pass membrane protein, which is responsible for reuptake of norepinephrine into presynaptic nerve terminals and is a regulator of norepinephrine homeostasis. Mutations in this gene cause orthostatic intolerance, a syndrome characterized by lightheadedness, fatigue, altered mentation and syncope. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]
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 UniProt Comments for NET    SLC6A2: Amine transporter. Terminates the action of noradrenaline by its high affinity sodium-dependent reuptake into presynaptic terminals. Defects in SLC6A2 are a cause of orthostatic intolerance (OI). OI is a syndrome characterized by lightheadedness, fatigue, altered mentation and syncope. It is associated with postural tachycardia. Plasma norepinephrine concentration is abnormally high. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A2 subfamily.

Protein type: Membrane protein, integral; Transporter; Membrane protein, multi-pass; Transporter, SLC family

Chromosomal Location of Human Ortholog: 16q12.2

Cellular Component: neuron projection; cell surface; membrane; integral to plasma membrane; plasma membrane; lipid raft

Molecular Function: protein binding; beta-tubulin binding; monoamine transmembrane transporter activity; alpha-tubulin binding; norephinephrine:sodium symporter activity

Biological Process: response to drug; synaptic transmission; norepinephrine transport; neurotransmitter transport; transport; monoamine transport; response to pain; transmembrane transport

Disease: Orthostatic Intolerance
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 Research Articles on NET    1. A Cox regression analysis for remission incidence during the 8-week treatment course significantly depends on SLC6A2 variants (rs28386840, rs40434, and rs187714)from major depression after venlafaxine treatment.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NET elisa kitPathways associated with NET elisa kit
 Reference Product  PubMed Publications
 SLC18A2 elisa kit  >13 publications with NET and SLC18A2
 PICK1 elisa kit  >2 publications with NET and PICK1
 STX1A elisa kit  >1 publications with NET and STX1A
 Products by Pathway  Pathway Diagram
 Monoamine Transport Pathway antibodies  Monoamine Transport Pathway Diagram
 Na+/Cl- Dependent Neurotransmitter Transporters Pathway antibodies  Na+/Cl- Dependent Neurotransmitter Transporters Pathway Diagram
 SLC-mediated Transmembrane Transport Pathway antibodies  SLC-mediated Transmembrane Transport Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram
 Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway antibodies  Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway Diagram
Diseases associated with NET elisa kitOrgans/Tissues associated with NET elisa kit
 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >124 publications with NET and Mental Disorders
 Attention Deficit Disorder with Hyperactivity Antibodies  >51 publications with NET and Attention Deficit Disorder with Hyperactivity
 Nervous System Diseases Antibodies  >50 publications with NET and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >48 publications with NET and Cardiovascular Diseases
 Depressive Disorder Antibodies  >38 publications with NET and Depressive Disorder
 Heart Diseases Antibodies  >32 publications with NET and Heart Diseases
 Brain Diseases Antibodies  >31 publications with NET and Brain Diseases
 Disease Models, Animal Antibodies  >26 publications with NET and Disease Models, Animal
 Hypertension Antibodies  >26 publications with NET and Hypertension
 Tachycardia Antibodies  >17 publications with NET and Tachycardia
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >279 publications with NET and Brain
 Nerve Antibodies  >267 publications with NET and Nerve
 Muscle Antibodies  >44 publications with NET and Muscle
 Kidney Antibodies  >28 publications with NET and Kidney
 Placenta Antibodies  >18 publications with NET and Placenta
 Intestine Antibodies  >2 publications with NET and Intestine
 Testis Antibodies  >2 publications with NET and Testis
 Prostate Antibodies  >1 publications with NET and Prostate
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