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NFkB elisa kit :: Rat Nuclear Factor Kappa B (NFkB) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS453975
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $550 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,485 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,830 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Nuclear Factor Kappa B (NFkB), ELISA Kit

★Popular Item★
 Also Known As   

Rat Nuclear Factor Kappa B (NFkB) ELISA Kit

 Product Synonym Names    NF-KB1; EBP-1; KBF1; NF-Kappa-B; NFKB-P105; NFKB-P50; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear factor NF-kappa-B p105 subunit
 Product Gene Name   

NFkB elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q63369
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of NFkB. No significant cross-reactivity or interference between NFkB and analogues was observed.
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 Samples    Tissue homogenates, cell lysates and other biological fluids.
 Assay Type    Sandwich
 Detection Range    0.312-20ng/mL
 Sensitivity    0.119ng/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NFkB were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NFkB were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NFkB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NFkB purchase    MBS453975 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Nuclear Factor Kappa B (NFkB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20ng/mL in biological research samples containing NFkB, with an estimated sensitivity of 0.119ng/mL. The ELISA analytical biochemical technique of the MBS453975 kit is based on NFkB antibody-NFkB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NFkB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NFkB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, cell lysates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for NFkB elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NFkB in rat tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NFkB. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NFkB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NFkB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NFkB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of NFkB elisa kit    NFkB elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS453975. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NFkB. It may not necessarily be applicable to this product.
 NCBI GI #    2500134
 NCBI GeneID    81736
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 UniProt Primary Accession #    Q63369 [Other Products]
 UniProt Related Accession #    Q63369 [Other Products]
 Molecular Weight    56,554 Da
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 NCBI Official Full Name    Nuclear factor NF-kappa-B p105 subunit
 NCBI Official Synonym Full Names    nuclear factor kappa B subunit 1
 NCBI Official Symbol    Nfkb1 [Similar Products]
 NCBI Official Synonym Symbols   
EBP-1; NF-kB
[Similar Products]
 NCBI Protein Information    nuclear factor NF-kappa-B p105 subunit
 UniProt Protein Name    Nuclear factor NF-kappa-B p105 subunit
 UniProt Synonym Protein Names   
DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
 UniProt Gene Name    Nfkb1 [Similar Products]
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 NCBI Summary for NFkB    gene encodes a 105 kD protein that can be processed to create a 50 kD protein; the larger protein is a Rel protein-specific transcription inhibitor and the smaller protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex [RGD, Feb 2006]
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 UniProt Comments for NFkB    NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. Plays a role in the regulation of apoptosis. Isoform 5, isoform 6 and isoform 7 act as inhibitors of transactivation of p50 NF-kappa-B subunit, probably by sequestering it in the cytoplasm. Isoform 3 (p98) (but not p84 or p105) acts as a transactivator of NF-kappa-B-regulated gene expression. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105 ().
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 Research Articles on NFkB    1. Remote ischemic postconditioning might protect ischemic brain against ischemia/reperfusion injury in rats by suppressing the TLR4/NF-kB pathway.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NFkB elisa kitPathways associated with NFkB elisa kit
 Reference Product  PubMed Publications
 Rela elisa kit  >153 publications with NFkB and Rela
 Tnf elisa kit  >137 publications with NFkB and Tnf
 Nfkbia elisa kit  >92 publications with NFkB and Nfkbia
 Ikbkb elisa kit  >52 publications with NFkB and Ikbkb
 Il6 elisa kit  >47 publications with NFkB and Il6
 Il1b elisa kit  >37 publications with NFkB and Il1b
 Chuk elisa kit  >16 publications with NFkB and Chuk
 Map3k8 elisa kit  >12 publications with NFkB and Map3k8
 Bcl3 elisa kit  >7 publications with NFkB and Bcl3
 Products by Pathway  Pathway Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of NF-kappaB In B Cells Pathway antibodies  Activation Of NF-kappaB In B Cells Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
Organs/Tissues associated with NFkB elisa kit
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >60 publications with NFkB and Liver
 Lung Antibodies  >50 publications with NFkB and Lung
 Brain Antibodies  >43 publications with NFkB and Brain
 Heart Antibodies  >27 publications with NFkB and Heart
 Spleen Antibodies  >21 publications with NFkB and Spleen
 Embryonic Tissue Antibodies  >9 publications with NFkB and Embryonic Tissue
 Pancreas Antibodies  >8 publications with NFkB and Pancreas
 Thymus Antibodies  >6 publications with NFkB and Thymus
 Placenta Antibodies  >6 publications with NFkB and Placenta
 Ovary Antibodies  >2 publications with NFkB and Ovary
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