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OPALIN elisa kit :: Rat Opalin (OPALIN) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7245210
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
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 Product Name   

Opalin (OPALIN), ELISA Kit

★Popular Item★
 Also Known As   

Rat Opalin (OPALIN) ELISA Kit

 Product Gene Name   

OPALIN elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of OPALIN. No significant cross-reactivity or interference between OPALIN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between OPALIN and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Competitive (Quantitative)
 Sensitivity    0.1 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of OPALIN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for OPALIN purchase    MBS7245210 is a ready-to-use microwell, strip plate Competitive (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Opalin (OPALIN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing OPALIN, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS7245210 kit is based on OPALIN antibody-OPALIN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OPALIN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OPALIN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for OPALIN elisa kit

   Intended Uses: This OPALIN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat OPALIN. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: OPALIN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-OPALIN antibody and an OPALIN-HRP conjugate. The assay sample and buffer are incubated together with OPALIN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OPALIN concentration since OPALIN from samples and OPALIN-HRP conjugate compete for the anti-OPALIN antibody binding site. Since the number of sites is limited, as more sites are occupied by OPALIN from the sample, fewer sites are left to bind OPALIN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OPALIN concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for OPALIN elisa kit    Cell Biology
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 Typical Testing Data/Standard Curve (for reference only) of OPALIN elisa kit    OPALIN elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS7245210. Click to request current manual
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NCBI/Uniprot data below describe general gene information for OPALIN. It may not necessarily be applicable to this product.
 NCBI GI #    23943844
 NCBI GeneID    226115
 NCBI Accession #    NP_705740.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_153520.1 [Other Products]
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 UniProt Secondary Accession #    Q6P903 [Other Products]
 UniProt Related Accession #    Q7M750 [Other Products]
 Molecular Weight    15,833 Da
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 NCBI Official Full Name    opalin
 NCBI Official Synonym Full Names    oligodendrocytic myelin paranodal and inner loop protein
 NCBI Official Symbol    Opalin [Similar Products]
 NCBI Official Synonym Symbols   
Tmp10; Tmem10
[Similar Products]
 NCBI Protein Information    opalin; transmembrane protein 10; transmembrane protein TMP10
 UniProt Protein Name    Opalin
 UniProt Synonym Protein Names   
Oligodendrocytic myelin paranodal and inner loop protein; Transmembrane protein 10
 Protein Family    Opalin
 UniProt Gene Name    Opalin [Similar Products]
 UniProt Synonym Gene Names    Tmem10 [Similar Products]
 UniProt Entry Name    OPALI_MOUSE
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 UniProt Comments for OPALIN    OPALIN: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: Golgi apparatus; membrane; integral to membrane; plasma membrane
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 Research Articles on OPALIN    1. study demonstrates that Tmem10 can be used as a specific marker for myelinating oligodendrocytes and perhaps for the evaluation of myelination diseases, such as multiple sclerosis
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with OPALIN elisa kitOrgans/Tissues associated with OPALIN elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >4 publications with OPALIN and Nervous System Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >10 publications with OPALIN and Brain
 Spinal Cord Antibodies  >2 publications with OPALIN and Spinal Cord
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