View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Add To Compare List OXA elisa kit :: Rat Orexin A (OXA) ELISA Kit Catalog # MBS453646 Unit / Price DISCONTINUEDClick here to view our similar product(s) OR click here to contact our technical support team for suitable replacement. Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Orexin A (OXA), ELISA Kit Popular Item Full Product Name Rat Orexin A (OXA) ELISA Kit Product Synonym Names Hypocretin-1 Product Gene Name OXA elisa kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual Species Reactivity Rat Specificity This assay has high sensitivity and excellent specificity for detection of OXA. No significant cross-reactivity or interference between OXA and analogues was observed. Samples Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay Type Quantitative Competitive Detection Range 15.62-1000pg/mL Sensitivity <4.95pg/mL Intra-assay Precision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level OXA were tested 20 times on one plate, respectively. Intra-Assay: CV<10% Inter-assay Precision Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level OXA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12% Preparation and Storage For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C. ISO Certification Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory. Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of OXA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms for OXA purchase MBS453646 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Orexin A (OXA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing OXA. The ELISA analytical biochemical technique of the MBS453646 kit is based on OXA antibody-OXA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OXA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OXA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for OXA elisa kit Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of OXA in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to OXA has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled OXA and unlabeled OXA (Standards or samples) with the pre-coated antibody specific to OXA. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of OXA in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of OXA in the sample. Typical Testing Data/Standard Curve (for reference only) of OXA elisa kit TOP Sample Manual Insert of MBS453646. Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP