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OC elisa kit :: Rat Osteocalcin (OC) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2701838 (SPECIAL PROMOTIONAL PRICING for a limited time)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $220 +1 FREE 8GB USB
48-Strip-Wells  /  $350 +1 FREE 8GB USB
96-Strip-Wells  /  $470 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,960 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,625 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Osteocalcin (OC), ELISA Kit

★Popular Item★
 Also Known As   

Rat Osteocalcin (OC) ELISA Kit

 Product Synonym Names    BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein
 Product Gene Name   

OC elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    X53698 mRNA
 3D Structure    ModBase 3D Structure for P02818
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Osteocalcin (OC).
No significant cross-reactivity or interference between Osteocalcin (OC) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue Homogenates, Cell Lysates and Other Biological Fluids
 Detection Range    15.6-1000pg/mL
 Sensitivity    < 6.3pg/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Osteocalcin (OC) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Osteocalcin (OC) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of OC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for OC purchase    MBS2701838 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Osteocalcin (OC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6-1000pg/mL in biological research samples containing OC, with an estimated sensitivity of < 6.3pg/mL. The ELISA analytical biochemical technique of the MBS2701838 kit is based on OC antibody-OC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenates, Cell Lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for OC elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteocalcin (OC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Osteocalcin (OC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Osteocalcin (OC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Osteocalcin (OC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of OC elisa kit    OC elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2701838. Click to request current manual
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NCBI/Uniprot data below describe general gene information for OC. It may not necessarily be applicable to this product.
 NCBI GI #    40316933
 NCBI GeneID    632
 NCBI Accession #    NP_954642.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_199173.5 [Other Products]
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 UniProt Primary Accession #    P02818 [Other Products]
 UniProt Secondary Accession #    Q5TCK6 [Other Products]
 UniProt Related Accession #    P02818 [Other Products]
 Molecular Weight    10,963 Da
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 NCBI Official Full Name    osteocalcin preproprotein
 NCBI Official Synonym Full Names    bone gamma-carboxyglutamate protein
 NCBI Official Symbol    BGLAP [Similar Products]
 NCBI Official Synonym Symbols   
OC; BGP; OCN
[Similar Products]
 NCBI Protein Information    osteocalcin
 UniProt Protein Name    Osteocalcin
 UniProt Synonym Protein Names   
Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein
 UniProt Gene Name    BGLAP [Similar Products]
 UniProt Synonym Gene Names    BGP [Similar Products]
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 NCBI Summary for OC    This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015]
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 UniProt Comments for OC    Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
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 Research Articles on OC    1. Demonstrate a positive association between osteocalcin, a factor known to regulate bone remodelling, with cognitive performance in older non-demented women.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with OC elisa kitPathways associated with OC elisa kit
 Reference Product  PubMed Publications
 RUNX2 elisa kit  >27 publications with OC and RUNX2
 BMP2 elisa kit  >9 publications with OC and BMP2
 TGFB1 elisa kit  >3 publications with OC and TGFB1
 VDR elisa kit  >3 publications with OC and VDR
 LEP elisa kit  >1 publications with OC and LEP
 PTHLH elisa kit  >1 publications with OC and PTHLH
 PTH elisa kit  >1 publications with OC and PTH
 ATF4 elisa kit  >1 publications with OC and ATF4
 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 FGF Signaling Pathway antibodies  FGF Signaling Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram
 Interleukin-11 Signaling Pathway antibodies  Interleukin-11 Signaling Pathway Diagram
 M Phase Pathway antibodies  M Phase Pathway Diagram
 Mitotic Anaphase Pathway antibodies  Mitotic Anaphase Pathway Diagram
 Mitotic Metaphase And Anaphase Pathway antibodies  Mitotic Metaphase And Anaphase Pathway Diagram
 Mitotic Prometaphase Pathway antibodies  Mitotic Prometaphase Pathway Diagram
 Notch-mediated HES/HEY Network Pathway antibodies  Notch-mediated HES/HEY Network Pathway Diagram
Diseases associated with OC elisa kitOrgans/Tissues associated with OC elisa kit
 Disease Name  Pubmed Publications
 Bone Diseases Antibodies  >8 publications with OC and Bone Diseases
 Osteoporosis Antibodies  >5 publications with OC and Osteoporosis
 Neoplasms Antibodies  >4 publications with OC and Neoplasms
 Hemorrhage Antibodies  >1 publications with OC and Hemorrhage
 Pain Antibodies  >1 publications with OC and Pain
 Hypertension Antibodies  >1 publications with OC and Hypertension
 Cardiovascular Diseases Antibodies  >1 publications with OC and Cardiovascular Diseases
 Inflammation Antibodies  >1 publications with OC and Inflammation
 Necrosis Antibodies  >1 publications with OC and Necrosis
 Brain Injuries Antibodies  >1 publications with OC and Brain Injuries
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >49 publications with OC and Bone
 Brain Antibodies  >3 publications with OC and Brain
 Parathyroid Antibodies  >1 publications with OC and Parathyroid
 Prostate Antibodies  >1 publications with OC and Prostate
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