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ON elisa kit :: Rat Osteonectin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS723551
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells-(Competitive)  /  $455 +1 FREE 8GB USB
48-Strip-Wells-(Sandwich)  /  $455 +1 FREE 8GB USB
96-Strip-Wells-(Competitive)  /  $660 +1 FREE 8GB USB
96-Strip-Wells-(Sandwich)  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells-(Competitive)  /  $2,925 +3 FREE 8GB USB
5x96-Strip-Wells-(Sandwich)  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells-(Competitive)  /  $5,490 +6 FREE 8GB USB
10x96-Strip-Wells-(Sandwich)  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Osteonectin, ELISA Kit

 Also Known As   

Rat Osteonectin ELISA Kit

 Product Gene Name   

ON elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    182120
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 Species Reactivity    Rat
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive or Sandwich
 Detection Range    1.0-25ng/mL
 Sensitivity    0.1ng/ml
 Intended Uses    This ON ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ON. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ON elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ON purchase    MBS723551 is a ready-to-use microwell, strip plate Competitive or Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Osteonectin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.0-25ng/mL in biological research samples containing ON, with an estimated sensitivity of 0.1ng/ml. The ELISA analytical biochemical technique of the MBS723551 kit is based on ON antibody-ON antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ON antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ON. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ON elisa kit

   Principle of the Assay: ON ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ON. Standards or samples are then added to the microtiter plate wells and ON if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ON present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for ON are added to each well to "sandwich" the ON immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ON and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ON concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for ON elisa kit    Cardiovascular
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 Typical Testing Data/Standard Curve (for reference only) of ON elisa kit    ON elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS723551. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ON. It may not necessarily be applicable to this product.
 NCBI GI #    338325
 NCBI GeneID    6678
 NCBI Accession #    AAA60993.1 [Other Products]
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 UniProt Secondary Accession #    Q6IBK4; D3DQH9 [Other Products]
 UniProt Related Accession #    P09486 [Other Products]
 Molecular Weight    34,632 Da
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 NCBI Official Full Name    osteonectin
 NCBI Official Synonym Full Names    secreted protein, acidic, cysteine-rich (osteonectin)
 NCBI Official Symbol    SPARC [Similar Products]
 NCBI Official Synonym Symbols   
ON
[Similar Products]
 NCBI Protein Information    SPARC; BM-40; osteonectin; cysteine-rich protein; basement-membrane protein 40; secreted protein acidic and rich in cysteine
 UniProt Protein Name    SPARC
 UniProt Synonym Protein Names   
Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine
 UniProt Gene Name    SPARC [Similar Products]
 UniProt Synonym Gene Names    ON; BM-40; ON [Similar Products]
 UniProt Entry Name    SPRC_HUMAN
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 NCBI Summary for ON    This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. [provided by RefSeq, Dec 2011]
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 UniProt Comments for ON    SPARC: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. Belongs to the SPARC family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5q31.3-q32

Cellular Component: extracellular space; platelet alpha granule membrane; cell surface; nuclear matrix; mitochondrion; cytoplasm; plasma membrane; extracellular region; basement membrane

Molecular Function: collagen binding; protein binding; extracellular matrix binding; calcium ion binding

Biological Process: response to gravity; receptor-mediated endocytosis; platelet activation; response to peptide hormone stimulus; extracellular matrix organization and biogenesis; ossification; response to cAMP; heart development; response to glucocorticoid stimulus; response to lipopolysaccharide; signal transduction; response to L-ascorbic acid; negative regulation of angiogenesis; response to cadmium ion; response to ethanol; platelet degranulation; response to cytokine stimulus; response to lead ion; negative regulation of endothelial cell proliferation; regulation of cell morphogenesis; blood coagulation; response to calcium ion; inner ear development; lung development

Disease: Osteogenesis Imperfecta, Type Xvii
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 Research Articles on ON    1. Down-regulated expression of ET3 attenuates the malignant behaviors of human melanoma cells partially by decreasing the expression of SPARC.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ON elisa kitPathways associated with ON elisa kit
 Reference Product  PubMed Publications
 TGFB1 elisa kit  >71 publications with ON and TGFB1
 COL1A2 elisa kit  >31 publications with ON and COL1A2
 COL1A1 elisa kit  >30 publications with ON and COL1A1
 EGF elisa kit  >24 publications with ON and EGF
 SERPINE1 elisa kit  >17 publications with ON and SERPINE1
 FN1 elisa kit  >13 publications with ON and FN1
 TIMP1 elisa kit  >6 publications with ON and TIMP1
 PLG elisa kit  >2 publications with ON and PLG
 ALB elisa kit  >1 publications with ON and ALB
 Products by Pathway  Pathway Diagram
 Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies  Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
 ECM Proteoglycans Pathway antibodies  ECM Proteoglycans Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram
 Scavenging By Class H Receptors Pathway antibodies  Scavenging By Class H Receptors Pathway Diagram
 Senescence And Autophagy Pathway antibodies  Senescence And Autophagy Pathway Diagram
 TGF-beta Receptor Signaling Pathway antibodies  TGF-beta Receptor Signaling Pathway Diagram
Diseases associated with ON elisa kitOrgans/Tissues associated with ON elisa kit
 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >110 publications with ON and Adenocarcinoma
 Disease Models, Animal Antibodies  >103 publications with ON and Disease Models, Animal
 Inflammation Antibodies  >101 publications with ON and Inflammation
 Fibrosis Antibodies  >94 publications with ON and Fibrosis
 Brain Diseases Antibodies  >76 publications with ON and Brain Diseases
 Breast Neoplasms Antibodies  >67 publications with ON and Breast Neoplasms
 Necrosis Antibodies  >60 publications with ON and Necrosis
 Cell Transformation, Neoplastic Antibodies  >48 publications with ON and Cell Transformation, Neoplastic
 Neoplasms, Experimental Antibodies  >48 publications with ON and Neoplasms, Experimental
 Kidney Diseases Antibodies  >45 publications with ON and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >1061 publications with ON and Bone
 Connective Tissue Antibodies  >440 publications with ON and Connective Tissue
 Blood Antibodies  >328 publications with ON and Blood
 Vascular Antibodies  >293 publications with ON and Vascular
 Bone Marrow Antibodies  >198 publications with ON and Bone Marrow
 Muscle Antibodies  >176 publications with ON and Muscle
 Embryonic Tissue Antibodies  >171 publications with ON and Embryonic Tissue
 Mouth Antibodies  >156 publications with ON and Mouth
 Brain Antibodies  >152 publications with ON and Brain
 Eye Antibodies  >119 publications with ON and Eye
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