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Add To Compare List OPN elisa kit :: Rat Osteopontin ELISA Kit Catalog # MBS760186 Unit / Price 48-Strip-Wells / $350 +1 FREE 8GB USB 96-Strip-Wells / $465 +1 FREE 8GB USB 5x96-Strip-Wells / $2,090 +2 FREE 8GB USB 10x96-Strip-Wells / $3,995 +4 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Osteopontin (OPN), ELISA Kit Popular Item Full Product Name Rat Osteopontin ELISA Kit Product Synonym Names OPN (Osteopontin)/Eta-1/Spp1/BNSP/Bone sialoprotein 1/MGC110940/Nephropontin/secreted phosphoprotein-1 (osteopontin; bone sialoprotein)/SPP-1/SPP1/CALPHA1 fusion/Urinary stone protein/uropontin Product Gene Name OPN elisa kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual MBS760186 COA COA PDF OMIM 166490 Species Reactivity Rat Specificity This assay has high sensitivity and excellent specificity for detection of OPN. No significant cross-reactivity or interference between OPN and analogues was observed. Assay Type Sandwich ELISA, Double Antibody Samples Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples Detection Range 39.063-2500pg/ml Sensitivity 23.438pg/ml Intra-assay Precision CV<8% Inter-assay Precision CV<10% Preparation and Storage Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C ISO Certification Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory. Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of OPN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms for OPN purchase MBS760186 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Osteopontin (OPN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing OPN. The ELISA analytical biochemical technique of the MBS760186 kit is based on OPN antibody-OPN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OPN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OPN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for OPN elisa kit Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated. Typical Testing Data/Standard Curve (for reference only) of OPN elisa kit TOP Sample Manual Insert of MBS760186. NCBI/Uniprot data below describe general gene information for OPN. It may not necessarily be applicable to this product. NCBI GI # 189405 NCBI GeneID 6696 NCBI Accession # AAA59974.1 [Other Products] UniProt Secondary Accession # Q15681; Q15682; Q15683; Q4W597; Q567T5; Q8NBK2; Q96IZ1; B2RDA1[Other Products] UniProt Related Accession # P10451[Other Products] Molecular Weight 33,843 Da TOP NCBI Official Full Name osteopontin NCBI Official Synonym Full Names secreted phosphoprotein 1 NCBI Official Symbol SPP1 [Similar Products] NCBI Official Synonym Symbols OPN; BNSP; BSPI; ETA-1 [Similar Products] NCBI Protein Information osteopontin UniProt Protein Name Osteopontin UniProt Synonym Protein Names Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin Protein Family Osteopontin UniProt Gene Name SPP1 [Similar Products] UniProt Synonym Gene Names BNSP; OPN; SPP-1 [Similar Products] UniProt Entry Name OSTP_HUMAN TOP NCBI Summary for OPN The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] TOP UniProt Comments for OPN osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing.Protein type: Secreted, signal peptide; SecretedChromosomal Location of Human Ortholog: 4q22.1Cellular Component: apical part of cell; cell projection; extracellular region; extracellular space; perinuclear region of cytoplasmMolecular Function: cytokine activity; extracellular matrix binding; protein bindingBiological Process: biomineral formation; cell adhesion; decidualization; embryo implantation; extracellular matrix disassembly; extracellular matrix organization and biogenesis; inflammatory response; negative regulation of collateral sprouting of intact axon in response to injury; neutrophil chemotaxis; osteoblast differentiation; positive regulation of bone resorption; response to steroid hormone stimulus; response to vitamin D Research Articles on OPN 1. OPN may be involved in risk organ dissemination and poor prognosis of Langerhans cell histiocytosis through the function as inflammatory cytokine/chemokine. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP Products associated with OPN elisa kit Reference Product PubMed Publications RUNX2 elisa kit >140 publications with OPN and RUNX2 CD44 elisa kit >130 publications with OPN and CD44 BMP2 elisa kit >76 publications with OPN and BMP2 THBS1 elisa kit >14 publications with OPN and THBS1 MMP7 elisa kit >11 publications with OPN and MMP7 ACP5 elisa kit >6 publications with OPN and ACP5 TP53 elisa kit >4 publications with OPN and TP53 ITGB1 elisa kit >2 publications with OPN and ITGB1 ITGAV elisa kit >2 publications with OPN and ITGAV Pathways associated with OPN elisa kit Products by Pathway Pathway Diagram BDNF Signaling Pathway antibodies BDNF Signaling Pathway Diagram Degradation Of The Extracellular Matrix Pathway antibodies Degradation Of The Extracellular Matrix Pathway Diagram Direct P53 Effectors Pathway antibodies Direct P53 Effectors Pathway Diagram ECM-receptor Interaction Pathway antibodies ECM-receptor Interaction Pathway Diagram ECM-receptor Interaction Pathway antibodies ECM-receptor Interaction Pathway Diagram Endochondral Ossification Pathway antibodies Endochondral Ossification Pathway Diagram Extracellular Matrix Organization Pathway antibodies Extracellular Matrix Organization Pathway Diagram FGF Signaling Pathway antibodies FGF Signaling Pathway Diagram Focal Adhesion Pathway antibodies Focal Adhesion Pathway Diagram Focal Adhesion Pathway antibodies Focal Adhesion Pathway Diagram Diseases associated with OPN elisa kit Disease Name Pubmed Publications Disease Models, Animal Antibodies >266 publications with OPN and Disease Models, Animal Inflammation Antibodies >261 publications with OPN and Inflammation Kidney Diseases Antibodies >233 publications with OPN and Kidney Diseases Fibrosis Antibodies >164 publications with OPN and Fibrosis Neoplasm Metastasis Antibodies >139 publications with OPN and Neoplasm Metastasis Necrosis Antibodies >132 publications with OPN and Necrosis Calcinosis Antibodies >116 publications with OPN and Calcinosis Adenocarcinoma Antibodies >111 publications with OPN and Adenocarcinoma Heart Diseases Antibodies >107 publications with OPN and Heart Diseases Neoplasms, Experimental Antibodies >85 publications with OPN and Neoplasms, Experimental Organs/Tissues associated with OPN elisa kit Organ/Tissue Name Pubmed Publications Bone Antibodies >1104 publications with OPN and Bone Blood Antibodies >561 publications with OPN and Blood Kidney Antibodies >369 publications with OPN and Kidney Vascular Antibodies >356 publications with OPN and Vascular Connective Tissue Antibodies >341 publications with OPN and Connective Tissue Muscle Antibodies >290 publications with OPN and Muscle Bone Marrow Antibodies >197 publications with OPN and Bone Marrow Mouth Antibodies >186 publications with OPN and Mouth Liver Antibodies >137 publications with OPN and Liver Heart Antibodies >135 publications with OPN and Heart TOP © 2006-24 MyBioSource.com. 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