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PDGF-D elisa kit :: Rat PDGF-D ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2504978
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PDGF-D, ELISA Kit

★Popular Item★
 Also Known As   

Rat PDGF-D (Platelet Derived Growth Factor D) ELISA Kit

 Product Gene Name   

PDGF-D elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    609673
 3D Structure    ModBase 3D Structure for Q9GZP0
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Rat PDGF-D. No significant cross-reactivity or interference between Rat PDGF-D and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rat PDGF-D and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    0.313--20ng/mL
 Sensitivity    0.188ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PDGF-D elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PDGF-D purchase    MBS2504978 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PDGF-D, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.313--20ng/mL in biological research samples containing PDGF-D, with an estimated sensitivity of 0.188ng/mL. The ELISA analytical biochemical technique of the MBS2504978 kit is based on PDGF-D antibody-PDGF-D antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PDGF-D antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PDGF-D. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PDGF-D elisa kit

   Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PDGF-D in Rat serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PDGF-D. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat PDGF-D and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PDGF-D, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat PDGF-D. You can calculate the concentration of Rat PDGF-D in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of PDGF-D elisa kit    PDGF-D elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2504978. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PDGF-D. It may not necessarily be applicable to this product.
 NCBI GI #    15451921
 NCBI GeneID    80310
 NCBI Accession #    NP_149126.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_033135.3 [Other Products]
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 UniProt Primary Accession #    Q9GZP0 [Other Products]
 UniProt Secondary Accession #    Q9BWV5; A8K9T6 [Other Products]
 UniProt Related Accession #    Q9GZP0 [Other Products]
 Molecular Weight    42,167 Da
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 NCBI Official Full Name    platelet-derived growth factor D isoform 2
 NCBI Official Synonym Full Names    platelet derived growth factor D
 NCBI Official Symbol    PDGFD [Similar Products]
 NCBI Official Synonym Symbols   
IEGF; SCDGFB; MSTP036; SCDGF-B
[Similar Products]
 NCBI Protein Information    platelet-derived growth factor D; PDGF-D; iris-expressed growth factor; spinal cord derived growth factor B; spinal cord-derived growth factor B; spinal cord-derived growth factor-B
 UniProt Protein Name    Platelet-derived growth factor D
 UniProt Synonym Protein Names   
Iris-expressed growth factor; Spinal cord-derived growth factor B; SCDGF-B
 UniProt Gene Name    PDGFD [Similar Products]
 UniProt Synonym Gene Names    IEGF; SCDGFB; PDGF-D; SCDGF-B; PDGFD latent form; PDGFD receptor-binding form [Similar Products]
 UniProt Entry Name    PDGFD_HUMAN
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 NCBI Summary for PDGF-D    The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. Two splice variants have been identified for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for PDGF-D    PDGFD: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Plays an important role in wound healing. Induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. Can initiate events that lead to a mesangial proliferative glomerulonephritis, including influx of monocytes and macrophages and production of extracellular matrix. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: Golgi membrane; endoplasmic reticulum lumen; extracellular region

Molecular Function: growth factor activity; platelet-derived growth factor receptor binding

Biological Process: positive regulation of cell division; multicellular organismal development; platelet-derived growth factor receptor signaling pathway; regulation of peptidyl-tyrosine phosphorylation
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 Research Articles on PDGF-D    1. Compared with matched normal endometrial cases, PDGF-D was up-regulated in endometrial cancer. Expression of PDGF-D protein, found in 78% of the cases, was associated with nonendometrioid histologic type.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PDGF-D elisa kitPathways associated with PDGF-D elisa kit
 Reference Product  PubMed Publications
 PDGFRB elisa kit  >36 publications with PDGF-D and PDGFRB
 PDGFRA elisa kit  >24 publications with PDGF-D and PDGFRA
 VEGFA elisa kit  >3 publications with PDGF-D and VEGFA
 FLT1 elisa kit  >3 publications with PDGF-D and FLT1
 FGFR1 elisa kit  >1 publications with PDGF-D and FGFR1
 EGFR elisa kit  >1 publications with PDGF-D and EGFR
 Products by Pathway  Pathway Diagram
 Choline Metabolism In Cancer Pathway antibodies  Choline Metabolism In Cancer Pathway Diagram
 Choline Metabolism In Cancer Pathway antibodies  Choline Metabolism In Cancer Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Gap Junction Pathway antibodies  Gap Junction Pathway Diagram
 Gap Junction Pathway antibodies  Gap Junction Pathway Diagram
 Melanoma Pathway antibodies  Melanoma Pathway Diagram
 Melanoma Pathway antibodies  Melanoma Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram
Diseases associated with PDGF-D elisa kitOrgans/Tissues associated with PDGF-D elisa kit
 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >11 publications with PDGF-D and Fibrosis
 Kidney Diseases Antibodies  >10 publications with PDGF-D and Kidney Diseases
 Nervous System Diseases Antibodies  >10 publications with PDGF-D and Nervous System Diseases
 Brain Diseases Antibodies  >8 publications with PDGF-D and Brain Diseases
 Cell Transformation, Neoplastic Antibodies  >6 publications with PDGF-D and Cell Transformation, Neoplastic
 Disease Models, Animal Antibodies  >6 publications with PDGF-D and Disease Models, Animal
 Inflammation Antibodies  >5 publications with PDGF-D and Inflammation
 Liver Diseases Antibodies  >4 publications with PDGF-D and Liver Diseases
 Necrosis Antibodies  >4 publications with PDGF-D and Necrosis
 Musculoskeletal Diseases Antibodies  >4 publications with PDGF-D and Musculoskeletal Diseases
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >25 publications with PDGF-D and Vascular
 Kidney Antibodies  >13 publications with PDGF-D and Kidney
 Prostate Antibodies  >12 publications with PDGF-D and Prostate
 Heart Antibodies  >8 publications with PDGF-D and Heart
 Brain Antibodies  >8 publications with PDGF-D and Brain
 Ovary Antibodies  >5 publications with PDGF-D and Ovary
 Connective Tissue Antibodies  >4 publications with PDGF-D and Connective Tissue
 Lymph Node Antibodies  >4 publications with PDGF-D and Lymph Node
 Lung Antibodies  >4 publications with PDGF-D and Lung
 Bone Antibodies  >4 publications with PDGF-D and Bone
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