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PKIN1 elisa kit :: Rat PKN1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2512414
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PKN1 (PKIN1), ELISA Kit

★Popular Item★
 Also Known As   

Rat PKN1 (Protein Kinase N1) ELISA Kit

 Product Gene Name   

PKIN1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    601032
 3D Structure    ModBase 3D Structure for Q16512
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 Species Reactivity    Rat
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 Specificity    This kit recognizes natural and recombinant Rat PKIN1. No significant cross-reactivity or interference between Rat PKIN1 and analogues was observed.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    1.563-100ng/mL
 Sensitivity    Min: 0.938ng/mL; Max: 100ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PKIN1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PKIN1 purchase    MBS2512414 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PKN1 (PKIN1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.563-100ng/mL in biological research samples containing PKIN1, with an estimated sensitivity of Min: 0.938ng/mL; Max: 100ng/mL. The ELISA analytical biochemical technique of the MBS2512414 kit is based on PKIN1 antibody-PKIN1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PKIN1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PKIN1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PKIN1 elisa kit

   Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat PKIN1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PKIN1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PKIN1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PKIN1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PKIN1. You can calculate the concentration of PKIN1 in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of PKIN1 elisa kit    PKIN1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2512414. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PKIN1. It may not necessarily be applicable to this product.
 NCBI GI #    63100313
 NCBI GeneID    5585
 NCBI Accession #    AAH94766.1 [Other Products]
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 UniProt Primary Accession #    Q16512 [Other Products]
 UniProt Secondary Accession #    Q15143; Q504U4; Q8IUV5; Q9UD44; A8K7W5; B2R9R4; B3KVN3 [Other Products]
 UniProt Related Accession #    Q16512 [Other Products]
 Molecular Weight    66,060 Da
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 NCBI Official Full Name    PKN1 protein
 NCBI Official Synonym Full Names    protein kinase N1
 NCBI Official Symbol    PKN1 [Similar Products]
 NCBI Official Synonym Symbols   
DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA
[Similar Products]
 NCBI Protein Information    serine/threonine-protein kinase N1; protease-activated kinase 1; protein kinase C-like 1; protein kinase C-like PKN; protein kinase C-related kinase 1; protein kinase PKN-alpha; protein-kinase C-related kinase 1; serine-threonine kinase N; serine-threonine protein kinase N; serine/threonine protein kinase N
 UniProt Protein Name    Serine/threonine-protein kinase N1
 UniProt Synonym Protein Names   
Protease-activated kinase 1; PAK-1; Protein kinase C-like 1; Protein kinase C-like PKN; Protein kinase PKN-alpha; Protein-kinase C-related kinase 1; Serine-threonine protein kinase N
 UniProt Gene Name    PKN1 [Similar Products]
 UniProt Synonym Gene Names    PAK1; PKN; PRK1; PRKCL1; PAK-1 [Similar Products]
 UniProt Entry Name    PKN1_HUMAN
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 NCBI Summary for PKIN1    The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
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 UniProt Comments for PKIN1    PKN1: an AGC kinase of the PKN family. A PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcriptional regulation. Regulates the cytoskeletal network by phosphorylating intermediate filament proteins such as VIM and NFH, NFL and NFM, inhibiting their polymerization. Phosphorylates Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of H3T11, a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3K9me by JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, impairing their import in the nucleus. Activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids. Activated by CASP3 cleavage during apoptosis. Localization to endosomes is mediated via its interaction with RHOB. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis. Three isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, AGC; EC 2.7.11.13; Protein kinase, Ser/Thr (non-receptor); AGC group; PKN family

Chromosomal Location of Human Ortholog: 19p13.12

Cellular Component: cytoplasmic membrane-bound vesicle; cytoplasm; plasma membrane; midbody; nucleus; endosome; cleavage furrow

Molecular Function: protein serine/threonine kinase activity; protein binding; protein kinase C activity; ligand-dependent nuclear receptor transcription coactivator activity; androgen receptor binding; protein kinase C binding; histone binding; histone deacetylase binding; Rac GTPase binding; GTP-Rho binding; chromatin binding; ATP binding; protein kinase activity

Biological Process: regulation of transcription from RNA polymerase II promoter; hyperosmotic response; transcription, DNA-dependent; signal transduction; protein amino acid phosphorylation; activation of JNK activity
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 Research Articles on PKIN1    1. Data indicate that Salmonella SspH1 catalyzes the ubiquitination and proteasome-dependent degradation of PKN1 in cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PKIN1 elisa kitPathways associated with PKIN1 elisa kit
 Reference Product  PubMed Publications
 RHOA elisa kit  >3 publications with PKIN1 and RHOA
 PDPK1 elisa kit  >1 publications with PKIN1 and PDPK1
 Products by Pathway  Pathway Diagram
 G13 Signaling Pathway antibodies  G13 Signaling Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram
 Regulation Of Androgen Receptor Activity Pathway antibodies  Regulation Of Androgen Receptor Activity Pathway Diagram
 RhoA Signaling Pathway antibodies  RhoA Signaling Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Signaling Mediated By P38-gamma And P38-delta Pathway antibodies  Signaling Mediated By P38-gamma And P38-delta Pathway Diagram
 TNF-alpha/NF-kB Signaling Pathway antibodies  TNF-alpha/NF-kB Signaling Pathway Diagram
Diseases associated with PKIN1 elisa kitOrgans/Tissues associated with PKIN1 elisa kit
 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >3 publications with PKIN1 and Immune System Diseases
 Necrosis Antibodies  >3 publications with PKIN1 and Necrosis
 Breast Neoplasms Antibodies  >2 publications with PKIN1 and Breast Neoplasms
 Nephritis Antibodies  >1 publications with PKIN1 and Nephritis
 Pneumonia Antibodies  >1 publications with PKIN1 and Pneumonia
 Disease Models, Animal Antibodies  >1 publications with PKIN1 and Disease Models, Animal
 Urinary Bladder Neoplasms Antibodies  >1 publications with PKIN1 and Urinary Bladder Neoplasms
 Kidney Diseases Antibodies  >1 publications with PKIN1 and Kidney Diseases
 Inflammation Antibodies  >1 publications with PKIN1 and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Prostate Antibodies  >4 publications with PKIN1 and Prostate
 Muscle Antibodies  >3 publications with PKIN1 and Muscle
 Blood Antibodies  >3 publications with PKIN1 and Blood
 Lymph Node Antibodies  >2 publications with PKIN1 and Lymph Node
 Brain Antibodies  >2 publications with PKIN1 and Brain
 Spleen Antibodies  >1 publications with PKIN1 and Spleen
 Bone Antibodies  >1 publications with PKIN1 and Bone
 Mammary Gland Antibodies  >1 publications with PKIN1 and Mammary Gland
 Uterus Antibodies  >1 publications with PKIN1 and Uterus
 Nerve Antibodies  >1 publications with PKIN1 and Nerve
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