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PRF elisa kit :: Rat Perforin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS034161 PRF elisa kit
Unit / Price
48-Strip-Wells  /  $435 +1 FREE 8GB USB
96-Strip-Wells  /  $600 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,650 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,205 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Perforin (PRF), ELISA Kit

 Also Known As   

Rat Perforin ELISA Kit

 Product Gene Name   

PRF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 Chromosome Location    Chromosome: 10; NC_000010.10 (72357104..72362531, complement). Location: 10q22
 OMIM    170280
 3D Structure    ModBase 3D Structure for P14222
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 Species Reactivity    Rat
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 Specificity    No significant cross-reactivity or interference between this analyte and analogues is observed.
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 Samples    Undiluted original Rat body fluids, tissue homogenates, secretions or feces samples.
 Assay Type    Sandwich (Quantitative)
 Detection Range    31.2 pg/ml - 1000 pg/ml.
 Sensitivity    5.0 pg/ml.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100].
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of PRF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PRF purchase    MBS034161 is a ready-to-use microwell, strip plate Sandwich (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Perforin (PRF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.2 pg/ml - 1000 pg/ml in biological research samples containing PRF, with an estimated sensitivity of 5.0 pg/ml. The ELISA analytical biochemical technique of the MBS034161 kit is based on PRF antibody-PRF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PRF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PRF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Undiluted original Rat body fluids, tissue homogenates, secretions or feces samples. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for PRF elisa kit

   Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of PRF (hereafter termed "analyte") in undiluted original Rat body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
Sample Manual Insert of MBS034161
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NCBI/Uniprot data below describe general gene information for PRF. It may not necessarily be applicable to this product.
 NCBI GI #    189847
 NCBI GeneID    5551
 NCBI Accession #    AAA60065.1 [Other Products]
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 UniProt Primary Accession #    P14222 [Other Products]
 UniProt Secondary Accession #    Q59F57; Q86WX7; B2R6X4 [Other Products]
 UniProt Related Accession #    P14222 [Other Products]
 Molecular Weight    61,377 Da
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 NCBI Official Full Name    perforin
 NCBI Official Synonym Full Names    perforin 1 (pore forming protein)
 NCBI Official Symbol    PRF1 [Similar Products]
 NCBI Official Synonym Symbols   
P1; PFP; FLH2; PFN1; HPLH2
[Similar Products]
 NCBI Protein Information    perforin-1; cytolysin; lymphocyte pore forming protein; lymphocyte pore-forming protein
 UniProt Protein Name    Perforin-1
 UniProt Synonym Protein Names   
Cytolysin; Lymphocyte pore-forming protein
 Protein Family    Perforin
 UniProt Gene Name    PRF1 [Similar Products]
 UniProt Synonym Gene Names    PFP; P1; PFP [Similar Products]
 UniProt Entry Name    PERF_HUMAN
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 NCBI Summary for PRF    The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
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 UniProt Comments for PRF    PRF1: Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. Monomer, as sobluble protein. Homooligomer. Oligomerization is required for pore formation. Repressed by contact with target cells. Belongs to the complement C6/C7/C8/C9 family.

Protein type: Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 10q22

Cellular Component: membrane; cytoplasmic membrane-bound vesicle; plasma membrane; integral to membrane; extracellular region

Molecular Function: protein binding; calcium ion binding; wide pore channel activity

Biological Process: formation of immunological synapse; apoptosis; cytolysis; defense response to tumor cell; cellular defense response; immune response to tumor cell; transmembrane transport; defense response to virus; protein homooligomerization

Disease: Lymphoma, Non-hodgkin, Familial; Aplastic Anemia; Hemophagocytic Lymphohistiocytosis, Familial, 2
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 Research Articles on PRF    1. The mRNA and protein expression of perforin significantly increases in CD4(+) and CD8(+) T cells treated with 5-azaC, which is associated with DNA hypomethylation of perforin promoter in T cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PRF elisa kitPathways associated with PRF elisa kit
 Reference Product  PubMed Publications
 GZMB elisa kit  >526 publications with PRF and GZMB
 IL2RA elisa kit  >42 publications with PRF and IL2RA
 CD86 elisa kit  >38 publications with PRF and CD86
 EOMES elisa kit  >19 publications with PRF and EOMES
 IL2 elisa kit  >13 publications with PRF and IL2
 IL2RB elisa kit  >12 publications with PRF and IL2RB
 CD3D elisa kit  >3 publications with PRF and CD3D
 CD3E elisa kit  >1 publications with PRF and CD3E
 CD247 elisa kit  >1 publications with PRF and CD247
 Products by Pathway  Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
 Downstream Signaling In Naive CD8+ T Cells Pathway antibodies  Downstream Signaling In Naive CD8+ T Cells Pathway Diagram
 Graft-versus-host Disease Pathway antibodies  Graft-versus-host Disease Pathway Diagram
 Graft-versus-host Disease Pathway antibodies  Graft-versus-host Disease Pathway Diagram
 IL12 Signaling Mediated By STAT4 Pathway antibodies  IL12 Signaling Mediated By STAT4 Pathway Diagram
Diseases associated with PRF elisa kitOrgans/Tissues associated with PRF elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >961 publications with PRF and Neoplasms
 Immune System Diseases Antibodies  >893 publications with PRF and Immune System Diseases
 Necrosis Antibodies  >655 publications with PRF and Necrosis
 Neoplasms, Experimental Antibodies  >273 publications with PRF and Neoplasms, Experimental
 Inflammation Antibodies  >267 publications with PRF and Inflammation
 Carcinoma Antibodies  >193 publications with PRF and Carcinoma
 Liver Diseases Antibodies  >172 publications with PRF and Liver Diseases
 Lymphoma, Non-Hodgkin Antibodies  >152 publications with PRF and Lymphoma, Non-Hodgkin
 Cardiovascular Diseases Antibodies  >139 publications with PRF and Cardiovascular Diseases
 Drug Toxicity Antibodies  >89 publications with PRF and Drug Toxicity
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >3686 publications with PRF and Blood
 Liver Antibodies  >305 publications with PRF and Liver
 Spleen Antibodies  >297 publications with PRF and Spleen
 Bone Marrow Antibodies  >249 publications with PRF and Bone Marrow
 Lung Antibodies  >218 publications with PRF and Lung
 Kidney Antibodies  >183 publications with PRF and Kidney
 Lymph Node Antibodies  >175 publications with PRF and Lymph Node
 Brain Antibodies  >140 publications with PRF and Brain
 Muscle Antibodies  >123 publications with PRF and Muscle
 Intestine Antibodies  >97 publications with PRF and Intestine
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