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PPAR-gamma elisa kit :: Peroxisome Proliferators activator gamma ELISA Kit

Scan QR to view Datasheet Catalog #    MBS725973
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Peroxisome Proliferators activator gamma (PPAR-gamma), ELISA Kit

★Popular Item★
 Also Known As   

Rat Peroxisome Proliferators activator gamma ELISA Kit

 Product Synonym Names    Rat Peroxisome Proliferators activator g ELISA Kit
 Product Gene Name   

PPAR-gamma elisa kit

[Similar Products]
 Product Synonym Gene Name    PPAR-g [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    609338
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of PPAgamma. No significant cross-reactivity or interference between PPAgamma and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PPAgamma and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    0.5-10ng/mL
 Sensitivity    0.1ng/ml
 Intended Uses    This PPAgamma ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat PPAgamma. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PPAR-gamma elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PPAR-gamma purchase    MBS725973 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Peroxisome Proliferators activator gamma (PPAR-gamma) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.5-10ng/mL in biological research samples containing PPAR-gamma, with an estimated sensitivity of 0.1ng/ml. The ELISA analytical biochemical technique of the MBS725973 kit is based on PPAR-gamma antibody-PPAR-gamma antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PPAR-gamma antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PPAR-gamma. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PPAR-gamma elisa kit

   Principle of the assay: PPAgamma ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PPAgamma antibody and an PPAgamma-HRP conjugate. The assay sample and buffer are incubated together with PPAgamma-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PPAgamma concentration since PPAgamma from samples and PPAgamma-HRP conjugate compete for the anti-PPAgamma antibody binding site. Since the number of sites is limited, as more sites are occupied by PPAgamma from the sample, fewer sites are left to bind PPAgamma-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PPAgamma concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of PPAR-gamma elisa kit    PPAR-gamma elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS725973. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PPAR-gamma. It may not necessarily be applicable to this product.
 NCBI GI #    13432234
 NCBI GeneID    5468
 NCBI Accession #    P37231.3 [Other Products]
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 UniProt Secondary Accession #    O00684; O00710; O14515; Q0QJH8; Q15178; Q15179; Q15180; Q15832; Q86U60; A8K3G6; B5BUA1 [Other Products]
 UniProt Related Accession #    P37231 [Other Products]
 Molecular Weight    21,580 Da
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 NCBI Official Full Name    Peroxisome proliferator-activated receptor gamma
 NCBI Official Synonym Full Names    peroxisome proliferator-activated receptor gamma
 NCBI Official Symbol    PPARG [Similar Products]
 NCBI Official Synonym Symbols   
GLM1; CIMT1; NR1C3; PPARG1; PPARG2; PPARgamma
[Similar Products]
 NCBI Protein Information    peroxisome proliferator-activated receptor gamma; PPAR-gamma; nuclear receptor subfamily 1 group C member 3; peroxisome proliferator-activated nuclear receptor gamma variant 1
 UniProt Protein Name    Peroxisome proliferator-activated receptor gamma
 UniProt Synonym Protein Names   
Nuclear receptor subfamily 1 group C member 3
 UniProt Gene Name    PPARG [Similar Products]
 UniProt Synonym Gene Names    NR1C3; PPAR-gamma [Similar Products]
 UniProt Entry Name    PPARG_HUMAN
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 NCBI Summary for PPAR-gamma    This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008]
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 UniProt Comments for PPAR-gamma    PPAR-gamma: a transcription factor, member of the nuclear hormone receptor superfamily. Receptor for hypolipidemic drugs and fatty acids. Preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage. Regulator of adipogenesis and lipid metabolism, modulates insulin sensitivity, cell proliferation and inflammation. Phosphorylated and inhibited by MAP kinase. Heterodimerizes with the retinoid X receptor. Interacts with NCOA6 coactivator, leading to a strong increase in transcription of target genes. Two splice-variant isoforms have been described.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 3p25

Cellular Component: nucleoplasm; perinuclear region of cytoplasm; cytosol; nucleus

Molecular Function: ligand-dependent nuclear receptor activity; transcription activator binding; zinc ion binding; drug binding; alpha-actinin binding; protein phosphatase binding; retinoid X receptor binding; arachidonic acid binding; protein binding; enzyme binding; ligand-dependent nuclear receptor transcription coactivator activity; DNA binding; prostaglandin receptor activity; sequence-specific DNA binding; estrogen receptor binding; steroid hormone receptor activity; chromatin binding; transcription factor activity

Biological Process: positive regulation of transcription, DNA-dependent; heart development; negative regulation of collagen biosynthetic process; cell maturation; rhythmic process; lipid homeostasis; response to lipid; glucose homeostasis; response to caffeine; response to vitamin A; positive regulation of oligodendrocyte differentiation; placenta development; long-chain fatty acid transport; organ regeneration; cell fate commitment; monocyte differentiation; negative regulation of acute inflammatory response; regulation of circadian rhythm; response to starvation; negative regulation of telomerase activity; cellular response to insulin stimulus; response to mechanical stimulus; response to estrogen stimulus; lipoprotein transport; brown fat cell differentiation; positive regulation of fat cell differentiation; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; fatty acid oxidation; positive regulation of transcription factor activity; negative regulation of transcription, DNA-dependent; positive regulation of phagocytosis, engulfment; negative regulation of smooth muscle cell proliferation; low-density lipoprotein receptor biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; signal transduction; epithelial cell differentiation; regulation of blood pressure; response to nutrient; caspase activation; transcription initiation from RNA polymerase II promoter; response to retinoic acid; G-protein coupled receptor protein signaling pathway; response to low density lipoprotein stimulus; white fat cell differentiation; positive regulation of fatty acid oxidation; innate immune response; gene expression; response to cold; negative regulation of cell growth; lipid metabolic process

Disease: Obesity; Carotid Intimal Medial Thickness 1; Lipodystrophy, Familial Partial, Type 3; Diabetes Mellitus, Noninsulin-dependent
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 Research Articles on PPAR-gamma    1. LOX, LOXL1 and LOXL2 are downstream PPARgamma targets and that LOX activity is a negative regulator of trophoblastic cell invasion
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with PPAR-gamma elisa kitDiseases associated with PPAR-gamma elisa kit
 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Energy Metabolism Pathway antibodies  Energy Metabolism Pathway Diagram
 Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies  Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Disease Name  Pubmed Publications
 Metabolic Diseases Antibodies  >2646 publications with PPAR-gamma and Metabolic Diseases
 Inflammation Antibodies  >1824 publications with PPAR-gamma and Inflammation
 Obesity Antibodies  >1809 publications with PPAR-gamma and Obesity
 Insulin Resistance Antibodies  >1683 publications with PPAR-gamma and Insulin Resistance
 Diabetes Mellitus Antibodies  >1676 publications with PPAR-gamma and Diabetes Mellitus
 Diabetes Mellitus, Type 2 Antibodies  >1133 publications with PPAR-gamma and Diabetes Mellitus, Type 2
 Necrosis Antibodies  >936 publications with PPAR-gamma and Necrosis
 Fatty Liver Antibodies  >762 publications with PPAR-gamma and Fatty Liver
 Atherosclerosis Antibodies  >716 publications with PPAR-gamma and Atherosclerosis
 Hypertension Antibodies  >569 publications with PPAR-gamma and Hypertension
Organs/Tissues associated with PPAR-gamma elisa kit
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >1775 publications with PPAR-gamma and Liver
 Adipose Tissue Antibodies  >1768 publications with PPAR-gamma and Adipose Tissue
 Connective Tissue Antibodies  >1285 publications with PPAR-gamma and Connective Tissue
 Vascular Antibodies  >1193 publications with PPAR-gamma and Vascular
 Heart Antibodies  >731 publications with PPAR-gamma and Heart
 Bone Antibodies  >642 publications with PPAR-gamma and Bone
 Brain Antibodies  >588 publications with PPAR-gamma and Brain
 Kidney Antibodies  >525 publications with PPAR-gamma and Kidney
 Lung Antibodies  >451 publications with PPAR-gamma and Lung
 Intestine Antibodies  >252 publications with PPAR-gamma and Intestine
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