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PI3K elisa kit :: Rat Phosphotylinosital 3 kinase (PI3K) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS260381
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $275 +1 FREE 8GB USB
96-Strip-Wells  /  $435 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Phosphotylinosital 3 kinase (PI3K), ELISA Kit

★Popular Item★
 Also Known As   

Rat Phosphotylinosital 3 kinase (PI3K) ELISA Kit

 Product Gene Name   

PI3K elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    602925
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 Species Reactivity    Rat
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 Specificity    No cross-reaction with other factors.
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 Samples    Serum, plasma or cell culture supernatant and organizations in the natural and recombinant PI3K concentration.
 Assay Type    Sandwich
 Detection Range    20 ng/ml-0.312 ng/ml
 Sensitivity    0.06 ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PI3K elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PI3K purchase    MBS260381 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phosphotylinosital 3 kinase (PI3K) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 20 ng/ml-0.312 ng/ml in biological research samples containing PI3K, with an estimated sensitivity of 0.06 ng/ml. The ELISA analytical biochemical technique of the MBS260381 kit is based on PI3K antibody-PI3K antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PI3K antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PI3K. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma or cell culture supernatant and organizations in the natural and recombinant PI3K concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PI3K elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat PI3K monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of PI3K elisa kit    PI3K elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS260381. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PI3K. It may not necessarily be applicable to this product.
 NCBI GI #    5453894
 NCBI GeneID    5291
 NCBI Accession #    NP_006210.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_006219.2 [Other Products]
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 UniProt Secondary Accession #    Q24JU2; D3DNF0 [Other Products]
 UniProt Related Accession #    P42338 [Other Products]
 Molecular Weight    122,762 Da
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 NCBI Official Full Name    phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform isoform 1
 NCBI Official Synonym Full Names    phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit beta
 NCBI Official Symbol    PIK3CB [Similar Products]
 NCBI Official Synonym Symbols   
PI3K; PIK3C1; P110BETA; PI3KBETA
[Similar Products]
 NCBI Protein Information    phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform; PI3K-beta; PtdIns-3-kinase p110; PI3-kinase subunit beta; PI3-kinase p110 subunit beta; ptdIns-3-kinase subunit beta; ptdIns-3-kinase subunit p110-beta; phosphoinositide-3-kinase, catalytic, beta polypeptide; phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta; phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta
 UniProt Protein Name    Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform
 UniProt Synonym Protein Names   
Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta; PtdIns-3-kinase subunit p110-beta; p110beta
 UniProt Gene Name    PIK3CB [Similar Products]
 UniProt Synonym Gene Names    PIK3C1; PI3-kinase subunit beta; PI3K-beta; PI3Kbeta; PtdIns-3-kinase subunit beta; PtdIns-3-kinase subunit p110-beta; p110beta [Similar Products]
 UniProt Entry Name    PK3CB_HUMAN
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 NCBI Summary for PI3K    This gene encodes an isoform of the catalytic subunit of phosphoinositide 3-kinase (PI3K). These kinases are important in signaling pathways involving receptors on the outer membrane of eukaryotic cells and are named for their catalytic subunit. The encoded protein is the catalytic subunit for PI3Kbeta (PI3KB). PI3KB has been shown to be part of the activation pathway in neutrophils which have bound immune complexes at sites of injury or infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]
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 UniProt Comments for PI3K    PIK3CB: catalytic subunit of phosphoinositide-3-kinase beta. Catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP) and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration and cell survival. PTEN reverses this process, and the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN. Expressed ubiquitously.

Protein type: EC 2.7.1.153; Kinase, lipid; Motility/polarity/chemotaxis; Carbohydrate Metabolism - inositol phosphate

Chromosomal Location of Human Ortholog: 3q22.3

Cellular Component: plasma membrane; nucleus; phosphoinositide 3-kinase complex; cytosol

Molecular Function: protein binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; 1-phosphatidylinositol-3-kinase activity; insulin receptor substrate binding; phosphatidylinositol-4-phosphate 3-kinase activity; ATP binding; phosphoinositide 3-kinase activity

Biological Process: phosphoinositide 3-kinase cascade; nerve growth factor receptor signaling pathway; regulation of cell-matrix adhesion; activation of MAPK activity; signal transduction; chemotaxis; T cell receptor signaling pathway; embryonic cleavage; phosphoinositide phosphorylation; phospholipid metabolic process; positive regulation of autophagy; epidermal growth factor receptor signaling pathway; platelet activation; cell migration; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; insulin receptor signaling pathway; phosphatidylinositol biosynthetic process; innate immune response; autophagy; vascular endothelial growth factor receptor signaling pathway; homophilic cell adhesion; blood coagulation; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway
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 Research Articles on PI3K    1. results suggested that down-regulation of p110beta expression by siRNA obviously reduced cell number via accumulation in G(0)-G(1) phase of the cell cycle.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PI3K elisa kitPathways associated with PI3K elisa kit
 Reference Product  PubMed Publications
 SRC elisa kit  >48 publications with PI3K and SRC
 PTEN elisa kit  >48 publications with PI3K and PTEN
 PIK3R1 elisa kit  >28 publications with PI3K and PIK3R1
 AKT1 elisa kit  >18 publications with PI3K and AKT1
 IRS1 elisa kit  >17 publications with PI3K and IRS1
 PIK3R2 elisa kit  >7 publications with PI3K and PIK3R2
 GAB2 elisa kit  >5 publications with PI3K and GAB2
 SYK elisa kit  >5 publications with PI3K and SYK
 LYN elisa kit  >4 publications with PI3K and LYN
 Products by Pathway  Pathway Diagram
 3-phosphoinositide Biosynthesis Pathway antibodies  3-phosphoinositide Biosynthesis Pathway Diagram
 3-phosphoinositide Biosynthesis Pathway antibodies  3-phosphoinositide Biosynthesis Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
Diseases associated with PI3K elisa kitOrgans/Tissues associated with PI3K elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >32 publications with PI3K and Inflammation
 Breast Neoplasms Antibodies  >29 publications with PI3K and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >28 publications with PI3K and Cell Transformation, Neoplastic
 Disease Models, Animal Antibodies  >28 publications with PI3K and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >17 publications with PI3K and Neoplasms, Experimental
 Necrosis Antibodies  >14 publications with PI3K and Necrosis
 Hypertrophy Antibodies  >9 publications with PI3K and Hypertrophy
 Ovarian Neoplasms Antibodies  >9 publications with PI3K and Ovarian Neoplasms
 Neoplasm Invasiveness Antibodies  >9 publications with PI3K and Neoplasm Invasiveness
 Lung Neoplasms Antibodies  >8 publications with PI3K and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >127 publications with PI3K and Blood
 Muscle Antibodies  >41 publications with PI3K and Muscle
 Vascular Antibodies  >33 publications with PI3K and Vascular
 Bone Antibodies  >26 publications with PI3K and Bone
 Lung Antibodies  >23 publications with PI3K and Lung
 Bone Marrow Antibodies  >20 publications with PI3K and Bone Marrow
 Liver Antibodies  >19 publications with PI3K and Liver
 Brain Antibodies  >15 publications with PI3K and Brain
 Prostate Antibodies  >11 publications with PI3K and Prostate
 Skin Antibodies  >9 publications with PI3K and Skin
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