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PECAM1 elisa kit :: Rat Platelet/Endothelial Cell Adhesion Molecule (PECAM1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2024246 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $265 +1 FREE 8GB USB
48-Strip-Wells  /  $490 +1 FREE 8GB USB
96-Strip-Wells  /  $655 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,660 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,795 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Platelet/Endothelial Cell Adhesion Molecule (PECAM1), ELISA Kit

★Popular Item★
 Also Known As   

Platelet/Endothelial Cell Adhesion Molecule (PECAM1) ELISA Kit

 Product Synonym Names    CD31; EndoCAM; GPIIA'; PECA1
 Product Gene Name   

PECAM1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    173445
 3D Structure    ModBase 3D Structure for P16284
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Platelet/Endothelial Cell Adhesion Molecule (PECAM1).
No significant cross-reactivity or interference between Platelet/Endothelial Cell Adhesion Molecule (PECAM1) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue homogenates and Other Biological Fluids
 Detection Range    31.2-2000pg/mL
 Sensitivity    < 13.2pg/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Platelet/Endothelial Cell Adhesion Molecule (PECAM1) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Platelet/Endothelial Cell Adhesion Molecule (PECAM1) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PECAM1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PECAM1 purchase    MBS2024246 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Platelet/Endothelial Cell Adhesion Molecule (PECAM1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.2-2000pg/mL in biological research samples containing PECAM1, with an estimated sensitivity of < 13.2pg/mL. The ELISA analytical biochemical technique of the MBS2024246 kit is based on PECAM1 antibody-PECAM1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PECAM1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PECAM1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue homogenates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PECAM1 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet/Endothelial Cell Adhesion Molecule (PECAM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet/Endothelial Cell Adhesion Molecule (PECAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet/Endothelial Cell Adhesion Molecule (PECAM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Platelet/Endothelial Cell Adhesion Molecule (PECAM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for PECAM1 elisa kit    CD & Adhesion molecule; Infection immunity
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 Typical Testing Data/Standard Curve (for reference only) of PECAM1 elisa kit    PECAM1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2024246. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PECAM1. It may not necessarily be applicable to this product.
 NCBI GI #    313760624
 NCBI GeneID    5175
 NCBI Accession #    NP_000433.4 [Other Products]
 NCBI GenBank Nucleotide #    NM_000442.4 [Other Products]
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 UniProt Primary Accession #    P16284 [Other Products]
 UniProt Secondary Accession #    Q6LDA9; Q8TBH1; Q96RF5; Q96RF6; Q9NP65; Q9NPB7; Q9NPG9; Q9NQS9; Q9NQT0; A8K3S7; D3DU31 [Other Products]
 UniProt Related Accession #    P16284 [Other Products]
 Molecular Weight    81,378 Da
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 NCBI Official Full Name    platelet endothelial cell adhesion molecule
 NCBI Official Synonym Full Names    platelet/endothelial cell adhesion molecule 1
 NCBI Official Symbol    PECAM1 [Similar Products]
 NCBI Official Synonym Symbols   
CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
[Similar Products]
 NCBI Protein Information    platelet endothelial cell adhesion molecule
 UniProt Protein Name    Platelet endothelial cell adhesion molecule
 UniProt Synonym Protein Names   
EndoCAM; GPIIA'; PECA1; CD_antigen: CD31
 Protein Family    Platelet endothelial cell adhesion molecule
 UniProt Gene Name    PECAM1 [Similar Products]
 UniProt Synonym Gene Names    PECAM-1 [Similar Products]
 UniProt Entry Name    PECA1_HUMAN
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 NCBI Summary for PECAM1    The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]
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 UniProt Comments for PECAM1    PECAM-1: a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Plays a role in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, cell migration, negative regulation of immune cell signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. Undergoes alternative splicing, generating multiple isoforms in vascular beds of various tissues.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: extracellular space; platelet alpha granule membrane; integral to membrane; plasma membrane; cell junction

Molecular Function: protein binding

Biological Process: platelet activation; extracellular matrix organization and biogenesis; platelet degranulation; diapedesis; cell recognition; blood coagulation; cell adhesion; signal transduction; leukocyte migration; phagocytosis
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 Research Articles on PECAM1    1. there are associations between various PECAM-1 polymorphisms in rheumatoid arthritis and systemic lupus erythematosus patients, and PECAM-1 polymorphisms in SLE are protective against atherosclerotic complications
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with PECAM1 elisa kitDiseases associated with PECAM1 elisa kit
 Products by Pathway  Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Integrin Cell Surface Interactions Pathway antibodies  Integrin Cell Surface Interactions Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Malaria Pathway antibodies  Malaria Pathway Diagram
 Malaria Pathway antibodies  Malaria Pathway Diagram
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >630 publications with PECAM1 and Cardiovascular Diseases
 Inflammation Antibodies  >502 publications with PECAM1 and Inflammation
 Necrosis Antibodies  >404 publications with PECAM1 and Necrosis
 Neoplasms, Experimental Antibodies  >264 publications with PECAM1 and Neoplasms, Experimental
 Lung Diseases Antibodies  >246 publications with PECAM1 and Lung Diseases
 Heart Diseases Antibodies  >231 publications with PECAM1 and Heart Diseases
 Breast Neoplasms Antibodies  >209 publications with PECAM1 and Breast Neoplasms
 Lung Neoplasms Antibodies  >193 publications with PECAM1 and Lung Neoplasms
 Liver Diseases Antibodies  >179 publications with PECAM1 and Liver Diseases
 Liver Neoplasms Antibodies  >156 publications with PECAM1 and Liver Neoplasms
Organs/Tissues associated with PECAM1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >3432 publications with PECAM1 and Vascular
 Blood Antibodies  >3024 publications with PECAM1 and Blood
 Muscle Antibodies  >690 publications with PECAM1 and Muscle
 Embryonic Tissue Antibodies  >511 publications with PECAM1 and Embryonic Tissue
 Lung Antibodies  >440 publications with PECAM1 and Lung
 Heart Antibodies  >355 publications with PECAM1 and Heart
 Skin Antibodies  >341 publications with PECAM1 and Skin
 Brain Antibodies  >325 publications with PECAM1 and Brain
 Connective Tissue Antibodies  >247 publications with PECAM1 and Connective Tissue
 Kidney Antibodies  >239 publications with PECAM1 and Kidney
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