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PF4 elisa kit :: Rat Platelet Factor 4 (PF4) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS451297 (SPECIAL PROMOTIONAL PRICING for a limited time)
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $355 +1 FREE 8GB USB
96-Strip-Wells  /  $485 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,160 +2 FREE 8GB USB
10x96-Strip-Wells  /  $4,185 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Platelet Factor 4 (PF4), ELISA Kit

★Popular Item★
 Also Known As   

Rat Platelet Factor 4 (PF4) ELISA Kit

 Product Synonym Names    CXCL4; SCYB4; Chemokine C-X-C-Motif Ligand 4; Oncostatin-A; Iroplact
 Product Gene Name   

PF4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P06765
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of PF4. No significant cross-reactivity or interference between PF4 and analogues was observed.
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Sandwich
 Detection Range    0.312-20ng/mL
 Sensitivity    0.128ng/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PF4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PF4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PF4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PF4 purchase    MBS451297 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Platelet Factor 4 (PF4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20ng/mL in biological research samples containing PF4, with an estimated sensitivity of 0.128ng/mL. The ELISA analytical biochemical technique of the MBS451297 kit is based on PF4 antibody-PF4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PF4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PF4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PF4 elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PF4 in rat serum, plasma and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PF4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PF4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Standard Curve/Testing Data of PF4 elisa kit    PF4 elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS451297. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PF4. It may not necessarily be applicable to this product.
 NCBI GI #    56090531
 NCBI GeneID    360918
 NCBI Accession #    NP_001007730.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001007729.1 [Other Products]
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 UniProt Primary Accession #    P06765 [Other Products]
 UniProt Related Accession #    P06765 [Other Products]
 Molecular Weight    11,287 Da
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 NCBI Official Full Name    platelet factor 4
 NCBI Official Synonym Full Names    platelet factor 4
 NCBI Official Symbol    Pf4 [Similar Products]
 NCBI Official Synonym Symbols   
PF-4; Pf4a; Cxcl4; RATPF4A
[Similar Products]
 NCBI Protein Information    platelet factor 4
 UniProt Protein Name    Platelet factor 4
 UniProt Synonym Protein Names   
C-X-C motif chemokine 4
 Protein Family    Platelet factor
 UniProt Gene Name    Pf4 [Similar Products]
 UniProt Synonym Gene Names    Cxcl4; Scyb4; PF-4 [Similar Products]
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 NCBI Summary for PF4    member of low-molecular weight chemokines superfamily; may mediate inflammation and immune response [RGD, Feb 2006]
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 UniProt Comments for PF4    Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation.
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 Research Articles on PF4    1. The levels of PF-4 (as a platelet marker) mRNA were significantly higher in FeCl(2)-VT (2(-DeltaDeltaCT)=7.8) than in stasis-VT (2(-DeltaDeltaCT)=4.2, p<0.05).
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PF4 elisa kitPathways associated with PF4 elisa kit
 Reference Product  PubMed Publications
 Ppbp elisa kit  >20 publications with PF4 and Ppbp
 Cxcr3 elisa kit  >14 publications with PF4 and Cxcr3
 Proc elisa kit  >13 publications with PF4 and Proc
 Vegfa elisa kit  >5 publications with PF4 and Vegfa
 Alb elisa kit  >3 publications with PF4 and Alb
 Cfd elisa kit  >2 publications with PF4 and Cfd
 Plg elisa kit  >2 publications with PF4 and Plg
 Products by Pathway  Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Common Pathway Of Fibrin Clot Formation antibodies  Common Pathway Of Fibrin Clot Formation Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
Diseases associated with PF4 elisa kitOrgans/Tissues associated with PF4 elisa kit
 Disease Name  Pubmed Publications
 Thrombocytopenia Antibodies  >548 publications with PF4 and Thrombocytopenia
 Cardiovascular Diseases Antibodies  >400 publications with PF4 and Cardiovascular Diseases
 Vascular Diseases Antibodies  >371 publications with PF4 and Vascular Diseases
 Thrombosis Antibodies  >335 publications with PF4 and Thrombosis
 Heart Diseases Antibodies  >125 publications with PF4 and Heart Diseases
 Inflammation Antibodies  >87 publications with PF4 and Inflammation
 Nervous System Diseases Antibodies  >87 publications with PF4 and Nervous System Diseases
 Autoimmune Diseases Antibodies  >66 publications with PF4 and Autoimmune Diseases
 Kidney Diseases Antibodies  >62 publications with PF4 and Kidney Diseases
 Atherosclerosis Antibodies  >59 publications with PF4 and Atherosclerosis
 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >119 publications with PF4 and Heart
 Kidney Antibodies  >70 publications with PF4 and Kidney
 Liver Antibodies  >45 publications with PF4 and Liver
 Lung Antibodies  >39 publications with PF4 and Lung
 Spleen Antibodies  >20 publications with PF4 and Spleen
 Embryonic Tissue Antibodies  >18 publications with PF4 and Embryonic Tissue
 Placenta Antibodies  >1 publications with PF4 and Placenta
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