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PAPPA elisa kit :: Rat Pregnancy Associated Plasma Protein A ELISA Kit

Scan QR to view Datasheet Catalog #    MBS762955
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $295 +1 FREE 8GB USB
96-Strip-Wells  /  $390 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,015 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Pregnancy Associated Plasma Protein A (PAPPA), ELISA Kit

 Also Known As   

Rat Pregnancy Associated Plasma Protein A ELISA Kit

 Product Synonym Names    PAPP-A (Pappalysin-1)/PAPPA/PAPA/DIPLA1/IGFBP-4ase/PAPPA1/ASBABP2/Pappalysin 1/Pregnancy Associated Plasma Protein A
 Product Gene Name   

PAPPA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS762955 COA    COA PDF
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of PAPPA. No significant cross-reactivity or interference between PAPPA and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    0.156-10ng/ml
 Sensitivity    <0.094ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PAPPA were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PAPPA were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PAPPA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PAPPA purchase    MBS762955 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Pregnancy Associated Plasma Protein A (PAPPA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/ml in biological research samples containing PAPPA, with an estimated sensitivity of <0.094ng/ml. The ELISA analytical biochemical technique of the MBS762955 kit is based on PAPPA antibody-PAPPA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PAPPA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PAPPA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for PAPPA elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- PAPPA antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- PAPPA antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PAPPA amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PAPPA can be calculated.
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 Typical Standard Curve/Testing Data of PAPPA elisa kit    PAPPA elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS762955. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PAPPA. It may not necessarily be applicable to this product.
 NCBI GI #    71025082
 NCBI GeneID    397219
 NCBI Accession #    AAZ17474.1 [Other Products]
 Molecular Weight    23,055 Da
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 NCBI Official Full Name    pregnancy associated plasma protein A, partial
 NCBI Official Symbol    PAPPA [Similar Products]
 NCBI Official Synonym Symbols   
PAPP-A
[Similar Products]
 NCBI Protein Information    pregnancy-associated plasma protein A, pappalysin 1; pregnancy associated plasma protein A; pregnancy-associated plasma protein Apappalysin 1; pregnancy-associated plasma protein-A
 UniProt Protein Name    Pregnancy associated plasma protein A
 UniProt Synonym Protein Names   
Pregnancy associated plasma protein A
 Protein Family    Pappalysin
 UniProt Entry Name    Q45XF7_PIG
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 UniProt Comments for PAPPA    Protein family/group databasesMEROPS protease database<br/><a href='/database/59'>More..</a>MEROPSiM43.004.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with PAPPA elisa kit
 Products by Pathway  Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway antibodies  Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway Diagram
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