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PYD elisa kit :: Rat Pyridinoline (PYD) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS266804
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $275 +1 FREE 8GB USB
96-Strip-Wells  /  $435 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Pyridinoline (PYD), ELISA Kit

★Popular Item★
 Also Known As   

Rat Pyridinoline (PYD) ELISA Kit

 Product Gene Name   

PYD elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    gene 614557
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 Species Reactivity    Rat
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 Specificity    No cross-reaction with other factors.
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 Samples    Serum, plasma or cell culture supernatant and organizations in the natural and recombinant PYD concentration.
 Assay Type    Sandwich
 Detection Range    400 nmol/L-6.25 nmol/L
 Sensitivity    1 nmol/L.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PYD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PYD purchase    MBS266804 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Pyridinoline (PYD) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 400 nmol/L-6.25 nmol/L in biological research samples containing PYD, with an estimated sensitivity of 1 nmol/L. The ELISA analytical biochemical technique of the MBS266804 kit is based on PYD antibody-PYD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PYD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PYD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma or cell culture supernatant and organizations in the natural and recombinant PYD concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PYD elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat PYD monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of PYD elisa kit    PYD elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS266804. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PYD. It may not necessarily be applicable to this product.
 NCBI GI #    23821568
 NCBI GeneID    55033
 NCBI Accession #    Q9NWM8.1 [Other Products]
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 UniProt Related Accession #    Q9NWM8 [Other Products]
 Molecular Weight    24,172 Da
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 NCBI Official Full Name    Peptidyl-prolyl cis-trans isomerase FKBP14
 NCBI Official Synonym Full Names    FK506 binding protein 14, 22 kDa
 NCBI Official Symbol    FKBP14 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    peptidyl-prolyl cis-trans isomerase FKBP14; FKBP-22; rotamase; 22 kDa FKBP; PPIase FKBP14; FK506-binding protein 14; 22 kDa FK506-binding protein
 UniProt Protein Name    Peptidyl-prolyl cis-trans isomerase FKBP14
 UniProt Synonym Protein Names   
22 kDa FK506-binding protein; 22 kDa FKBP; FKBP-22; FK506-binding protein 14; FKBP-14; Rotamase
 UniProt Gene Name    FKBP14 [Similar Products]
 UniProt Synonym Gene Names    FKBP22; UNQ322/PRO381; PPIase FKBP14; 22 kDa FKBP; FKBP-22; FKBP-14 [Similar Products]
 UniProt Entry Name    FKB14_HUMAN
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 NCBI Summary for PYD    The protein encoded by this gene is a member of the FK506-binding protein family of peptidyl-prolyl cis-trans isomerases. The encoded protein is found in the lumen of the endoplasmic reticulum, where it is thought to accelerate protein folding. Defects in this gene are a cause of a type of Ehlers-Danlos syndrome (EDS). Both a protein-coding variant and noncoding variants are transcribed from this gene. [provided by RefSeq, Mar 2012]
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 UniProt Comments for PYD    FKBP14: PPIases accelerate the folding of proteins during protein synthesis. Defects in FKBP14 are the cause of Ehlers-Danlos syndrome with progressive kyphoscoliosis, myopathy, and hearing loss (EDSKMH). A syndrome with features of Ehlers-Danlos syndrome types VIA and VIB on the one hand, and the collagen VI- related congenital myopathies Ullrich congenital muscular dystrophy and Bethlem myopathy on the other hand. Clinically, this disorder is characterized by the following features: severe generalized hypotonia at birth with marked muscle weakness that improve in infancy; early-onset progressive kyphoscoliosis; joint hypermobility without contractures; hyperelastic skin with follicular hyperkeratosis, easy bruising, and occasional abnormal scarring; myopathy as confirmed by muscle MRI, histology, and electron microscopy; hearing impairment, which is predominantly sensorineural; and a normal ratio of lysyl pyridinoline to hydroxylysyl pyridinoline (LP/HP) in urine.

Protein type: Isomerase; EC; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p14.3

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum lumen

Molecular Function: FK506 binding; peptidyl-prolyl cis-trans isomerase activity; calcium ion binding

Biological Process: cellular protein metabolic process; protein peptidyl-prolyl isomerization; unfolded protein response, activation of signaling protein activity; unfolded protein response

Disease: Ehlers-danlos Syndrome With Progressive Kyphoscoliosis, Myopathy, And Hearing Loss
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 Research Articles on PYD    1. A 1.9 A resolution crystal structure for human FKBP22 has been determined. The EF-hand motifs of two FKBP22 molecules form a dimeric complex with an elongated and predominantly hydrophobic cavity that can potentially be occupied by an aliphatic ligand.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with PYD elisa kitDiseases associated with PYD elisa kit
 Products by Pathway  Pathway Diagram
 IRE1alpha Activates Chaperones Pathway antibodies  IRE1alpha Activates Chaperones Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Unfolded Protein Response (UPR) Pathway antibodies  Unfolded Protein Response (UPR) Pathway Diagram
 XBP1(S) Activates Chaperone Genes Pathway antibodies  XBP1(S) Activates Chaperone Genes Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >2 publications with PYD and Nervous System Diseases
 Congenital Abnormalities Antibodies  >2 publications with PYD and Congenital Abnormalities
 Abnormalities, Multiple Antibodies  >2 publications with PYD and Abnormalities, Multiple
 Heart Diseases Antibodies  >1 publications with PYD and Heart Diseases
Organs/Tissues associated with PYD elisa kit
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >2 publications with PYD and Skin
 Connective Tissue Antibodies  >1 publications with PYD and Connective Tissue
 Vascular Antibodies  >1 publications with PYD and Vascular
 Lung Antibodies  >1 publications with PYD and Lung
 Eye Antibodies  >1 publications with PYD and Eye
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